Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain
Vitamin D<sub>3</sub> hypovitaminosis is associated with several neurological diseases such as Alzheimer’s disease, Parkinson’s disease or multiple sclerosis but also with other diseases such as cancer, diabetes or diseases linked to inflammatory processes. Importantly, in all of these d...
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2021
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oai:doaj.org-article:6ed9758e9c8c4e8cb7de18f91a42be342021-11-25T16:53:56ZImpact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain10.3390/biom111116992218-273Xhttps://doaj.org/article/6ed9758e9c8c4e8cb7de18f91a42be342021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1699https://doaj.org/toc/2218-273XVitamin D<sub>3</sub> hypovitaminosis is associated with several neurological diseases such as Alzheimer’s disease, Parkinson’s disease or multiple sclerosis but also with other diseases such as cancer, diabetes or diseases linked to inflammatory processes. Importantly, in all of these diseases lipids have at least a disease modifying effect. Besides its well-known property to modulate gene-expression via the VDR-receptor, less is known if vitamin D hypovitaminosis influences lipid homeostasis and if these potential changes contribute to the pathology of the diseases themselves. Therefore, we analyzed mouse brain with a mild vitamin D hypovitaminosis via a targeted shotgun lipidomic approach, including phosphatidylcholine, plasmalogens, lyso-phosphatidylcholine, (acyl-/acetyl-) carnitines and triglycerides. Alterations were compared with neuroblastoma cells cultivated in the presence and with decreased levels of vitamin D. Both in cell culture and in vivo, decreased vitamin D level resulted in changed lipid levels. While triglycerides were decreased, carnitines were increased under vitamin D hypovitaminosis suggesting an impact of vitamin D on energy metabolism. Additionally, lyso-phosphatidylcholines in particular saturated phosphatidylcholine (e.g., PC aa 48:0) and plasmalogen species (e.g., PC ae 42:0) tended to be increased. Our results suggest that vitamin D hypovitaminosis not only may affect gene expression but also may directly influence cellular lipid homeostasis and affect lipid turnover in disease states that are known for vitamin D hypovitaminosis.Anna Andrea LauerLea Victoria GriebschSabrina Melanie PilzDaniel JanitschkeElena Leoni TheissJörg ReichrathChristian HerrChristoph BeisswengerRobert BalsTeresa Giovanna ValencakDorothea PortiusHeike Sabine GrimmTobias HartmannMarcus Otto Walter GrimmMDPI AGarticlevitamin D hypovitaminosisshotgun lipidomicscalcitriolneurodegenerative diseasesphosphatidylcholinephosphatidylcholine plasmalogenMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1699, p 1699 (2021) |
| institution |
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| collection |
DOAJ |
| language |
EN |
| topic |
vitamin D hypovitaminosis shotgun lipidomics calcitriol neurodegenerative diseases phosphatidylcholine phosphatidylcholine plasmalogen Microbiology QR1-502 |
| spellingShingle |
vitamin D hypovitaminosis shotgun lipidomics calcitriol neurodegenerative diseases phosphatidylcholine phosphatidylcholine plasmalogen Microbiology QR1-502 Anna Andrea Lauer Lea Victoria Griebsch Sabrina Melanie Pilz Daniel Janitschke Elena Leoni Theiss Jörg Reichrath Christian Herr Christoph Beisswenger Robert Bals Teresa Giovanna Valencak Dorothea Portius Heike Sabine Grimm Tobias Hartmann Marcus Otto Walter Grimm Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain |
| description |
Vitamin D<sub>3</sub> hypovitaminosis is associated with several neurological diseases such as Alzheimer’s disease, Parkinson’s disease or multiple sclerosis but also with other diseases such as cancer, diabetes or diseases linked to inflammatory processes. Importantly, in all of these diseases lipids have at least a disease modifying effect. Besides its well-known property to modulate gene-expression via the VDR-receptor, less is known if vitamin D hypovitaminosis influences lipid homeostasis and if these potential changes contribute to the pathology of the diseases themselves. Therefore, we analyzed mouse brain with a mild vitamin D hypovitaminosis via a targeted shotgun lipidomic approach, including phosphatidylcholine, plasmalogens, lyso-phosphatidylcholine, (acyl-/acetyl-) carnitines and triglycerides. Alterations were compared with neuroblastoma cells cultivated in the presence and with decreased levels of vitamin D. Both in cell culture and in vivo, decreased vitamin D level resulted in changed lipid levels. While triglycerides were decreased, carnitines were increased under vitamin D hypovitaminosis suggesting an impact of vitamin D on energy metabolism. Additionally, lyso-phosphatidylcholines in particular saturated phosphatidylcholine (e.g., PC aa 48:0) and plasmalogen species (e.g., PC ae 42:0) tended to be increased. Our results suggest that vitamin D hypovitaminosis not only may affect gene expression but also may directly influence cellular lipid homeostasis and affect lipid turnover in disease states that are known for vitamin D hypovitaminosis. |
| format |
article |
| author |
Anna Andrea Lauer Lea Victoria Griebsch Sabrina Melanie Pilz Daniel Janitschke Elena Leoni Theiss Jörg Reichrath Christian Herr Christoph Beisswenger Robert Bals Teresa Giovanna Valencak Dorothea Portius Heike Sabine Grimm Tobias Hartmann Marcus Otto Walter Grimm |
| author_facet |
Anna Andrea Lauer Lea Victoria Griebsch Sabrina Melanie Pilz Daniel Janitschke Elena Leoni Theiss Jörg Reichrath Christian Herr Christoph Beisswenger Robert Bals Teresa Giovanna Valencak Dorothea Portius Heike Sabine Grimm Tobias Hartmann Marcus Otto Walter Grimm |
| author_sort |
Anna Andrea Lauer |
| title |
Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain |
| title_short |
Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain |
| title_full |
Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain |
| title_fullStr |
Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain |
| title_full_unstemmed |
Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain |
| title_sort |
impact of vitamin d<sub>3</sub> deficiency on phosphatidylcholine-/ethanolamine, plasmalogen-, lyso-phosphatidylcholine-/ethanolamine, carnitine- and triacyl glyceride-homeostasis in neuroblastoma cells and murine brain |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/6ed9758e9c8c4e8cb7de18f91a42be34 |
| work_keys_str_mv |
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