Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects

Adithan Aravinthan,1,* Muthusamy Govarthanan,2,3,* Kandasamy Selvam,4 Loganathan Praburaman,2,3 Thangasamy Selvankumar,3 Rangachari Balamurugan,1 Seralathan Kamala-Kannan,2 Jong-Hoon Kim1 1College of Veterinary Medicine, Biosafety Research Institute, Chonbuk National University, Jeonju, South Kore...

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Autores principales:	Aravinthan A, Govarthanan M, Selvam K, Praburaman L, Selvankumar T, Balamurugan R, Kamala-Kannan S, Kim JH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6ea5a045cc7d4e198b0662d0f80b745d
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spelling	oai:doaj.org-article:6ea5a045cc7d4e198b0662d0f80b745d2021-12-02T00:38:09ZSunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects1178-2013https://doaj.org/article/6ea5a045cc7d4e198b0662d0f80b745d2015-03-01T00:00:00Zhttp://www.dovepress.com/sunroot-mediated-synthesis-and-characterization-of-silver-nanoparticle-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Adithan Aravinthan,1,* Muthusamy Govarthanan,2,3,* Kandasamy Selvam,4 Loganathan Praburaman,2,3 Thangasamy Selvankumar,3 Rangachari Balamurugan,1 Seralathan Kamala-Kannan,2 Jong-Hoon Kim1 1College of Veterinary Medicine, Biosafety Research Institute, Chonbuk National University, Jeonju, South Korea; 2Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea; 3PG and Research Department of Biotechnology, Mahendra Arts and Science College, Kalippatti, Namakkal, Tamil Nadu, India; 4Centre for Biotechnology, Muthayammal College of Arts and Science, Rasipuram, Namakkal, Tamil Nadu, India *These authors contributed equally to this work Abstract: A rapid, green phytosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of Helianthus tuberosus (sunroot tuber) was reported in this study. The morphology of the AgNPs was determined by transmission electron microscopy (TEM). Scanning electron microscopy–energy-dispersive spectroscopy (SEM–EDS) and X-ray powder diffraction (XRD) analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy (FTIR) analysis revealed that biomolecules in the tuber extract were involved in the reduction and capping of AgNPs. The energy-dispersive spectroscopy (EDS) analysis of the AgNPs, using an energy range of 2–4 keV, confirmed the presence of elemental silver without any contamination. Further, the synthesized AgNPs were evaluated against phytopathogens such as Ralstonia solanacearum and Xanthomonas axonopodis. The AgNPs (1–4 mM) extensively reduced the growth rate of the phytopathogens. In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes. The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 µg/mL. Keywords: cytotoxicity, Helianthus tuberosus, nanobiotechnology, phytosynthesis, splenocytesAravinthan AGovarthanan MSelvam KPraburaman LSelvankumar TBalamurugan RKamala-Kannan SKim JHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1977-1983 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Aravinthan A Govarthanan M Selvam K Praburaman L Selvankumar T Balamurugan R Kamala-Kannan S Kim JH Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects
description	Adithan Aravinthan,1,* Muthusamy Govarthanan,2,3,* Kandasamy Selvam,4 Loganathan Praburaman,2,3 Thangasamy Selvankumar,3 Rangachari Balamurugan,1 Seralathan Kamala-Kannan,2 Jong-Hoon Kim1 1College of Veterinary Medicine, Biosafety Research Institute, Chonbuk National University, Jeonju, South Korea; 2Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, South Korea; 3PG and Research Department of Biotechnology, Mahendra Arts and Science College, Kalippatti, Namakkal, Tamil Nadu, India; 4Centre for Biotechnology, Muthayammal College of Arts and Science, Rasipuram, Namakkal, Tamil Nadu, India *These authors contributed equally to this work Abstract: A rapid, green phytosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of Helianthus tuberosus (sunroot tuber) was reported in this study. The morphology of the AgNPs was determined by transmission electron microscopy (TEM). Scanning electron microscopy–energy-dispersive spectroscopy (SEM–EDS) and X-ray powder diffraction (XRD) analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy (FTIR) analysis revealed that biomolecules in the tuber extract were involved in the reduction and capping of AgNPs. The energy-dispersive spectroscopy (EDS) analysis of the AgNPs, using an energy range of 2–4 keV, confirmed the presence of elemental silver without any contamination. Further, the synthesized AgNPs were evaluated against phytopathogens such as Ralstonia solanacearum and Xanthomonas axonopodis. The AgNPs (1–4 mM) extensively reduced the growth rate of the phytopathogens. In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes. The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 µg/mL. Keywords: cytotoxicity, Helianthus tuberosus, nanobiotechnology, phytosynthesis, splenocytes
format	article
author	Aravinthan A Govarthanan M Selvam K Praburaman L Selvankumar T Balamurugan R Kamala-Kannan S Kim JH
author_facet	Aravinthan A Govarthanan M Selvam K Praburaman L Selvankumar T Balamurugan R Kamala-Kannan S Kim JH
author_sort	Aravinthan A
title	Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects
title_short	Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects
title_full	Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects
title_fullStr	Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects
title_full_unstemmed	Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects
title_sort	sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/6ea5a045cc7d4e198b0662d0f80b745d
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Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects

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