Structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase

Core-fucosylation of the N-glycan core is an essential biological modification and the α1,6- fucosyltransferase FUT8 is the only enzyme in humans that catalyses this modification through the addition of an α-1,6-linked fucose to N-glycans. Here the authors provide insights into FUT8 substrate recogn...

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Autores principales: Ana García-García, Laura Ceballos-Laita, Sonia Serna, Raik Artschwager, Niels C. Reichardt, Francisco Corzana, Ramon Hurtado-Guerrero
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Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/6eaef7c9348f45cc8e916ae6a380340a
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spelling oai:doaj.org-article:6eaef7c9348f45cc8e916ae6a380340a2021-12-02T16:49:14ZStructural basis for substrate specificity and catalysis of α1,6-fucosyltransferase10.1038/s41467-020-14794-z2041-1723https://doaj.org/article/6eaef7c9348f45cc8e916ae6a380340a2020-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-14794-zhttps://doaj.org/toc/2041-1723Core-fucosylation of the N-glycan core is an essential biological modification and the α1,6- fucosyltransferase FUT8 is the only enzyme in humans that catalyses this modification through the addition of an α-1,6-linked fucose to N-glycans. Here the authors provide insights into FUT8 substrate recognition by determining the 1.95 Å crystal structure of human FUT8 complexed with GDP and a biantennary complex N-glycan.Ana García-GarcíaLaura Ceballos-LaitaSonia SernaRaik ArtschwagerNiels C. ReichardtFrancisco CorzanaRamon Hurtado-GuerreroNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Ana García-García
Laura Ceballos-Laita
Sonia Serna
Raik Artschwager
Niels C. Reichardt
Francisco Corzana
Ramon Hurtado-Guerrero
Structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase
description Core-fucosylation of the N-glycan core is an essential biological modification and the α1,6- fucosyltransferase FUT8 is the only enzyme in humans that catalyses this modification through the addition of an α-1,6-linked fucose to N-glycans. Here the authors provide insights into FUT8 substrate recognition by determining the 1.95 Å crystal structure of human FUT8 complexed with GDP and a biantennary complex N-glycan.
format article
author Ana García-García
Laura Ceballos-Laita
Sonia Serna
Raik Artschwager
Niels C. Reichardt
Francisco Corzana
Ramon Hurtado-Guerrero
author_facet Ana García-García
Laura Ceballos-Laita
Sonia Serna
Raik Artschwager
Niels C. Reichardt
Francisco Corzana
Ramon Hurtado-Guerrero
author_sort Ana García-García
title Structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase
title_short Structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase
title_full Structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase
title_fullStr Structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase
title_full_unstemmed Structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase
title_sort structural basis for substrate specificity and catalysis of α1,6-fucosyltransferase
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/6eaef7c9348f45cc8e916ae6a380340a
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