Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.

Very small embryonic-like (VSEL) cells have been described as putatively pluripotent stem cells present in murine bone marrow and human umbilical cord blood (hUCB) and as such are of high potential interest for regenerative medicine. However, there remain some questions concerning the precise identi...

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Autores principales:	Ralitza Danova-Alt, Andreas Heider, Dietmar Egger, Michael Cross, Rüdiger Alt
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ea433827a37413da5b47c0efcfda5f0
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spelling	oai:doaj.org-article:6ea433827a37413da5b47c0efcfda5f02021-11-18T07:23:20ZVery small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.1932-620310.1371/journal.pone.0034899https://doaj.org/article/6ea433827a37413da5b47c0efcfda5f02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22509366/?tool=EBIhttps://doaj.org/toc/1932-6203Very small embryonic-like (VSEL) cells have been described as putatively pluripotent stem cells present in murine bone marrow and human umbilical cord blood (hUCB) and as such are of high potential interest for regenerative medicine. However, there remain some questions concerning the precise identity and properties of VSEL cells, particularly those derived from hUCB. For this reason, we have carried out an extensive characterisation of purified populations of VSEL cells from a large number of UCB samples. Consistent with a previous report, we find that VSEL cells are CXCR4(+), have a high density, are indeed significantly smaller than HSC and have an extremely high nuclear/cytoplasmic ratio. Their nucleoplasm is unstructured and stains strongly with Hoechst 33342. A comprehensive FACS screen for surface markers characteristic of embryonic, mesenchymal, neuronal or hematopoietic stem cells revealed negligible expression on VSEL cells. These cells failed to expand in vitro under a wide range of culture conditions known to support embryonic or adult stem cell types and a microarray analysis revealed the transcriptional profile of VSEL cells to be clearly distinct both from well-defined populations of pluripotent and adult stem cells and from the mature hematopoietic lineages. Finally, we detected an aneuploid karyotype in the majority of purified VSEL cells by fluorescence in situ hybridisation. These data support neither an embryonic nor an adult stem cell like phenotype, suggesting rather that hUCB VSEL cells are an aberrant and inactive population that is not comparable to murine VSEL cells.Ralitza Danova-AltAndreas HeiderDietmar EggerMichael CrossRüdiger AltPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e34899 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ralitza Danova-Alt Andreas Heider Dietmar Egger Michael Cross Rüdiger Alt Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.
description	Very small embryonic-like (VSEL) cells have been described as putatively pluripotent stem cells present in murine bone marrow and human umbilical cord blood (hUCB) and as such are of high potential interest for regenerative medicine. However, there remain some questions concerning the precise identity and properties of VSEL cells, particularly those derived from hUCB. For this reason, we have carried out an extensive characterisation of purified populations of VSEL cells from a large number of UCB samples. Consistent with a previous report, we find that VSEL cells are CXCR4(+), have a high density, are indeed significantly smaller than HSC and have an extremely high nuclear/cytoplasmic ratio. Their nucleoplasm is unstructured and stains strongly with Hoechst 33342. A comprehensive FACS screen for surface markers characteristic of embryonic, mesenchymal, neuronal or hematopoietic stem cells revealed negligible expression on VSEL cells. These cells failed to expand in vitro under a wide range of culture conditions known to support embryonic or adult stem cell types and a microarray analysis revealed the transcriptional profile of VSEL cells to be clearly distinct both from well-defined populations of pluripotent and adult stem cells and from the mature hematopoietic lineages. Finally, we detected an aneuploid karyotype in the majority of purified VSEL cells by fluorescence in situ hybridisation. These data support neither an embryonic nor an adult stem cell like phenotype, suggesting rather that hUCB VSEL cells are an aberrant and inactive population that is not comparable to murine VSEL cells.
format	article
author	Ralitza Danova-Alt Andreas Heider Dietmar Egger Michael Cross Rüdiger Alt
author_facet	Ralitza Danova-Alt Andreas Heider Dietmar Egger Michael Cross Rüdiger Alt
author_sort	Ralitza Danova-Alt
title	Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.
title_short	Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.
title_full	Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.
title_fullStr	Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.
title_full_unstemmed	Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.
title_sort	very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6ea433827a37413da5b47c0efcfda5f0
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Very small embryonic-like stem cells purified from umbilical cord blood lack stem cell characteristics.

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