Efficient genetic transformation method for Eucalyptus genome editing.

Plantation forestry of Eucalyptus urophylla × Eucalyptus grandis supplies high-quality raw material for pulp, paper, wood, and energy and thereby reduces the pressures on native forests and their associated biodiversity. Nevertheless, owing to the heterozygosity of the E. urophylla × E. grandis gene...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zechen Wang, Limei Li, Lejun Ouyang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ed65da88d8b49018aa3f013fc902116
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6ed65da88d8b49018aa3f013fc902116
record_format	dspace
spelling	oai:doaj.org-article:6ed65da88d8b49018aa3f013fc9021162021-11-25T06:19:03ZEfficient genetic transformation method for Eucalyptus genome editing.1932-620310.1371/journal.pone.0252011https://doaj.org/article/6ed65da88d8b49018aa3f013fc9021162021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252011https://doaj.org/toc/1932-6203Plantation forestry of Eucalyptus urophylla × Eucalyptus grandis supplies high-quality raw material for pulp, paper, wood, and energy and thereby reduces the pressures on native forests and their associated biodiversity. Nevertheless, owing to the heterozygosity of the E. urophylla × E. grandis genetic background, germplasm improvement by crossbreeding tends to be inefficient. As an alternative approach, genetic engineering of Eucalyptus can be used to effectively improve germplasm resources. From a strategic standpoint, increasing the plantation productivity and wood quality by transgenic technology has become increasingly important for forest industry. In this study, we established a fluorescence labelling method using CRISPR/Cas9 technology to obtain positive transformed progenies. The positive transformed progenies were easily obtained from the genetically modified population via fluorescence screening. This system can be used as a plant genome site-specific editing tool and may be useful for improving Eucalyptus genetic resources.Zechen WangLimei LiLejun OuyangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0252011 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zechen Wang Limei Li Lejun Ouyang Efficient genetic transformation method for Eucalyptus genome editing.
description	Plantation forestry of Eucalyptus urophylla × Eucalyptus grandis supplies high-quality raw material for pulp, paper, wood, and energy and thereby reduces the pressures on native forests and their associated biodiversity. Nevertheless, owing to the heterozygosity of the E. urophylla × E. grandis genetic background, germplasm improvement by crossbreeding tends to be inefficient. As an alternative approach, genetic engineering of Eucalyptus can be used to effectively improve germplasm resources. From a strategic standpoint, increasing the plantation productivity and wood quality by transgenic technology has become increasingly important for forest industry. In this study, we established a fluorescence labelling method using CRISPR/Cas9 technology to obtain positive transformed progenies. The positive transformed progenies were easily obtained from the genetically modified population via fluorescence screening. This system can be used as a plant genome site-specific editing tool and may be useful for improving Eucalyptus genetic resources.
format	article
author	Zechen Wang Limei Li Lejun Ouyang
author_facet	Zechen Wang Limei Li Lejun Ouyang
author_sort	Zechen Wang
title	Efficient genetic transformation method for Eucalyptus genome editing.
title_short	Efficient genetic transformation method for Eucalyptus genome editing.
title_full	Efficient genetic transformation method for Eucalyptus genome editing.
title_fullStr	Efficient genetic transformation method for Eucalyptus genome editing.
title_full_unstemmed	Efficient genetic transformation method for Eucalyptus genome editing.
title_sort	efficient genetic transformation method for eucalyptus genome editing.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6ed65da88d8b49018aa3f013fc902116
work_keys_str_mv	AT zechenwang efficientgenetictransformationmethodforeucalyptusgenomeediting AT limeili efficientgenetictransformationmethodforeucalyptusgenomeediting AT lejunouyang efficientgenetictransformationmethodforeucalyptusgenomeediting
_version_	1718413929991897088

Efficient genetic transformation method for Eucalyptus genome editing.

Ejemplares similares