Shape dependent cytotoxicity of PLGA-PEG nanoparticles on human cells

Abstract We investigated the influence of nanoparticles’ shape on the physiological responses of cells, when they were fed with spherical and needle-shaped PLGA-PEG nanoparticles (the volume of the nanoparticles had been chosen as the fixed parameter). We found that both types of NPs entered cells v...

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Autores principales:	Bokai Zhang, Ping Sai Lung, Saisai Zhao, Zhiqin Chu, Wojciech Chrzanowski, Quan Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ed2ac9724064576879402e9b41b2596
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spelling	oai:doaj.org-article:6ed2ac9724064576879402e9b41b25962021-12-02T12:32:31ZShape dependent cytotoxicity of PLGA-PEG nanoparticles on human cells10.1038/s41598-017-07588-92045-2322https://doaj.org/article/6ed2ac9724064576879402e9b41b25962017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07588-9https://doaj.org/toc/2045-2322Abstract We investigated the influence of nanoparticles’ shape on the physiological responses of cells, when they were fed with spherical and needle-shaped PLGA-PEG nanoparticles (the volume of the nanoparticles had been chosen as the fixed parameter). We found that both types of NPs entered cells via endocytosis and upon internalization they stayed in membrane bounded vesicles. Needle-shaped, but not the spherical-shaped NPs were found to induce significant cytotoxicity in the cell lines tested. Our study evidenced that the cytotoxicity of needle-shaped NPs was induced through the lysosome disruption. Lysosome damage activated the signaling pathways for cell apoptosis, and eventually caused DNA fragmentation and cell death. The present work showed that physiological response of the cells can be very different when the shape of the fed nanoparticles changed from spherical to needle-like. The finding suggests that the toxicity of nanomaterials also depends on their shape.Bokai ZhangPing Sai LungSaisai ZhaoZhiqin ChuWojciech ChrzanowskiQuan LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bokai Zhang Ping Sai Lung Saisai Zhao Zhiqin Chu Wojciech Chrzanowski Quan Li Shape dependent cytotoxicity of PLGA-PEG nanoparticles on human cells
description	Abstract We investigated the influence of nanoparticles’ shape on the physiological responses of cells, when they were fed with spherical and needle-shaped PLGA-PEG nanoparticles (the volume of the nanoparticles had been chosen as the fixed parameter). We found that both types of NPs entered cells via endocytosis and upon internalization they stayed in membrane bounded vesicles. Needle-shaped, but not the spherical-shaped NPs were found to induce significant cytotoxicity in the cell lines tested. Our study evidenced that the cytotoxicity of needle-shaped NPs was induced through the lysosome disruption. Lysosome damage activated the signaling pathways for cell apoptosis, and eventually caused DNA fragmentation and cell death. The present work showed that physiological response of the cells can be very different when the shape of the fed nanoparticles changed from spherical to needle-like. The finding suggests that the toxicity of nanomaterials also depends on their shape.
format	article
author	Bokai Zhang Ping Sai Lung Saisai Zhao Zhiqin Chu Wojciech Chrzanowski Quan Li
author_facet	Bokai Zhang Ping Sai Lung Saisai Zhao Zhiqin Chu Wojciech Chrzanowski Quan Li
author_sort	Bokai Zhang
title	Shape dependent cytotoxicity of PLGA-PEG nanoparticles on human cells
title_short	Shape dependent cytotoxicity of PLGA-PEG nanoparticles on human cells
title_full	Shape dependent cytotoxicity of PLGA-PEG nanoparticles on human cells
title_fullStr	Shape dependent cytotoxicity of PLGA-PEG nanoparticles on human cells
title_full_unstemmed	Shape dependent cytotoxicity of PLGA-PEG nanoparticles on human cells
title_sort	shape dependent cytotoxicity of plga-peg nanoparticles on human cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6ed2ac9724064576879402e9b41b2596
work_keys_str_mv	AT bokaizhang shapedependentcytotoxicityofplgapegnanoparticlesonhumancells AT pingsailung shapedependentcytotoxicityofplgapegnanoparticlesonhumancells AT saisaizhao shapedependentcytotoxicityofplgapegnanoparticlesonhumancells AT zhiqinchu shapedependentcytotoxicityofplgapegnanoparticlesonhumancells AT wojciechchrzanowski shapedependentcytotoxicityofplgapegnanoparticlesonhumancells AT quanli shapedependentcytotoxicityofplgapegnanoparticlesonhumancells
_version_	1718394039400660992

Shape dependent cytotoxicity of PLGA-PEG nanoparticles on human cells

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