FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis

Abstract Flagellin is amongst the most abundant proteins in flagellated bacterial species and constitutes the major building block of the flagellar filament. The proteins FliW and FliS serve in the post-transcriptional control of flagellin and guide the protein to the flagellar type III secretion sy...

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Autores principales:	Florian Altegoer, Sampriti Mukherjee, Wieland Steinchen, Patricia Bedrunka, Uwe Linne, Daniel B. Kearns, Gert Bange
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e8005bd2c804e068d9438aadc41f49d
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spelling	oai:doaj.org-article:6e8005bd2c804e068d9438aadc41f49d2021-12-02T15:07:45ZFliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis10.1038/s41598-018-29884-82045-2322https://doaj.org/article/6e8005bd2c804e068d9438aadc41f49d2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29884-8https://doaj.org/toc/2045-2322Abstract Flagellin is amongst the most abundant proteins in flagellated bacterial species and constitutes the major building block of the flagellar filament. The proteins FliW and FliS serve in the post-transcriptional control of flagellin and guide the protein to the flagellar type III secretion system (fT3SS), respectively. Here, we present the high-resolution structure of FliS/flagellin heterodimer and show that FliS and FliW bind to opposing interfaces located at the N- and C-termini of flagellin. The FliS/flagellin/FliW heterotrimer is able to interact with FlhA-C suggesting that FliW and FliS are released during flagellin export. After release, FliW and FliS are recycled to execute a new round of post-transcriptional regulation and targeting. Taken together, our study provides a mechanism explaining how FliW and FliS synchronize the production of flagellin with the capacity of the fT3SS to secrete flagellin.Florian AltegoerSampriti MukherjeeWieland SteinchenPatricia BedrunkaUwe LinneDaniel B. KearnsGert BangeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Florian Altegoer Sampriti Mukherjee Wieland Steinchen Patricia Bedrunka Uwe Linne Daniel B. Kearns Gert Bange FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis
description	Abstract Flagellin is amongst the most abundant proteins in flagellated bacterial species and constitutes the major building block of the flagellar filament. The proteins FliW and FliS serve in the post-transcriptional control of flagellin and guide the protein to the flagellar type III secretion system (fT3SS), respectively. Here, we present the high-resolution structure of FliS/flagellin heterodimer and show that FliS and FliW bind to opposing interfaces located at the N- and C-termini of flagellin. The FliS/flagellin/FliW heterotrimer is able to interact with FlhA-C suggesting that FliW and FliS are released during flagellin export. After release, FliW and FliS are recycled to execute a new round of post-transcriptional regulation and targeting. Taken together, our study provides a mechanism explaining how FliW and FliS synchronize the production of flagellin with the capacity of the fT3SS to secrete flagellin.
format	article
author	Florian Altegoer Sampriti Mukherjee Wieland Steinchen Patricia Bedrunka Uwe Linne Daniel B. Kearns Gert Bange
author_facet	Florian Altegoer Sampriti Mukherjee Wieland Steinchen Patricia Bedrunka Uwe Linne Daniel B. Kearns Gert Bange
author_sort	Florian Altegoer
title	FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis
title_short	FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis
title_full	FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis
title_fullStr	FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis
title_full_unstemmed	FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis
title_sort	flis/flagellin/fliw heterotrimer couples type iii secretion and flagellin homeostasis
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6e8005bd2c804e068d9438aadc41f49d
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FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis

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