Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes

Abstract We have developed a novel method to deliver stem cells using 3D bioprinted cardiac patches, free of biomaterials. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), fibroblasts (FB) and endothelial cells (EC) were aggregated to create mixed cell spheroids. Cardiac patch...

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Autores principales: Chin Siang Ong, Takuma Fukunishi, Huaitao Zhang, Chen Yu Huang, Andrew Nashed, Adriana Blazeski, Deborah DiSilvestre, Luca Vricella, John Conte, Leslie Tung, Gordon F. Tomaselli, Narutoshi Hibino
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/6e7b7ecb133a4b4abea17c9f5d020bd8
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spelling oai:doaj.org-article:6e7b7ecb133a4b4abea17c9f5d020bd82021-12-02T15:06:14ZBiomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes10.1038/s41598-017-05018-42045-2322https://doaj.org/article/6e7b7ecb133a4b4abea17c9f5d020bd82017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05018-4https://doaj.org/toc/2045-2322Abstract We have developed a novel method to deliver stem cells using 3D bioprinted cardiac patches, free of biomaterials. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), fibroblasts (FB) and endothelial cells (EC) were aggregated to create mixed cell spheroids. Cardiac patches were created from spheroids (CM:FB:EC = 70:15:15, 70:0:30, 45:40:15) using a 3D bioprinter. Cardiac patches were analyzed with light and video microscopy, immunohistochemistry, immunofluorescence, cell viability assays and optical electrical mapping. Cardiac tissue patches of all cell ratios beat spontaneously after 3D bioprinting. Patches exhibited ventricular-like action potential waveforms and uniform electrical conduction throughout the patch. Conduction velocities were higher and action potential durations were significantly longer in patches containing a lower percentage of FBs. Immunohistochemistry revealed staining for CM, FB and EC markers, with rudimentary CD31+ blood vessel formation. Immunofluorescence revealed the presence of Cx43, the main cardiac gap junction protein, localized to cell-cell borders. In vivo implantation suggests vascularization of 3D bioprinted cardiac patches with engraftment into native rat myocardium. This constitutes a significant step towards a new generation of stem cell-based treatment for heart failure.Chin Siang OngTakuma FukunishiHuaitao ZhangChen Yu HuangAndrew NashedAdriana BlazeskiDeborah DiSilvestreLuca VricellaJohn ConteLeslie TungGordon F. TomaselliNarutoshi HibinoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Chin Siang Ong
Takuma Fukunishi
Huaitao Zhang
Chen Yu Huang
Andrew Nashed
Adriana Blazeski
Deborah DiSilvestre
Luca Vricella
John Conte
Leslie Tung
Gordon F. Tomaselli
Narutoshi Hibino
Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes
description Abstract We have developed a novel method to deliver stem cells using 3D bioprinted cardiac patches, free of biomaterials. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs), fibroblasts (FB) and endothelial cells (EC) were aggregated to create mixed cell spheroids. Cardiac patches were created from spheroids (CM:FB:EC = 70:15:15, 70:0:30, 45:40:15) using a 3D bioprinter. Cardiac patches were analyzed with light and video microscopy, immunohistochemistry, immunofluorescence, cell viability assays and optical electrical mapping. Cardiac tissue patches of all cell ratios beat spontaneously after 3D bioprinting. Patches exhibited ventricular-like action potential waveforms and uniform electrical conduction throughout the patch. Conduction velocities were higher and action potential durations were significantly longer in patches containing a lower percentage of FBs. Immunohistochemistry revealed staining for CM, FB and EC markers, with rudimentary CD31+ blood vessel formation. Immunofluorescence revealed the presence of Cx43, the main cardiac gap junction protein, localized to cell-cell borders. In vivo implantation suggests vascularization of 3D bioprinted cardiac patches with engraftment into native rat myocardium. This constitutes a significant step towards a new generation of stem cell-based treatment for heart failure.
format article
author Chin Siang Ong
Takuma Fukunishi
Huaitao Zhang
Chen Yu Huang
Andrew Nashed
Adriana Blazeski
Deborah DiSilvestre
Luca Vricella
John Conte
Leslie Tung
Gordon F. Tomaselli
Narutoshi Hibino
author_facet Chin Siang Ong
Takuma Fukunishi
Huaitao Zhang
Chen Yu Huang
Andrew Nashed
Adriana Blazeski
Deborah DiSilvestre
Luca Vricella
John Conte
Leslie Tung
Gordon F. Tomaselli
Narutoshi Hibino
author_sort Chin Siang Ong
title Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes
title_short Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes
title_full Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes
title_fullStr Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes
title_full_unstemmed Biomaterial-Free Three-Dimensional Bioprinting of Cardiac Tissue using Human Induced Pluripotent Stem Cell Derived Cardiomyocytes
title_sort biomaterial-free three-dimensional bioprinting of cardiac tissue using human induced pluripotent stem cell derived cardiomyocytes
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/6e7b7ecb133a4b4abea17c9f5d020bd8
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