A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)

Abstract Background Rats have been widely used as experimental animals when performing fundamental research because they are economical, rapidly reproducing, and heal quickly. While the rat interbody fusion model has been applied in basic studies, existing rat models generally have shortcomings, suc...

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Autores principales:	Yu Kang, Chao Liu, Ming Wang, Cheng Wang, Yi-Guo Yan, Wen-Jun Wang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Animal model Interbody fusion Micro-CT Histology Diseases of the musculoskeletal system RC925-935
Acceso en línea:	https://doaj.org/article/6ecf565ef6924d36a8a36bc54bc32725
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spelling	oai:doaj.org-article:6ecf565ef6924d36a8a36bc54bc327252021-11-21T12:27:45ZA novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)10.1186/s12891-021-04822-41471-2474https://doaj.org/article/6ecf565ef6924d36a8a36bc54bc327252021-11-01T00:00:00Zhttps://doi.org/10.1186/s12891-021-04822-4https://doaj.org/toc/1471-2474Abstract Background Rats have been widely used as experimental animals when performing fundamental research because they are economical, rapidly reproducing, and heal quickly. While the rat interbody fusion model has been applied in basic studies, existing rat models generally have shortcomings, such as insufficiently simulating clinical surgery. The purpose of this study was to develop a novel rat model of interbody fusion which more closely represents clinical surgery. Methods The internal fixation was designed based on physical measurements of the rats’ lumbar spine. Then, ten rats divided into two groups (A and B) underwent anterior lumbar corpectomy and fusion of the L5 vertebrae. Groups A and B were sacrificed four and 8 weeks post-surgery, respectively. Micro-CT and histological examination were used to evaluate the model. Fusion rate, bone volume fraction (BV/TV), trabecular bone number (Tb.N), trabecular bone thickness (Tb.Th), and the area ratio of newly formed bone (NB) were calculated for quantitative analysis. Results Based on the L5 body dimensions of individual rats, 3D-printed titanium cage of the appropriate size were printed. The operations were successfully completed in all ten rats, and X-ray confirmed that internal fixation was good without migration. Micro-CT suggested that fusion rates in group B (100%) were greater than group A (40%, P < 0.05). The BV/TV (B: 42.20 ± 10.50 vs. A: 29.02 ± 3.25, P < 0.05) and Tb.N (B: 4.66 ± 1.23 vs. A: 1.97 ± 0.40, P < 0.05) were greater in group B than A, and the Tb.Th in group B was lower than group A (B: 0.10 ± 0.04 vs. A: 0.15 ± 0.02, P < 0.05). Histomorphometry results demonstrated that the area ratio of NB in group B were greater than group A (B: 35.72 ± 12.80 vs. A: 12.36 ± 16.93, P < 0.05). Conclusion A rat interbody fusion model based on anterior lumbar corpectomy and fusion has successfully been constructed and verified. It could provide a new choice for fundamental research using animal models of spinal fusion.Yu KangChao LiuMing WangCheng WangYi-Guo YanWen-Jun WangBMCarticleAnimal modelInterbody fusionMicro-CTHistologyDiseases of the musculoskeletal systemRC925-935ENBMC Musculoskeletal Disorders, Vol 22, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Animal model Interbody fusion Micro-CT Histology Diseases of the musculoskeletal system RC925-935
spellingShingle	Animal model Interbody fusion Micro-CT Histology Diseases of the musculoskeletal system RC925-935 Yu Kang Chao Liu Ming Wang Cheng Wang Yi-Guo Yan Wen-Jun Wang A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)
description	Abstract Background Rats have been widely used as experimental animals when performing fundamental research because they are economical, rapidly reproducing, and heal quickly. While the rat interbody fusion model has been applied in basic studies, existing rat models generally have shortcomings, such as insufficiently simulating clinical surgery. The purpose of this study was to develop a novel rat model of interbody fusion which more closely represents clinical surgery. Methods The internal fixation was designed based on physical measurements of the rats’ lumbar spine. Then, ten rats divided into two groups (A and B) underwent anterior lumbar corpectomy and fusion of the L5 vertebrae. Groups A and B were sacrificed four and 8 weeks post-surgery, respectively. Micro-CT and histological examination were used to evaluate the model. Fusion rate, bone volume fraction (BV/TV), trabecular bone number (Tb.N), trabecular bone thickness (Tb.Th), and the area ratio of newly formed bone (NB) were calculated for quantitative analysis. Results Based on the L5 body dimensions of individual rats, 3D-printed titanium cage of the appropriate size were printed. The operations were successfully completed in all ten rats, and X-ray confirmed that internal fixation was good without migration. Micro-CT suggested that fusion rates in group B (100%) were greater than group A (40%, P < 0.05). The BV/TV (B: 42.20 ± 10.50 vs. A: 29.02 ± 3.25, P < 0.05) and Tb.N (B: 4.66 ± 1.23 vs. A: 1.97 ± 0.40, P < 0.05) were greater in group B than A, and the Tb.Th in group B was lower than group A (B: 0.10 ± 0.04 vs. A: 0.15 ± 0.02, P < 0.05). Histomorphometry results demonstrated that the area ratio of NB in group B were greater than group A (B: 35.72 ± 12.80 vs. A: 12.36 ± 16.93, P < 0.05). Conclusion A rat interbody fusion model based on anterior lumbar corpectomy and fusion has successfully been constructed and verified. It could provide a new choice for fundamental research using animal models of spinal fusion.
format	article
author	Yu Kang Chao Liu Ming Wang Cheng Wang Yi-Guo Yan Wen-Jun Wang
author_facet	Yu Kang Chao Liu Ming Wang Cheng Wang Yi-Guo Yan Wen-Jun Wang
author_sort	Yu Kang
title	A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)
title_short	A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)
title_full	A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)
title_fullStr	A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)
title_full_unstemmed	A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)
title_sort	novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (alcf)
publisher	BMC
publishDate	2021
url	https://doaj.org/article/6ecf565ef6924d36a8a36bc54bc32725
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A novel rat model of interbody fusion based on anterior lumbar corpectomy and fusion (ALCF)

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