Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria

Abstract Mycobacteria are classified into two groups, fast- and slow-growing. Often, fast-growing mycobacteria are assumed to have a higher metabolic activity than their slower counterparts, but in mature biofilms this assumption might not be correct. Indeed, when measuring the metabolic activity of...

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Autores principales:	Anna Solokhina, David Brückner, Gernot Bonkat, Olivier Braissant
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e8d00900df947d5bb616b3b2707838a
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spelling	oai:doaj.org-article:6e8d00900df947d5bb616b3b2707838a2021-12-02T15:06:26ZMetabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria10.1038/s41598-017-10019-42045-2322https://doaj.org/article/6e8d00900df947d5bb616b3b2707838a2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-10019-4https://doaj.org/toc/2045-2322Abstract Mycobacteria are classified into two groups, fast- and slow-growing. Often, fast-growing mycobacteria are assumed to have a higher metabolic activity than their slower counterparts, but in mature biofilms this assumption might not be correct. Indeed, when measuring the metabolic activity of mycobacterial biofilms with two independent non-invasive techniques (isothermal microcalorimetry and tunable diode laser absorption spectrometry), mature biofilms of slow- and fast-growing species appeared more alike than expected. Metabolic heat production rate was 2298 ± 181 µW for M. smegmatis and 792 ± 81 µW for M. phlei, while M. tuberculosis and M. bovis metabolic heat production rates were between these values. These small differences were further confirmed by similar oxygen consumption rates (3.3 ± 0.2 nMole/s and 1.7 ± 0.3 nMole/s for M. smegmatis and M. tuberculosis, respectively). These data suggest that the metabolic potential of slow-growing mycobacterial biofilms has been underestimated, particularly for pathogenic species.Anna SolokhinaDavid BrücknerGernot BonkatOlivier BraissantNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Solokhina David Brückner Gernot Bonkat Olivier Braissant Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria
description	Abstract Mycobacteria are classified into two groups, fast- and slow-growing. Often, fast-growing mycobacteria are assumed to have a higher metabolic activity than their slower counterparts, but in mature biofilms this assumption might not be correct. Indeed, when measuring the metabolic activity of mycobacterial biofilms with two independent non-invasive techniques (isothermal microcalorimetry and tunable diode laser absorption spectrometry), mature biofilms of slow- and fast-growing species appeared more alike than expected. Metabolic heat production rate was 2298 ± 181 µW for M. smegmatis and 792 ± 81 µW for M. phlei, while M. tuberculosis and M. bovis metabolic heat production rates were between these values. These small differences were further confirmed by similar oxygen consumption rates (3.3 ± 0.2 nMole/s and 1.7 ± 0.3 nMole/s for M. smegmatis and M. tuberculosis, respectively). These data suggest that the metabolic potential of slow-growing mycobacterial biofilms has been underestimated, particularly for pathogenic species.
format	article
author	Anna Solokhina David Brückner Gernot Bonkat Olivier Braissant
author_facet	Anna Solokhina David Brückner Gernot Bonkat Olivier Braissant
author_sort	Anna Solokhina
title	Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria
title_short	Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria
title_full	Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria
title_fullStr	Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria
title_full_unstemmed	Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria
title_sort	metabolic activity of mature biofilms of mycobacterium tuberculosis and other non-tuberculous mycobacteria
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6e8d00900df947d5bb616b3b2707838a
work_keys_str_mv	AT annasolokhina metabolicactivityofmaturebiofilmsofmycobacteriumtuberculosisandothernontuberculousmycobacteria AT davidbruckner metabolicactivityofmaturebiofilmsofmycobacteriumtuberculosisandothernontuberculousmycobacteria AT gernotbonkat metabolicactivityofmaturebiofilmsofmycobacteriumtuberculosisandothernontuberculousmycobacteria AT olivierbraissant metabolicactivityofmaturebiofilmsofmycobacteriumtuberculosisandothernontuberculousmycobacteria
_version_	1718388454423789568

Metabolic activity of mature biofilms of Mycobacterium tuberculosis and other non-tuberculous mycobacteria

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