Facile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity

Abstract A facile method for generation of tumor spheroids in large quantity with controllable size and high uniformity is presented. HCT-116 cells are used as a model cell line. Individual tumor cells are sparsely seeded onto petri-dishes. After a few days of growth, separated cellular islets are f...

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Autores principales:	Wentao Shi, Jean Kwon, Yongyang Huang, Jifu Tan, Christopher G. Uhl, Ran He, Chao Zhou, Yaling Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6e9f196f95c54b0eaee8ea5759b01625
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spelling	oai:doaj.org-article:6e9f196f95c54b0eaee8ea5759b016252021-12-02T12:31:56ZFacile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity10.1038/s41598-018-25203-32045-2322https://doaj.org/article/6e9f196f95c54b0eaee8ea5759b016252018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25203-3https://doaj.org/toc/2045-2322Abstract A facile method for generation of tumor spheroids in large quantity with controllable size and high uniformity is presented. HCT-116 cells are used as a model cell line. Individual tumor cells are sparsely seeded onto petri-dishes. After a few days of growth, separated cellular islets are formed and then detached by dispase while maintaining their sheet shape. These detached cell sheets are transferred to dispase-doped media under orbital shaking conditions. Assisted by the shear flow under shaking and inhibition of cell-to-extracellular matrix junctions by dispase, the cell sheets curl up and eventually tumor spheroids are formed. The average size of the spheroids can be controlled by tuning the cell sheet culturing period and spheroid shaking period. The uniformity can be controlled by a set of sieves which were home-made using stainless steel meshes. Since this method is based on simple petri-dish cell culturing and shaking, it is rather facile for forming tumor spheroids with no theoretical quantity limit. This method has been used to form HeLa, A431 and U87 MG tumor spheroids and application of the formed tumor spheroids in drug screening is also demonstrated. The viability, 3D structure, and necrosis of the spheroids are characterized.Wentao ShiJean KwonYongyang HuangJifu TanChristopher G. UhlRan HeChao ZhouYaling LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wentao Shi Jean Kwon Yongyang Huang Jifu Tan Christopher G. Uhl Ran He Chao Zhou Yaling Liu Facile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity
description	Abstract A facile method for generation of tumor spheroids in large quantity with controllable size and high uniformity is presented. HCT-116 cells are used as a model cell line. Individual tumor cells are sparsely seeded onto petri-dishes. After a few days of growth, separated cellular islets are formed and then detached by dispase while maintaining their sheet shape. These detached cell sheets are transferred to dispase-doped media under orbital shaking conditions. Assisted by the shear flow under shaking and inhibition of cell-to-extracellular matrix junctions by dispase, the cell sheets curl up and eventually tumor spheroids are formed. The average size of the spheroids can be controlled by tuning the cell sheet culturing period and spheroid shaking period. The uniformity can be controlled by a set of sieves which were home-made using stainless steel meshes. Since this method is based on simple petri-dish cell culturing and shaking, it is rather facile for forming tumor spheroids with no theoretical quantity limit. This method has been used to form HeLa, A431 and U87 MG tumor spheroids and application of the formed tumor spheroids in drug screening is also demonstrated. The viability, 3D structure, and necrosis of the spheroids are characterized.
format	article
author	Wentao Shi Jean Kwon Yongyang Huang Jifu Tan Christopher G. Uhl Ran He Chao Zhou Yaling Liu
author_facet	Wentao Shi Jean Kwon Yongyang Huang Jifu Tan Christopher G. Uhl Ran He Chao Zhou Yaling Liu
author_sort	Wentao Shi
title	Facile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity
title_short	Facile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity
title_full	Facile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity
title_fullStr	Facile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity
title_full_unstemmed	Facile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity
title_sort	facile tumor spheroids formation in large quantity with controllable size and high uniformity
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6e9f196f95c54b0eaee8ea5759b01625
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Facile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity

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