Regulatory Effect of Anwulignan on the Immune Function Through Its Antioxidation and Anti-Apoptosis in D-Galactose-Induced Aging Mice

Xin Li,1 Jiaqi Gao,1 Zepeng Yu,1 Weihai Jiang,2 Wei Sun,2 Chunyan Yu,1 Jinghui Sun,1 Chunmei Wang,1 Jianguang Chen,1 Shu Jing,2 He Li1 1Department of Pharmacology, College of Pharmacy, Beihua University, Jilin, Jilin 132013, People’s Republic of China; 2Affiliated Hospital of Beihua Univer...

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Autores principales:	Li X, Gao J, Yu Z, Jiang W, Sun W, Yu C, Sun J, Wang C, Chen J, Jing S, Li H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	anwulignan immunosenescence antioxidation anti-apoptosis Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/6ec8c79bbaf34f1aa688838cc251c250
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spelling	oai:doaj.org-article:6ec8c79bbaf34f1aa688838cc251c2502021-12-02T05:06:41ZRegulatory Effect of Anwulignan on the Immune Function Through Its Antioxidation and Anti-Apoptosis in D-Galactose-Induced Aging Mice1178-1998https://doaj.org/article/6ec8c79bbaf34f1aa688838cc251c2502020-01-01T00:00:00Zhttps://www.dovepress.com/regulatory-effect-of-anwulignan-on-the-immune-function-through-its-ant-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Xin Li,1 Jiaqi Gao,1 Zepeng Yu,1 Weihai Jiang,2 Wei Sun,2 Chunyan Yu,1 Jinghui Sun,1 Chunmei Wang,1 Jianguang Chen,1 Shu Jing,2 He Li1 1Department of Pharmacology, College of Pharmacy, Beihua University, Jilin, Jilin 132013, People’s Republic of China; 2Affiliated Hospital of Beihua University, Jilin, Jilin 132011, People’s Republic of ChinaCorrespondence: Shu Jing; He Li Email yitongjingshu@163.com; yitonglh@126.comBackground: Aging is a spontaneous and inevitable phenomenon of biology, which can lead to the gradual deterioration of tissues and organs. One of the age-related deterioration processes is immunosenescence, which leads to changes in the function of immune systems, including immune cells and associated cytokines. A proper modulation of immune responses can improve the age-related immunosenescence process and then reach healthy aging. Schisandra sphenanthera, a traditional Chinese medicine, has been used as both a medicine and a nutritional supplement for thousands of years. Anwulignan, a monomer compound of Schisandra sphenanthera lignans, has been reported to possess an immunomodulatory effect. Therefore, this study was designed to further explore whether Anwulignan could also modulate the immune functions in aging model mice and the underlying mechanism.Methods: D-galactose (D-gal) is often used as an inducer of immunosenescence in animals. In this study, a mice model was created by subcutaneous D-gal (220 mg kg− 1) for successive 42 days. Then, the blood and spleen tissue samples were taken for the analysis and observation of cytokine levels, immunoglobulin levels, leukocyte numbers, and the phagocytic activity of macrophages, as well as the histological changes, the proliferation ability of lymphocytes, and the biochemical parameters in the spleen tissue.Results: Anwulignan significantly increased the serum levels of IL-2, IL-4, IFN-γ, lgG, lgM, and lgA, decreased the content of TNF-α and IL-6 in the aging mice, and increased the blood leukocyte number, the phagocytic activity, the lymphocyte proliferation, and the spleen index in vitro. Anwulignan also significantly increased the activities of SOD and GSH-Px, decreased the contents of MDA and 8-OHdG in the spleen tissue, up-regulated the expressions of Nrf2, HO-1, and Bcl2, down-regulated the expressions of Keap1, Caspase-3, and Bax in the spleen cells, and reduced the apoptosis of spleen lymphocytes.Conclusion: Anwulignan can restore the immune function that is declined in D-gal-induced aging mice partly related to its antioxidant capacity by activating the Nrf2/ARE pathway and downstream enzymes, as well as its anti-apoptotic effect by regulating Caspase-3 and the ratio of Bcl2 to Bax in the spleen.Keywords: Anwulignan, immunosenescence, antioxidation, anti-apoptosisLi XGao JYu ZJiang WSun WYu CSun JWang CChen JJing SLi HDove Medical Pressarticleanwulignanimmunosenescenceantioxidationanti-apoptosisGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 15, Pp 97-110 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	anwulignan immunosenescence antioxidation anti-apoptosis Geriatrics RC952-954.6
spellingShingle	anwulignan immunosenescence antioxidation anti-apoptosis Geriatrics RC952-954.6 Li X Gao J Yu Z Jiang W Sun W Yu C Sun J Wang C Chen J Jing S Li H Regulatory Effect of Anwulignan on the Immune Function Through Its Antioxidation and Anti-Apoptosis in D-Galactose-Induced Aging Mice
description	Xin Li,1 Jiaqi Gao,1 Zepeng Yu,1 Weihai Jiang,2 Wei Sun,2 Chunyan Yu,1 Jinghui Sun,1 Chunmei Wang,1 Jianguang Chen,1 Shu Jing,2 He Li1 1Department of Pharmacology, College of Pharmacy, Beihua University, Jilin, Jilin 132013, People’s Republic of China; 2Affiliated Hospital of Beihua University, Jilin, Jilin 132011, People’s Republic of ChinaCorrespondence: Shu Jing; He Li Email yitongjingshu@163.com; yitonglh@126.comBackground: Aging is a spontaneous and inevitable phenomenon of biology, which can lead to the gradual deterioration of tissues and organs. One of the age-related deterioration processes is immunosenescence, which leads to changes in the function of immune systems, including immune cells and associated cytokines. A proper modulation of immune responses can improve the age-related immunosenescence process and then reach healthy aging. Schisandra sphenanthera, a traditional Chinese medicine, has been used as both a medicine and a nutritional supplement for thousands of years. Anwulignan, a monomer compound of Schisandra sphenanthera lignans, has been reported to possess an immunomodulatory effect. Therefore, this study was designed to further explore whether Anwulignan could also modulate the immune functions in aging model mice and the underlying mechanism.Methods: D-galactose (D-gal) is often used as an inducer of immunosenescence in animals. In this study, a mice model was created by subcutaneous D-gal (220 mg kg− 1) for successive 42 days. Then, the blood and spleen tissue samples were taken for the analysis and observation of cytokine levels, immunoglobulin levels, leukocyte numbers, and the phagocytic activity of macrophages, as well as the histological changes, the proliferation ability of lymphocytes, and the biochemical parameters in the spleen tissue.Results: Anwulignan significantly increased the serum levels of IL-2, IL-4, IFN-γ, lgG, lgM, and lgA, decreased the content of TNF-α and IL-6 in the aging mice, and increased the blood leukocyte number, the phagocytic activity, the lymphocyte proliferation, and the spleen index in vitro. Anwulignan also significantly increased the activities of SOD and GSH-Px, decreased the contents of MDA and 8-OHdG in the spleen tissue, up-regulated the expressions of Nrf2, HO-1, and Bcl2, down-regulated the expressions of Keap1, Caspase-3, and Bax in the spleen cells, and reduced the apoptosis of spleen lymphocytes.Conclusion: Anwulignan can restore the immune function that is declined in D-gal-induced aging mice partly related to its antioxidant capacity by activating the Nrf2/ARE pathway and downstream enzymes, as well as its anti-apoptotic effect by regulating Caspase-3 and the ratio of Bcl2 to Bax in the spleen.Keywords: Anwulignan, immunosenescence, antioxidation, anti-apoptosis
format	article
author	Li X Gao J Yu Z Jiang W Sun W Yu C Sun J Wang C Chen J Jing S Li H
author_facet	Li X Gao J Yu Z Jiang W Sun W Yu C Sun J Wang C Chen J Jing S Li H
author_sort	Li X
title	Regulatory Effect of Anwulignan on the Immune Function Through Its Antioxidation and Anti-Apoptosis in D-Galactose-Induced Aging Mice
title_short	Regulatory Effect of Anwulignan on the Immune Function Through Its Antioxidation and Anti-Apoptosis in D-Galactose-Induced Aging Mice
title_full	Regulatory Effect of Anwulignan on the Immune Function Through Its Antioxidation and Anti-Apoptosis in D-Galactose-Induced Aging Mice
title_fullStr	Regulatory Effect of Anwulignan on the Immune Function Through Its Antioxidation and Anti-Apoptosis in D-Galactose-Induced Aging Mice
title_full_unstemmed	Regulatory Effect of Anwulignan on the Immune Function Through Its Antioxidation and Anti-Apoptosis in D-Galactose-Induced Aging Mice
title_sort	regulatory effect of anwulignan on the immune function through its antioxidation and anti-apoptosis in d-galactose-induced aging mice
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/6ec8c79bbaf34f1aa688838cc251c250
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Regulatory Effect of Anwulignan on the Immune Function Through Its Antioxidation and Anti-Apoptosis in D-Galactose-Induced Aging Mice

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