Optimal Extraction Process and In Vivo Anti-Inflammatory Evaluation of High Purity Oily Capsicum Oleoresin for Pharmaceutical Applications

Recently, plant-derived anti-inflammatory products have received an increasing attention from researchers due to their excellent in vivo activity with limited side effects. Therefore, the extraction of natural active compounds from the plant with high purity for use in anti-inflammatory formulations...

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Autores principales:	Dinh Tien Dung Nguyen, Mong Tham Vo, Cong Tri Truong, Dai Hai Nguyen, Thuy-Anh Nguyen Thi, Thanh Ngoc Huynh Truc, Nguyen Thanh Viet, Minh Hoang Vo Do
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/6ecbf1840f0042b382ec794f88329e5b
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spelling	oai:doaj.org-article:6ecbf1840f0042b382ec794f88329e5b2021-11-15T01:19:43ZOptimal Extraction Process and In Vivo Anti-Inflammatory Evaluation of High Purity Oily Capsicum Oleoresin for Pharmaceutical Applications1741-428810.1155/2021/8229607https://doaj.org/article/6ecbf1840f0042b382ec794f88329e5b2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/8229607https://doaj.org/toc/1741-4288Recently, plant-derived anti-inflammatory products have received an increasing attention from researchers due to their excellent in vivo activity with limited side effects. Therefore, the extraction of natural active compounds from the plant with high purity for use in anti-inflammatory formulations is required. In this study, oily Capsicum oleoresin (OCO) was extracted from Capsicum frutescens L. in ethanol by the ultrasound-assisted extraction technique, followed by a centrifugation step for a high purity OCO extract, which can be applied to develop anti-inflammatory formulations. The impact of various conditions (ethanol concentration, sonicating temperature, extraction time, solvent-to-sample ratio, and extraction repetition) on the efficiency of the extraction process was investigated. The results showed that the optimized conditions for the high yield of OCO were 95% ethanol, 50–60°C, 60 minutes, solvent-to-sample ratio of 5 : 1 ml/g, and one extraction repetition, followed by centrifuging at 5000 rpm in 2 hours. Then, the purity and in vivo anti-inflammatory activities of the obtained OCO was then determined by using the HPLC method and carrageenan-induced mice paw edema model, respectively. The purity of OCO was determined as 3.408 mg capsaicin per gram of Capsicum powder; meanwhile, its anti-inflammatory effect value was approximate to that of the commercial drug diclofenac after 48 hours of treatment. The high purity OCO prepared by this low-cost and ecofriendly extraction process would be a promising material for anti-inflammatory formulations.Dinh Tien Dung NguyenMong Tham VoCong Tri TruongDai Hai NguyenThuy-Anh Nguyen ThiThanh Ngoc Huynh TrucNguyen Thanh VietMinh Hoang Vo DoHindawi LimitedarticleOther systems of medicineRZ201-999ENEvidence-Based Complementary and Alternative Medicine, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Other systems of medicine RZ201-999
spellingShingle	Other systems of medicine RZ201-999 Dinh Tien Dung Nguyen Mong Tham Vo Cong Tri Truong Dai Hai Nguyen Thuy-Anh Nguyen Thi Thanh Ngoc Huynh Truc Nguyen Thanh Viet Minh Hoang Vo Do Optimal Extraction Process and In Vivo Anti-Inflammatory Evaluation of High Purity Oily Capsicum Oleoresin for Pharmaceutical Applications
description	Recently, plant-derived anti-inflammatory products have received an increasing attention from researchers due to their excellent in vivo activity with limited side effects. Therefore, the extraction of natural active compounds from the plant with high purity for use in anti-inflammatory formulations is required. In this study, oily Capsicum oleoresin (OCO) was extracted from Capsicum frutescens L. in ethanol by the ultrasound-assisted extraction technique, followed by a centrifugation step for a high purity OCO extract, which can be applied to develop anti-inflammatory formulations. The impact of various conditions (ethanol concentration, sonicating temperature, extraction time, solvent-to-sample ratio, and extraction repetition) on the efficiency of the extraction process was investigated. The results showed that the optimized conditions for the high yield of OCO were 95% ethanol, 50–60°C, 60 minutes, solvent-to-sample ratio of 5 : 1 ml/g, and one extraction repetition, followed by centrifuging at 5000 rpm in 2 hours. Then, the purity and in vivo anti-inflammatory activities of the obtained OCO was then determined by using the HPLC method and carrageenan-induced mice paw edema model, respectively. The purity of OCO was determined as 3.408 mg capsaicin per gram of Capsicum powder; meanwhile, its anti-inflammatory effect value was approximate to that of the commercial drug diclofenac after 48 hours of treatment. The high purity OCO prepared by this low-cost and ecofriendly extraction process would be a promising material for anti-inflammatory formulations.
format	article
author	Dinh Tien Dung Nguyen Mong Tham Vo Cong Tri Truong Dai Hai Nguyen Thuy-Anh Nguyen Thi Thanh Ngoc Huynh Truc Nguyen Thanh Viet Minh Hoang Vo Do
author_facet	Dinh Tien Dung Nguyen Mong Tham Vo Cong Tri Truong Dai Hai Nguyen Thuy-Anh Nguyen Thi Thanh Ngoc Huynh Truc Nguyen Thanh Viet Minh Hoang Vo Do
author_sort	Dinh Tien Dung Nguyen
title	Optimal Extraction Process and In Vivo Anti-Inflammatory Evaluation of High Purity Oily Capsicum Oleoresin for Pharmaceutical Applications
title_short	Optimal Extraction Process and In Vivo Anti-Inflammatory Evaluation of High Purity Oily Capsicum Oleoresin for Pharmaceutical Applications
title_full	Optimal Extraction Process and In Vivo Anti-Inflammatory Evaluation of High Purity Oily Capsicum Oleoresin for Pharmaceutical Applications
title_fullStr	Optimal Extraction Process and In Vivo Anti-Inflammatory Evaluation of High Purity Oily Capsicum Oleoresin for Pharmaceutical Applications
title_full_unstemmed	Optimal Extraction Process and In Vivo Anti-Inflammatory Evaluation of High Purity Oily Capsicum Oleoresin for Pharmaceutical Applications
title_sort	optimal extraction process and in vivo anti-inflammatory evaluation of high purity oily capsicum oleoresin for pharmaceutical applications
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/6ecbf1840f0042b382ec794f88329e5b
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Optimal Extraction Process and In Vivo Anti-Inflammatory Evaluation of High Purity Oily Capsicum Oleoresin for Pharmaceutical Applications

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