Zika-exposed microcephalic neonates exhibit higher degree of inflammatory imbalance in cerebrospinal fluid

Abstract Not every neonate with congenital Zika virus (ZIKV) infection (CZI) is born with microcephaly. We compared inflammation mediators in CSF (cerebrospinal fluid obtained from lumbar puncture) between ZIKV-exposed neonates with/without microcephaly (cases) and controls. In Brazil, in the same l...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gustavo C. Nascimento-Carvalho, Eduardo C. Nascimento-Carvalho, Clara L. Ramos, Ana-Luisa Vilas-Boas, Otávio A. Moreno-Carvalho, Caian L. Vinhaes, Beatriz Barreto-Duarte, Artur T. L. Queiroz, Bruno B. Andrade, Cristiana M. Nascimento-Carvalho
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6edef74aae5a4a818c4383510cc58623
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6edef74aae5a4a818c4383510cc58623
record_format	dspace
spelling	oai:doaj.org-article:6edef74aae5a4a818c4383510cc586232021-12-02T17:32:58ZZika-exposed microcephalic neonates exhibit higher degree of inflammatory imbalance in cerebrospinal fluid10.1038/s41598-021-87895-42045-2322https://doaj.org/article/6edef74aae5a4a818c4383510cc586232021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87895-4https://doaj.org/toc/2045-2322Abstract Not every neonate with congenital Zika virus (ZIKV) infection (CZI) is born with microcephaly. We compared inflammation mediators in CSF (cerebrospinal fluid obtained from lumbar puncture) between ZIKV-exposed neonates with/without microcephaly (cases) and controls. In Brazil, in the same laboratory, we identified 14 ZIKV-exposed neonates during the ZIKV epidemic (2015–2016), 7(50%) with and 7(50%) without microcephaly, without any other congenital infection, and 14 neonates (2017–2018) eligible to be controls and to match cases. 29 inflammation mediators were measured using Luminex immunoassay and multidimensional analyses were employed. Neonates with ZIKV-associated microcephaly presented substantially higher degree of inflammatory perturbation, associated with uncoupled inflammatory response and decreased correlations between concentrations of inflammatory biomarkers. The groups of microcephalic and non-microcephalic ZIKV-exposed neonates were distinguished from the control group (area under curve [AUC] = 1; P < 0.0001). Between controls and those non-microcephalic exposed to ZIKV, IL-1β, IL-3, IL-4, IL-7 and EOTAXIN were the top CSF markers. By comparing the microcephalic cases with controls, the top discriminant scores were for IL-1β, IL-3, EOTAXIN and IL-12p70. The degree of inflammatory imbalance may be associated with microcephaly in CZI and it may aid additional investigations in experimental pre-clinical models testing immune modulators in preventing extensive damage of the Central Nervous System.Gustavo C. Nascimento-CarvalhoEduardo C. Nascimento-CarvalhoClara L. RamosAna-Luisa Vilas-BoasOtávio A. Moreno-CarvalhoCaian L. VinhaesBeatriz Barreto-DuarteArtur T. L. QueirozBruno B. AndradeCristiana M. Nascimento-CarvalhoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gustavo C. Nascimento-Carvalho Eduardo C. Nascimento-Carvalho Clara L. Ramos Ana-Luisa Vilas-Boas Otávio A. Moreno-Carvalho Caian L. Vinhaes Beatriz Barreto-Duarte Artur T. L. Queiroz Bruno B. Andrade Cristiana M. Nascimento-Carvalho Zika-exposed microcephalic neonates exhibit higher degree of inflammatory imbalance in cerebrospinal fluid
description	Abstract Not every neonate with congenital Zika virus (ZIKV) infection (CZI) is born with microcephaly. We compared inflammation mediators in CSF (cerebrospinal fluid obtained from lumbar puncture) between ZIKV-exposed neonates with/without microcephaly (cases) and controls. In Brazil, in the same laboratory, we identified 14 ZIKV-exposed neonates during the ZIKV epidemic (2015–2016), 7(50%) with and 7(50%) without microcephaly, without any other congenital infection, and 14 neonates (2017–2018) eligible to be controls and to match cases. 29 inflammation mediators were measured using Luminex immunoassay and multidimensional analyses were employed. Neonates with ZIKV-associated microcephaly presented substantially higher degree of inflammatory perturbation, associated with uncoupled inflammatory response and decreased correlations between concentrations of inflammatory biomarkers. The groups of microcephalic and non-microcephalic ZIKV-exposed neonates were distinguished from the control group (area under curve [AUC] = 1; P < 0.0001). Between controls and those non-microcephalic exposed to ZIKV, IL-1β, IL-3, IL-4, IL-7 and EOTAXIN were the top CSF markers. By comparing the microcephalic cases with controls, the top discriminant scores were for IL-1β, IL-3, EOTAXIN and IL-12p70. The degree of inflammatory imbalance may be associated with microcephaly in CZI and it may aid additional investigations in experimental pre-clinical models testing immune modulators in preventing extensive damage of the Central Nervous System.
format	article
author	Gustavo C. Nascimento-Carvalho Eduardo C. Nascimento-Carvalho Clara L. Ramos Ana-Luisa Vilas-Boas Otávio A. Moreno-Carvalho Caian L. Vinhaes Beatriz Barreto-Duarte Artur T. L. Queiroz Bruno B. Andrade Cristiana M. Nascimento-Carvalho
author_facet	Gustavo C. Nascimento-Carvalho Eduardo C. Nascimento-Carvalho Clara L. Ramos Ana-Luisa Vilas-Boas Otávio A. Moreno-Carvalho Caian L. Vinhaes Beatriz Barreto-Duarte Artur T. L. Queiroz Bruno B. Andrade Cristiana M. Nascimento-Carvalho
author_sort	Gustavo C. Nascimento-Carvalho
title	Zika-exposed microcephalic neonates exhibit higher degree of inflammatory imbalance in cerebrospinal fluid
title_short	Zika-exposed microcephalic neonates exhibit higher degree of inflammatory imbalance in cerebrospinal fluid
title_full	Zika-exposed microcephalic neonates exhibit higher degree of inflammatory imbalance in cerebrospinal fluid
title_fullStr	Zika-exposed microcephalic neonates exhibit higher degree of inflammatory imbalance in cerebrospinal fluid
title_full_unstemmed	Zika-exposed microcephalic neonates exhibit higher degree of inflammatory imbalance in cerebrospinal fluid
title_sort	zika-exposed microcephalic neonates exhibit higher degree of inflammatory imbalance in cerebrospinal fluid
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6edef74aae5a4a818c4383510cc58623
work_keys_str_mv	AT gustavocnascimentocarvalho zikaexposedmicrocephalicneonatesexhibithigherdegreeofinflammatoryimbalanceincerebrospinalfluid AT eduardocnascimentocarvalho zikaexposedmicrocephalicneonatesexhibithigherdegreeofinflammatoryimbalanceincerebrospinalfluid AT claralramos zikaexposedmicrocephalicneonatesexhibithigherdegreeofinflammatoryimbalanceincerebrospinalfluid AT analuisavilasboas zikaexposedmicrocephalicneonatesexhibithigherdegreeofinflammatoryimbalanceincerebrospinalfluid AT otavioamorenocarvalho zikaexposedmicrocephalicneonatesexhibithigherdegreeofinflammatoryimbalanceincerebrospinalfluid AT caianlvinhaes zikaexposedmicrocephalicneonatesexhibithigherdegreeofinflammatoryimbalanceincerebrospinalfluid AT beatrizbarretoduarte zikaexposedmicrocephalicneonatesexhibithigherdegreeofinflammatoryimbalanceincerebrospinalfluid AT arturtlqueiroz zikaexposedmicrocephalicneonatesexhibithigherdegreeofinflammatoryimbalanceincerebrospinalfluid AT brunobandrade zikaexposedmicrocephalicneonatesexhibithigherdegreeofinflammatoryimbalanceincerebrospinalfluid AT cristianamnascimentocarvalho zikaexposedmicrocephalicneonatesexhibithigherdegreeofinflammatoryimbalanceincerebrospinalfluid
_version_	1718380126141415424

Zika-exposed microcephalic neonates exhibit higher degree of inflammatory imbalance in cerebrospinal fluid

Ejemplares similares