A portable microfluidic platform for rapid molecular diagnostic testing of patients with myeloproliferative neoplasms

Abstract The ability to simultaneously detect JAK2 V617F and MPL W515K/L mutations would substantially improve the early diagnosis of myeloproliferative neoplasms (MPNs) and decrease the risk of arterial thrombosis. The goal of this study is to achieve a point of care testing platform for simultaneo...

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Autores principales:	Hua Wang, Xinju zhang, Xiao Xu, Qunfeng Zhang, Hengliang Wang, Dong Li, Zhihua Kang, Zhiyuan Wu, Yigui Tang, Zhenhua An, Ming Guan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ed08cad26cf4bb38b1ecc0b63d2182a
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spelling	oai:doaj.org-article:6ed08cad26cf4bb38b1ecc0b63d2182a2021-12-02T16:06:22ZA portable microfluidic platform for rapid molecular diagnostic testing of patients with myeloproliferative neoplasms10.1038/s41598-017-08674-82045-2322https://doaj.org/article/6ed08cad26cf4bb38b1ecc0b63d2182a2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08674-8https://doaj.org/toc/2045-2322Abstract The ability to simultaneously detect JAK2 V617F and MPL W515K/L mutations would substantially improve the early diagnosis of myeloproliferative neoplasms (MPNs) and decrease the risk of arterial thrombosis. The goal of this study is to achieve a point of care testing platform for simultaneous analysis of major genetic alterations in MPN. Here, we report a microfluidic platform including a glass capillary containing polypropylene matrix that extracts genomic DNA from a drop of whole blood, a microchip for simultaneous multi-gene mutation screening, and a handheld battery-powered heating device. The µmLchip system was successfully used for point-of-care identification of the JAK2 V617F and MPL W515K/L mutations. The µmLchip assays were then validated by mutation analysis with samples from 100 MPN patients who had previously been analyzed via unlabeled probe melting curve analysis or real-time PCR. The results from the µmLchip were in perfect agreement with those from the other methods, except for one discrepant result that was negative in the unlabeled probe melting curve analysis but positive in the µmLchip. After T-A cloning, sequences of cloned PCR products revealed JAK2 V617F mutation in the sample. The portable microfluidic platform may be very attractive in developing point-of-care diagnostics for MPL W515K/L and JAK2 V617F mutations.Hua WangXinju zhangXiao XuQunfeng ZhangHengliang WangDong LiZhihua KangZhiyuan WuYigui TangZhenhua AnMing GuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hua Wang Xinju zhang Xiao Xu Qunfeng Zhang Hengliang Wang Dong Li Zhihua Kang Zhiyuan Wu Yigui Tang Zhenhua An Ming Guan A portable microfluidic platform for rapid molecular diagnostic testing of patients with myeloproliferative neoplasms
description	Abstract The ability to simultaneously detect JAK2 V617F and MPL W515K/L mutations would substantially improve the early diagnosis of myeloproliferative neoplasms (MPNs) and decrease the risk of arterial thrombosis. The goal of this study is to achieve a point of care testing platform for simultaneous analysis of major genetic alterations in MPN. Here, we report a microfluidic platform including a glass capillary containing polypropylene matrix that extracts genomic DNA from a drop of whole blood, a microchip for simultaneous multi-gene mutation screening, and a handheld battery-powered heating device. The µmLchip system was successfully used for point-of-care identification of the JAK2 V617F and MPL W515K/L mutations. The µmLchip assays were then validated by mutation analysis with samples from 100 MPN patients who had previously been analyzed via unlabeled probe melting curve analysis or real-time PCR. The results from the µmLchip were in perfect agreement with those from the other methods, except for one discrepant result that was negative in the unlabeled probe melting curve analysis but positive in the µmLchip. After T-A cloning, sequences of cloned PCR products revealed JAK2 V617F mutation in the sample. The portable microfluidic platform may be very attractive in developing point-of-care diagnostics for MPL W515K/L and JAK2 V617F mutations.
format	article
author	Hua Wang Xinju zhang Xiao Xu Qunfeng Zhang Hengliang Wang Dong Li Zhihua Kang Zhiyuan Wu Yigui Tang Zhenhua An Ming Guan
author_facet	Hua Wang Xinju zhang Xiao Xu Qunfeng Zhang Hengliang Wang Dong Li Zhihua Kang Zhiyuan Wu Yigui Tang Zhenhua An Ming Guan
author_sort	Hua Wang
title	A portable microfluidic platform for rapid molecular diagnostic testing of patients with myeloproliferative neoplasms
title_short	A portable microfluidic platform for rapid molecular diagnostic testing of patients with myeloproliferative neoplasms
title_full	A portable microfluidic platform for rapid molecular diagnostic testing of patients with myeloproliferative neoplasms
title_fullStr	A portable microfluidic platform for rapid molecular diagnostic testing of patients with myeloproliferative neoplasms
title_full_unstemmed	A portable microfluidic platform for rapid molecular diagnostic testing of patients with myeloproliferative neoplasms
title_sort	portable microfluidic platform for rapid molecular diagnostic testing of patients with myeloproliferative neoplasms
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6ed08cad26cf4bb38b1ecc0b63d2182a
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A portable microfluidic platform for rapid molecular diagnostic testing of patients with myeloproliferative neoplasms

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