Disassembling the Nature of Capsid: Biochemical, Genetic, and Imaging Approaches to Assess HIV-1 Capsid Functions

The human immunodeficiency virus type 1 (HIV-1) capsid and its disassembly, or capsid uncoating, has remained an active area of study over the past several decades. Our understanding of the HIV-1 capsid as solely a protective shell has since shifted with discoveries linking it to other complex repli...

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Autores principales:	Zachary Ingram, Douglas K. Fischer, Zandrea Ambrose
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	HIV-1 capsid trafficking nuclear import reverse transcription uncoating Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/6ed1da04856a4e8b8ec6a348399e943a
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spelling	oai:doaj.org-article:6ed1da04856a4e8b8ec6a348399e943a2021-11-25T19:13:38ZDisassembling the Nature of Capsid: Biochemical, Genetic, and Imaging Approaches to Assess HIV-1 Capsid Functions10.3390/v131122371999-4915https://doaj.org/article/6ed1da04856a4e8b8ec6a348399e943a2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2237https://doaj.org/toc/1999-4915The human immunodeficiency virus type 1 (HIV-1) capsid and its disassembly, or capsid uncoating, has remained an active area of study over the past several decades. Our understanding of the HIV-1 capsid as solely a protective shell has since shifted with discoveries linking it to other complex replication events. The interplay of the HIV-1 capsid with reverse transcription, nuclear import, and integration has led to an expansion of knowledge of capsid functionality. Coincident with advances in microscopy, cell, and biochemistry assays, several models of capsid disassembly have been proposed, in which it occurs in either the cytoplasmic, nuclear envelope, or nuclear regions of the cell. Here, we discuss how the understanding of the HIV-1 capsid has evolved and the key methods that made these discoveries possible.Zachary IngramDouglas K. FischerZandrea AmbroseMDPI AGarticleHIV-1capsidtraffickingnuclear importreverse transcriptionuncoatingMicrobiologyQR1-502ENViruses, Vol 13, Iss 2237, p 2237 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HIV-1 capsid trafficking nuclear import reverse transcription uncoating Microbiology QR1-502
spellingShingle	HIV-1 capsid trafficking nuclear import reverse transcription uncoating Microbiology QR1-502 Zachary Ingram Douglas K. Fischer Zandrea Ambrose Disassembling the Nature of Capsid: Biochemical, Genetic, and Imaging Approaches to Assess HIV-1 Capsid Functions
description	The human immunodeficiency virus type 1 (HIV-1) capsid and its disassembly, or capsid uncoating, has remained an active area of study over the past several decades. Our understanding of the HIV-1 capsid as solely a protective shell has since shifted with discoveries linking it to other complex replication events. The interplay of the HIV-1 capsid with reverse transcription, nuclear import, and integration has led to an expansion of knowledge of capsid functionality. Coincident with advances in microscopy, cell, and biochemistry assays, several models of capsid disassembly have been proposed, in which it occurs in either the cytoplasmic, nuclear envelope, or nuclear regions of the cell. Here, we discuss how the understanding of the HIV-1 capsid has evolved and the key methods that made these discoveries possible.
format	article
author	Zachary Ingram Douglas K. Fischer Zandrea Ambrose
author_facet	Zachary Ingram Douglas K. Fischer Zandrea Ambrose
author_sort	Zachary Ingram
title	Disassembling the Nature of Capsid: Biochemical, Genetic, and Imaging Approaches to Assess HIV-1 Capsid Functions
title_short	Disassembling the Nature of Capsid: Biochemical, Genetic, and Imaging Approaches to Assess HIV-1 Capsid Functions
title_full	Disassembling the Nature of Capsid: Biochemical, Genetic, and Imaging Approaches to Assess HIV-1 Capsid Functions
title_fullStr	Disassembling the Nature of Capsid: Biochemical, Genetic, and Imaging Approaches to Assess HIV-1 Capsid Functions
title_full_unstemmed	Disassembling the Nature of Capsid: Biochemical, Genetic, and Imaging Approaches to Assess HIV-1 Capsid Functions
title_sort	disassembling the nature of capsid: biochemical, genetic, and imaging approaches to assess hiv-1 capsid functions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6ed1da04856a4e8b8ec6a348399e943a
work_keys_str_mv	AT zacharyingram disassemblingthenatureofcapsidbiochemicalgeneticandimagingapproachestoassesshiv1capsidfunctions AT douglaskfischer disassemblingthenatureofcapsidbiochemicalgeneticandimagingapproachestoassesshiv1capsidfunctions AT zandreaambrose disassemblingthenatureofcapsidbiochemicalgeneticandimagingapproachestoassesshiv1capsidfunctions
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Disassembling the Nature of Capsid: Biochemical, Genetic, and Imaging Approaches to Assess HIV-1 Capsid Functions

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