A rapid near-patient detection system for SARS-CoV-2 using saliva

Abstract The highly infectious nature of SARS-CoV-2 necessitates the use of widespread testing to control the spread of the virus. Presently, the standard molecular testing method (reverse transcriptase-polymerase chain reaction, RT-PCR) is restricted to the laboratory, time-consuming, and costly. T...

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Autores principales:	Noah B. Toppings, Abu Naser Mohon, Yoonjung Lee, Hitendra Kumar, Daniel Lee, Ratik Kapoor, Gurmukh Singh, Lisa Oberding, Omar Abdullah, Keekyoung Kim, Byron M. Berenger, Dylan R. Pillai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e7751760c594819986c35de11671315
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spelling	oai:doaj.org-article:6e7751760c594819986c35de116713152021-12-02T16:32:12ZA rapid near-patient detection system for SARS-CoV-2 using saliva10.1038/s41598-021-92677-z2045-2322https://doaj.org/article/6e7751760c594819986c35de116713152021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92677-zhttps://doaj.org/toc/2045-2322Abstract The highly infectious nature of SARS-CoV-2 necessitates the use of widespread testing to control the spread of the virus. Presently, the standard molecular testing method (reverse transcriptase-polymerase chain reaction, RT-PCR) is restricted to the laboratory, time-consuming, and costly. This increases the turnaround time for getting test results. This study sought to develop a rapid, near-patient saliva-based test for COVID-19 (Saliva-Dry LAMP) with similar accuracy to that of standard RT-PCR tests. A lyophilized dual-target reverse transcription-loop-mediated isothermal amplification (RT-LAMP) test with fluorometric detection by the naked eye was developed. The assay relies on dry reagents that are room temperature stable. A device containing a centrifuge, heat block, and blue LED light system was manufactured to reduce the cost of performing the assay. This test has a limit of detection of 1 copy/µL and achieved a positive percent agreement of 100% [95% CI 88.43% to 100.0%] and a negative percent agreement of 96.7% [95% CI 82.78–99.92%] relative to a reference standard test. Saliva-Dry LAMP can be completed in 105 min. Precision, cross-reactivity, and interfering substances analysis met international regulatory standards. The combination of ease of sample collection, dry reagents, visual detection, low capital equipment cost, and excellent analytical sensitivity make Saliva-Dry LAMP particularly useful for resource-limited settings.Noah B. ToppingsAbu Naser MohonYoonjung LeeHitendra KumarDaniel LeeRatik KapoorGurmukh SinghLisa OberdingOmar AbdullahKeekyoung KimByron M. BerengerDylan R. PillaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Noah B. Toppings Abu Naser Mohon Yoonjung Lee Hitendra Kumar Daniel Lee Ratik Kapoor Gurmukh Singh Lisa Oberding Omar Abdullah Keekyoung Kim Byron M. Berenger Dylan R. Pillai A rapid near-patient detection system for SARS-CoV-2 using saliva
description	Abstract The highly infectious nature of SARS-CoV-2 necessitates the use of widespread testing to control the spread of the virus. Presently, the standard molecular testing method (reverse transcriptase-polymerase chain reaction, RT-PCR) is restricted to the laboratory, time-consuming, and costly. This increases the turnaround time for getting test results. This study sought to develop a rapid, near-patient saliva-based test for COVID-19 (Saliva-Dry LAMP) with similar accuracy to that of standard RT-PCR tests. A lyophilized dual-target reverse transcription-loop-mediated isothermal amplification (RT-LAMP) test with fluorometric detection by the naked eye was developed. The assay relies on dry reagents that are room temperature stable. A device containing a centrifuge, heat block, and blue LED light system was manufactured to reduce the cost of performing the assay. This test has a limit of detection of 1 copy/µL and achieved a positive percent agreement of 100% [95% CI 88.43% to 100.0%] and a negative percent agreement of 96.7% [95% CI 82.78–99.92%] relative to a reference standard test. Saliva-Dry LAMP can be completed in 105 min. Precision, cross-reactivity, and interfering substances analysis met international regulatory standards. The combination of ease of sample collection, dry reagents, visual detection, low capital equipment cost, and excellent analytical sensitivity make Saliva-Dry LAMP particularly useful for resource-limited settings.
format	article
author	Noah B. Toppings Abu Naser Mohon Yoonjung Lee Hitendra Kumar Daniel Lee Ratik Kapoor Gurmukh Singh Lisa Oberding Omar Abdullah Keekyoung Kim Byron M. Berenger Dylan R. Pillai
author_facet	Noah B. Toppings Abu Naser Mohon Yoonjung Lee Hitendra Kumar Daniel Lee Ratik Kapoor Gurmukh Singh Lisa Oberding Omar Abdullah Keekyoung Kim Byron M. Berenger Dylan R. Pillai
author_sort	Noah B. Toppings
title	A rapid near-patient detection system for SARS-CoV-2 using saliva
title_short	A rapid near-patient detection system for SARS-CoV-2 using saliva
title_full	A rapid near-patient detection system for SARS-CoV-2 using saliva
title_fullStr	A rapid near-patient detection system for SARS-CoV-2 using saliva
title_full_unstemmed	A rapid near-patient detection system for SARS-CoV-2 using saliva
title_sort	rapid near-patient detection system for sars-cov-2 using saliva
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6e7751760c594819986c35de11671315
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A rapid near-patient detection system for SARS-CoV-2 using saliva

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