Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum

Abstract Transmission of malaria parasites from humans to mosquito vectors requires that some asexual parasites differentiate into sexual forms termed gametocytes. The balance between proliferation in the same host and conversion into transmission forms can be altered by the conditions of the enviro...

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Autores principales:	Harvie P. Portugaliza, Oriol Llorà-Batlle, Anna Rosanas-Urgell, Alfred Cortés
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e79cd77fcd54caba7299c4a5b386e68
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spelling	oai:doaj.org-article:6e79cd77fcd54caba7299c4a5b386e682021-12-02T15:08:47ZReporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum10.1038/s41598-019-50768-y2045-2322https://doaj.org/article/6e79cd77fcd54caba7299c4a5b386e682019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-50768-yhttps://doaj.org/toc/2045-2322Abstract Transmission of malaria parasites from humans to mosquito vectors requires that some asexual parasites differentiate into sexual forms termed gametocytes. The balance between proliferation in the same host and conversion into transmission forms can be altered by the conditions of the environment. The ability to accurately measure the rate of sexual conversion under different conditions is essential for research addressing the mechanisms underlying sexual conversion, and to assess the impact of environmental factors. Here we describe new Plasmodium falciparum transgenic lines with genome-integrated constructs in which a fluorescent reporter is expressed under the control of the promoter of the gexp02 gene. Using these parasite lines, we developed a sexual conversion assay that shortens considerably the time needed for an accurate determination of sexual conversion rates, and dispenses the need to add chemicals to inhibit parasite replication. Furthermore, we demonstrate that gexp02 is expressed specifically in sexual parasites, with expression starting as early as the sexual ring stage, which makes it a candidate marker for circulating sexual rings in epidemiological studies.Harvie P. PortugalizaOriol Llorà-BatlleAnna Rosanas-UrgellAlfred CortésNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Harvie P. Portugaliza Oriol Llorà-Batlle Anna Rosanas-Urgell Alfred Cortés Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum
description	Abstract Transmission of malaria parasites from humans to mosquito vectors requires that some asexual parasites differentiate into sexual forms termed gametocytes. The balance between proliferation in the same host and conversion into transmission forms can be altered by the conditions of the environment. The ability to accurately measure the rate of sexual conversion under different conditions is essential for research addressing the mechanisms underlying sexual conversion, and to assess the impact of environmental factors. Here we describe new Plasmodium falciparum transgenic lines with genome-integrated constructs in which a fluorescent reporter is expressed under the control of the promoter of the gexp02 gene. Using these parasite lines, we developed a sexual conversion assay that shortens considerably the time needed for an accurate determination of sexual conversion rates, and dispenses the need to add chemicals to inhibit parasite replication. Furthermore, we demonstrate that gexp02 is expressed specifically in sexual parasites, with expression starting as early as the sexual ring stage, which makes it a candidate marker for circulating sexual rings in epidemiological studies.
format	article
author	Harvie P. Portugaliza Oriol Llorà-Batlle Anna Rosanas-Urgell Alfred Cortés
author_facet	Harvie P. Portugaliza Oriol Llorà-Batlle Anna Rosanas-Urgell Alfred Cortés
author_sort	Harvie P. Portugaliza
title	Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum
title_short	Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum
title_full	Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum
title_fullStr	Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum
title_full_unstemmed	Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum
title_sort	reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite plasmodium falciparum
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/6e79cd77fcd54caba7299c4a5b386e68
work_keys_str_mv	AT harviepportugaliza reporterlinesbasedonthegexp02promoterenableearlyquantificationofsexualconversionratesinthemalariaparasiteplasmodiumfalciparum AT oriolllorabatlle reporterlinesbasedonthegexp02promoterenableearlyquantificationofsexualconversionratesinthemalariaparasiteplasmodiumfalciparum AT annarosanasurgell reporterlinesbasedonthegexp02promoterenableearlyquantificationofsexualconversionratesinthemalariaparasiteplasmodiumfalciparum AT alfredcortes reporterlinesbasedonthegexp02promoterenableearlyquantificationofsexualconversionratesinthemalariaparasiteplasmodiumfalciparum
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Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum

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