Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes

Abstract We have developed a novel method to deliver stem cells using 3D bioprinted cardiac patches, free of biomaterials. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), fibroblasts (FB) and endothelial cells (EC) were aggregated to create mixed cell spheroids. Cardiac patch...

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Autores principales:	Chin Siang Ong, Takuma Fukunishi, Huaitao Zhang, Chen Yu Huang, Andrew Nashed, Adriana Blazeski, Deborah DiSilvestre, Luca Vricella, John Conte, Leslie Tung, Gordon F. Tomaselli, Narutoshi Hibino
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e7b7ecb133a4b4abea17c9f5d020bd8
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spelling	oai:doaj.org-article:6e7b7ecb133a4b4abea17c9f5d020bd82021-12-02T15:06:14ZBiomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes10.1038/s41598-017-05018-42045-2322https://doaj.org/article/6e7b7ecb133a4b4abea17c9f5d020bd82017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05018-4https://doaj.org/toc/2045-2322Abstract We have developed a novel method to deliver stem cells using 3D bioprinted cardiac patches, free of biomaterials. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), fibroblasts (FB) and endothelial cells (EC) were aggregated to create mixed cell spheroids. Cardiac patches were created from spheroids (CM:FB:EC = 70:15:15, 70:0:30, 45:40:15) using a 3D bioprinter. Cardiac patches were analyzed with light and video microscopy, immunohistochemistry, immunofluorescence, cell viability assays and optical electrical mapping. Cardiac tissue patches of all cell ratios beat spontaneously after 3D bioprinting. Patches exhibited ventricular-like action potential waveforms and uniform electrical conduction throughout the patch. Conduction velocities were higher and action potential durations were significantly longer in patches containing a lower percentage of FBs. Immunohistochemistry revealed staining for CM, FB and EC markers, with rudimentary CD31+ blood vessel formation. Immunofluorescence revealed the presence of Cx43, the main cardiac gap junction protein, localized to cell-cell borders. In vivo implantation suggests vascularization of 3D bioprinted cardiac patches with engraftment into native rat myocardium. This constitutes a significant step towards a new generation of stem cell-based treatment for heart failure.Chin Siang OngTakuma FukunishiHuaitao ZhangChen Yu HuangAndrew NashedAdriana BlazeskiDeborah DiSilvestreLuca VricellaJohn ConteLeslie TungGordon F. TomaselliNarutoshi HibinoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chin Siang Ong Takuma Fukunishi Huaitao Zhang Chen Yu Huang Andrew Nashed Adriana Blazeski Deborah DiSilvestre Luca Vricella John Conte Leslie Tung Gordon F. Tomaselli Narutoshi Hibino Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes
description	Abstract We have developed a novel method to deliver stem cells using 3D bioprinted cardiac patches, free of biomaterials. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), fibroblasts (FB) and endothelial cells (EC) were aggregated to create mixed cell spheroids. Cardiac patches were created from spheroids (CM:FB:EC = 70:15:15, 70:0:30, 45:40:15) using a 3D bioprinter. Cardiac patches were analyzed with light and video microscopy, immunohistochemistry, immunofluorescence, cell viability assays and optical electrical mapping. Cardiac tissue patches of all cell ratios beat spontaneously after 3D bioprinting. Patches exhibited ventricular-like action potential waveforms and uniform electrical conduction throughout the patch. Conduction velocities were higher and action potential durations were significantly longer in patches containing a lower percentage of FBs. Immunohistochemistry revealed staining for CM, FB and EC markers, with rudimentary CD31+ blood vessel formation. Immunofluorescence revealed the presence of Cx43, the main cardiac gap junction protein, localized to cell-cell borders. In vivo implantation suggests vascularization of 3D bioprinted cardiac patches with engraftment into native rat myocardium. This constitutes a significant step towards a new generation of stem cell-based treatment for heart failure.
format	article
author	Chin Siang Ong Takuma Fukunishi Huaitao Zhang Chen Yu Huang Andrew Nashed Adriana Blazeski Deborah DiSilvestre Luca Vricella John Conte Leslie Tung Gordon F. Tomaselli Narutoshi Hibino
author_facet	Chin Siang Ong Takuma Fukunishi Huaitao Zhang Chen Yu Huang Andrew Nashed Adriana Blazeski Deborah DiSilvestre Luca Vricella John Conte Leslie Tung Gordon F. Tomaselli Narutoshi Hibino
author_sort	Chin Siang Ong
title	Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes
title_short	Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes
title_full	Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes
title_fullStr	Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes
title_full_unstemmed	Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes
title_sort	biomaterial-free three-dimensional bioprinting of cardiac tissue using human induced pluripotent stem cell derived cardiomyocytes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6e7b7ecb133a4b4abea17c9f5d020bd8
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Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes

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