A MULTIPLEX ASSAY OF 26 CYTOKINES SECRETED BY BLOOD CELLS OF PATIENTS WITH LIVER CIRRHOSIS

Cytokines play an important role in the pathogenesis of liver cirrhosis (LC), determining the disease severity as well as hepatic and extrahepatic complications. The present study aims to characterize the cytokine profile of blood cells in 20 LC patients with regard to the stage (class A on Child-Pu...

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Autores principales:	A. A. Ostanin, N. M. Starostina, I. V. Meledina, M. V. Shipunov, O. Yu. Leplina, E. Ya. Shevela, E. R. Chernykh
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2016
Materias:	multiplex assay cytokines chemokines growth factors blood cells liver cirrhosis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/6ed57ef3368f4ab492df059a07b6ee0a
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spelling	oai:doaj.org-article:6ed57ef3368f4ab492df059a07b6ee0a2021-11-18T08:03:45ZA MULTIPLEX ASSAY OF 26 CYTOKINES SECRETED BY BLOOD CELLS OF PATIENTS WITH LIVER CIRRHOSIS1563-06252313-741X10.15789/1563-0625-2015-6-539-552https://doaj.org/article/6ed57ef3368f4ab492df059a07b6ee0a2016-01-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/961https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XCytokines play an important role in the pathogenesis of liver cirrhosis (LC), determining the disease severity as well as hepatic and extrahepatic complications. The present study aims to characterize the cytokine profile of blood cells in 20 LC patients with regard to the stage (class A on Child-Pugh, n = 13, and the class B+C, n = 7) and the etiology of the disease (viral LC, n = 12 and the LC of non-viral etiology, n = 8) using cytokine 26-plex assay. The control group consisted of 10 healthy donors. Cytokine concentrations were tested both in spontaneous and bacterial endotoxin (LPS) stimulated 24 hour blood cultures. Four functional groups: pro-/anti-inflammatory cytokines (IL-1β, TNFα, IL-1ra, IL-10), immunoregulatory cytokines (IL-2, IFNγ, IL-12, IL-4, IL-5, IL-6, IL-9, IL-13, IL-15, IL-17), growth factors (G-CSF, IL-7, FGF-β, PDGF, VEGF) and chemokines (IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, Eotaxin) involved in the pathogenesis of inflammation, have been analyzed. The blood cells of LC patients were shown to be active producers of cytokines and were characterized by increased cytokine levels from all four functional groups. The enhancement of many cytokines was associated with the disease severity and viral etiology. The increased cytokine production in both intact and LPS-stimulated cultures indicated preserved reactivity of blood cells to endotoxin. Overall, the data obtained suggest the contribution of circulating blood cells in supporting of inflammatory response and the potential diagnostic/prognostic significance of blood cell-derived cytokines in patients with LC.A. A. OstaninN. M. StarostinaI. V. MeledinaM. V. ShipunovO. Yu. LeplinaE. Ya. ShevelaE. R. ChernykhSPb RAACIarticlemultiplex assaycytokineschemokinesgrowth factorsblood cellsliver cirrhosisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 17, Iss 6, Pp 539-552 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	multiplex assay cytokines chemokines growth factors blood cells liver cirrhosis Immunologic diseases. Allergy RC581-607
spellingShingle	multiplex assay cytokines chemokines growth factors blood cells liver cirrhosis Immunologic diseases. Allergy RC581-607 A. A. Ostanin N. M. Starostina I. V. Meledina M. V. Shipunov O. Yu. Leplina E. Ya. Shevela E. R. Chernykh A MULTIPLEX ASSAY OF 26 CYTOKINES SECRETED BY BLOOD CELLS OF PATIENTS WITH LIVER CIRRHOSIS
description	Cytokines play an important role in the pathogenesis of liver cirrhosis (LC), determining the disease severity as well as hepatic and extrahepatic complications. The present study aims to characterize the cytokine profile of blood cells in 20 LC patients with regard to the stage (class A on Child-Pugh, n = 13, and the class B+C, n = 7) and the etiology of the disease (viral LC, n = 12 and the LC of non-viral etiology, n = 8) using cytokine 26-plex assay. The control group consisted of 10 healthy donors. Cytokine concentrations were tested both in spontaneous and bacterial endotoxin (LPS) stimulated 24 hour blood cultures. Four functional groups: pro-/anti-inflammatory cytokines (IL-1β, TNFα, IL-1ra, IL-10), immunoregulatory cytokines (IL-2, IFNγ, IL-12, IL-4, IL-5, IL-6, IL-9, IL-13, IL-15, IL-17), growth factors (G-CSF, IL-7, FGF-β, PDGF, VEGF) and chemokines (IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, RANTES, Eotaxin) involved in the pathogenesis of inflammation, have been analyzed. The blood cells of LC patients were shown to be active producers of cytokines and were characterized by increased cytokine levels from all four functional groups. The enhancement of many cytokines was associated with the disease severity and viral etiology. The increased cytokine production in both intact and LPS-stimulated cultures indicated preserved reactivity of blood cells to endotoxin. Overall, the data obtained suggest the contribution of circulating blood cells in supporting of inflammatory response and the potential diagnostic/prognostic significance of blood cell-derived cytokines in patients with LC.
format	article
author	A. A. Ostanin N. M. Starostina I. V. Meledina M. V. Shipunov O. Yu. Leplina E. Ya. Shevela E. R. Chernykh
author_facet	A. A. Ostanin N. M. Starostina I. V. Meledina M. V. Shipunov O. Yu. Leplina E. Ya. Shevela E. R. Chernykh
author_sort	A. A. Ostanin
title	A MULTIPLEX ASSAY OF 26 CYTOKINES SECRETED BY BLOOD CELLS OF PATIENTS WITH LIVER CIRRHOSIS
title_short	A MULTIPLEX ASSAY OF 26 CYTOKINES SECRETED BY BLOOD CELLS OF PATIENTS WITH LIVER CIRRHOSIS
title_full	A MULTIPLEX ASSAY OF 26 CYTOKINES SECRETED BY BLOOD CELLS OF PATIENTS WITH LIVER CIRRHOSIS
title_fullStr	A MULTIPLEX ASSAY OF 26 CYTOKINES SECRETED BY BLOOD CELLS OF PATIENTS WITH LIVER CIRRHOSIS
title_full_unstemmed	A MULTIPLEX ASSAY OF 26 CYTOKINES SECRETED BY BLOOD CELLS OF PATIENTS WITH LIVER CIRRHOSIS
title_sort	multiplex assay of 26 cytokines secreted by blood cells of patients with liver cirrhosis
publisher	SPb RAACI
publishDate	2016
url	https://doaj.org/article/6ed57ef3368f4ab492df059a07b6ee0a
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A MULTIPLEX ASSAY OF 26 CYTOKINES SECRETED BY BLOOD CELLS OF PATIENTS WITH LIVER CIRRHOSIS

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