Seed Micromorphology, In Vitro Germination, and Early-Stage Seedling Morphological Traits of Cattleya purpurata (Lindl. & Paxton) Van den Berg

In the context of a symbiotic plant-fungus interaction study concerning Cattleya purpurata, we focused on some aspects of seed morphology and biology, and the early stages of seedling development. Seed morphology was characterized using light and scanning electron microscopy. In v...

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Autores principales:	Miriam Bazzicalupo, Jacopo Calevo, Martino Adamo, Annalisa Giovannini, Andrea Copetta, Laura Cornara
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	crystals microscopy mycorrhizal fungi orchids suspensor Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/6e7aad0d7a6a498d90700fddf4e7f91d
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spelling	oai:doaj.org-article:6e7aad0d7a6a498d90700fddf4e7f91d2021-11-25T17:47:32ZSeed Micromorphology, <i>In Vitro</i> Germination, and Early-Stage Seedling Morphological Traits of <i>Cattleya purpurata</i> (Lindl. & Paxton) Van den Berg10.3390/horticulturae71104802311-7524https://doaj.org/article/6e7aad0d7a6a498d90700fddf4e7f91d2021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/480https://doaj.org/toc/2311-7524In the context of a symbiotic plant-fungus interaction study concerning <i>Cattleya purpurata</i>, we focused on some aspects of seed morphology and biology, and the early stages of seedling development. Seed morphology was characterized using light and scanning electron microscopy. In vitro seed germination capability was evaluated, comparing symbiotic and asymbiotic methods. The morphology of the seeds was overall comparable to that of other congeneric species, showing classical adaptations related to the aerodynamic properties and to the wettability of seeds, but calcium oxalate druses were identified inside the suspensor cells. Asymbiotic seed germination was successful in all tested media (17.1–46.5%) but was higher on 1/2 Murashige & Skoog. During symbiotic interaction with the fungal strain MUT4178 (<i>Tulasnella calospora</i>), germination rate was significantly lower than that obtained with the best three asymbiotic media, suggesting a low fungal compatibility. Seedling morphology was in line with other taxa from the same genus, showing typical characteristics of epiphytic species. Our observations, in particular, highlighted the presence of stomata with C-shaped guard cells in the leaves, rarely found in Cattleyas (where usually they are reniform), and confirm the presence of tilosomes in the roots. Idioblasts containing raphides were observed in both roots and leaves.Miriam BazzicalupoJacopo CalevoMartino AdamoAnnalisa GiovanniniAndrea CopettaLaura CornaraMDPI AGarticlecrystalsmicroscopymycorrhizal fungiorchidssuspensorPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 480, p 480 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	crystals microscopy mycorrhizal fungi orchids suspensor Plant culture SB1-1110
spellingShingle	crystals microscopy mycorrhizal fungi orchids suspensor Plant culture SB1-1110 Miriam Bazzicalupo Jacopo Calevo Martino Adamo Annalisa Giovannini Andrea Copetta Laura Cornara Seed Micromorphology, <i>In Vitro</i> Germination, and Early-Stage Seedling Morphological Traits of <i>Cattleya purpurata</i> (Lindl. & Paxton) Van den Berg
description	In the context of a symbiotic plant-fungus interaction study concerning <i>Cattleya purpurata</i>, we focused on some aspects of seed morphology and biology, and the early stages of seedling development. Seed morphology was characterized using light and scanning electron microscopy. In vitro seed germination capability was evaluated, comparing symbiotic and asymbiotic methods. The morphology of the seeds was overall comparable to that of other congeneric species, showing classical adaptations related to the aerodynamic properties and to the wettability of seeds, but calcium oxalate druses were identified inside the suspensor cells. Asymbiotic seed germination was successful in all tested media (17.1–46.5%) but was higher on 1/2 Murashige & Skoog. During symbiotic interaction with the fungal strain MUT4178 (<i>Tulasnella calospora</i>), germination rate was significantly lower than that obtained with the best three asymbiotic media, suggesting a low fungal compatibility. Seedling morphology was in line with other taxa from the same genus, showing typical characteristics of epiphytic species. Our observations, in particular, highlighted the presence of stomata with C-shaped guard cells in the leaves, rarely found in Cattleyas (where usually they are reniform), and confirm the presence of tilosomes in the roots. Idioblasts containing raphides were observed in both roots and leaves.
format	article
author	Miriam Bazzicalupo Jacopo Calevo Martino Adamo Annalisa Giovannini Andrea Copetta Laura Cornara
author_facet	Miriam Bazzicalupo Jacopo Calevo Martino Adamo Annalisa Giovannini Andrea Copetta Laura Cornara
author_sort	Miriam Bazzicalupo
title	Seed Micromorphology, <i>In Vitro</i> Germination, and Early-Stage Seedling Morphological Traits of <i>Cattleya purpurata</i> (Lindl. & Paxton) Van den Berg
title_short	Seed Micromorphology, <i>In Vitro</i> Germination, and Early-Stage Seedling Morphological Traits of <i>Cattleya purpurata</i> (Lindl. & Paxton) Van den Berg
title_full	Seed Micromorphology, <i>In Vitro</i> Germination, and Early-Stage Seedling Morphological Traits of <i>Cattleya purpurata</i> (Lindl. & Paxton) Van den Berg
title_fullStr	Seed Micromorphology, <i>In Vitro</i> Germination, and Early-Stage Seedling Morphological Traits of <i>Cattleya purpurata</i> (Lindl. & Paxton) Van den Berg
title_full_unstemmed	Seed Micromorphology, <i>In Vitro</i> Germination, and Early-Stage Seedling Morphological Traits of <i>Cattleya purpurata</i> (Lindl. & Paxton) Van den Berg
title_sort	seed micromorphology, <i>in vitro</i> germination, and early-stage seedling morphological traits of <i>cattleya purpurata</i> (lindl. & paxton) van den berg
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6e7aad0d7a6a498d90700fddf4e7f91d
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Seed Micromorphology, <i>In Vitro</i> Germination, and Early-Stage Seedling Morphological Traits of <i>Cattleya purpurata</i> (Lindl. & Paxton) Van den Berg

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