Nanoporous solid-state membranes modified with multi-wall carbon nanotubes with anti-biofouling property

Ameneh Alizadeh,1 Amir Razmjou,1,2 Mehrorang Ghaedi,3 Ramin Jannesar4,51Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan 8174673441, Iran; 2UNESCO Centre for Membrane Science and Technology, School of Chemical Science and Engineering, Univers...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alizadeh A, Razmjou A, Ghaedi M, Jannesar R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Anodizing Alumina anodic membrane Antibacterial Anti-biofilm Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6e784253aee2406abdeb05e2fb0728b7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6e784253aee2406abdeb05e2fb0728b7
record_format	dspace
spelling	oai:doaj.org-article:6e784253aee2406abdeb05e2fb0728b72021-12-02T12:25:33ZNanoporous solid-state membranes modified with multi-wall carbon nanotubes with anti-biofouling property1178-2013https://doaj.org/article/6e784253aee2406abdeb05e2fb0728b72019-03-01T00:00:00Zhttps://www.dovepress.com/nanoporous-solid-state-membranes-modified-with-multi-wall-carbon-nanot-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Ameneh Alizadeh,1 Amir Razmjou,1,2 Mehrorang Ghaedi,3 Ramin Jannesar4,51Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan 8174673441, Iran; 2UNESCO Centre for Membrane Science and Technology, School of Chemical Science and Engineering, University of New South Wales, Sydney 2052, NSW, Australia; 3Department of Chemistry, Yasouj University, Yasouj 75918-74831, Iran; 4Department of Pathology, Yasuj University of Medical Sciences, Yasuj 7591741417, Iran; 5Department of Biotechnology and Microbial Nanotechnology, Dena Pathobiology Laboratory, Yasuj 7591774414, IranPurpose: Nanoporous membranes have been employing more than before in applications such as biomedical due to nanometer hexagonal pores array. Biofouling is one of the important problems in these applications that used nanoporous membranes and are in close contact with microorganisms. Surface modification of the membrane is one way to prevent biofilm formation; therefore, the membrane made in this work is modified with carbon nanotubes.Methods: In this work, nanoporous solid-state membrane (NSSM) was made by a two-step anodization method, and then modified with carbon nanotubes (NSSM-multi-wall carbon nanotubes [MWCNT]) by a simple chemical reaction. Techniques such as atomic force microscopy (AFM), energy dispersive X-ray (EDAX), field emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy (FTIR), contact angle (CA), surface free energy (SFE), protein adsorption, flow cytometry, and MTT assay were used for membrane characterization. Results: The BSA protein adsorption capacity reduced from 992.54 to 97.24 (µg mL-1 cm-2) after modification. The findings of flow cytometry and MTT assay confirmed that the number of dead bacteria was higher on the NSSM-MWCNT surface than that of control. Adsorption models of Freundlich and Langmuir and kinetics models were studied to understand the governing mechanism by which bacteria migrate to the membrane surface.Conclusion: The cell viability of absorbed bacteria on the NSSM-MWCNT was disrupted in direct physical contact with carbon nanotubes. Then, the dead bacteria were desorbed from the surface of the hydrophilic membrane. The results of this research showed that NSSM-MWCNT containing carbon nanotubes have significant antimicrobial and self-cleaning property that can be used in many biomedical devices without facing the eminent problem of biofouling. Keywords: anodizing, alumina anodic membrane, antibacterial, anti-biofilmAlizadeh ARazmjou AGhaedi MJannesar RDove Medical PressarticleAnodizingAlumina anodic membraneAntibacterialAnti-biofilmMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 1669-1685 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Anodizing Alumina anodic membrane Antibacterial Anti-biofilm Medicine (General) R5-920
spellingShingle	Anodizing Alumina anodic membrane Antibacterial Anti-biofilm Medicine (General) R5-920 Alizadeh A Razmjou A Ghaedi M Jannesar R Nanoporous solid-state membranes modified with multi-wall carbon nanotubes with anti-biofouling property
description	Ameneh Alizadeh,1 Amir Razmjou,1,2 Mehrorang Ghaedi,3 Ramin Jannesar4,51Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan 8174673441, Iran; 2UNESCO Centre for Membrane Science and Technology, School of Chemical Science and Engineering, University of New South Wales, Sydney 2052, NSW, Australia; 3Department of Chemistry, Yasouj University, Yasouj 75918-74831, Iran; 4Department of Pathology, Yasuj University of Medical Sciences, Yasuj 7591741417, Iran; 5Department of Biotechnology and Microbial Nanotechnology, Dena Pathobiology Laboratory, Yasuj 7591774414, IranPurpose: Nanoporous membranes have been employing more than before in applications such as biomedical due to nanometer hexagonal pores array. Biofouling is one of the important problems in these applications that used nanoporous membranes and are in close contact with microorganisms. Surface modification of the membrane is one way to prevent biofilm formation; therefore, the membrane made in this work is modified with carbon nanotubes.Methods: In this work, nanoporous solid-state membrane (NSSM) was made by a two-step anodization method, and then modified with carbon nanotubes (NSSM-multi-wall carbon nanotubes [MWCNT]) by a simple chemical reaction. Techniques such as atomic force microscopy (AFM), energy dispersive X-ray (EDAX), field emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy (FTIR), contact angle (CA), surface free energy (SFE), protein adsorption, flow cytometry, and MTT assay were used for membrane characterization. Results: The BSA protein adsorption capacity reduced from 992.54 to 97.24 (µg mL-1 cm-2) after modification. The findings of flow cytometry and MTT assay confirmed that the number of dead bacteria was higher on the NSSM-MWCNT surface than that of control. Adsorption models of Freundlich and Langmuir and kinetics models were studied to understand the governing mechanism by which bacteria migrate to the membrane surface.Conclusion: The cell viability of absorbed bacteria on the NSSM-MWCNT was disrupted in direct physical contact with carbon nanotubes. Then, the dead bacteria were desorbed from the surface of the hydrophilic membrane. The results of this research showed that NSSM-MWCNT containing carbon nanotubes have significant antimicrobial and self-cleaning property that can be used in many biomedical devices without facing the eminent problem of biofouling. Keywords: anodizing, alumina anodic membrane, antibacterial, anti-biofilm
format	article
author	Alizadeh A Razmjou A Ghaedi M Jannesar R
author_facet	Alizadeh A Razmjou A Ghaedi M Jannesar R
author_sort	Alizadeh A
title	Nanoporous solid-state membranes modified with multi-wall carbon nanotubes with anti-biofouling property
title_short	Nanoporous solid-state membranes modified with multi-wall carbon nanotubes with anti-biofouling property
title_full	Nanoporous solid-state membranes modified with multi-wall carbon nanotubes with anti-biofouling property
title_fullStr	Nanoporous solid-state membranes modified with multi-wall carbon nanotubes with anti-biofouling property
title_full_unstemmed	Nanoporous solid-state membranes modified with multi-wall carbon nanotubes with anti-biofouling property
title_sort	nanoporous solid-state membranes modified with multi-wall carbon nanotubes with anti-biofouling property
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/6e784253aee2406abdeb05e2fb0728b7
work_keys_str_mv	AT alizadeha nanoporoussolidstatemembranesmodifiedwithmultiwallcarbonnanotubeswithantibiofoulingproperty AT razmjoua nanoporoussolidstatemembranesmodifiedwithmultiwallcarbonnanotubeswithantibiofoulingproperty AT ghaedim nanoporoussolidstatemembranesmodifiedwithmultiwallcarbonnanotubeswithantibiofoulingproperty AT jannesarr nanoporoussolidstatemembranesmodifiedwithmultiwallcarbonnanotubeswithantibiofoulingproperty
_version_	1718394458961084416

Nanoporous solid-state membranes modified with multi-wall carbon nanotubes with anti-biofouling property

Ejemplares similares