Circulating Antibodies to Skin Bacteria Detected by Serological Lateral Flow Immunoassays Differentially Correlated With Bacterial Abundance

The serological lateral flow immunoassay (LFIA) was used to detect circulating antibodies to skin bacteria. Next-generation sequencing analysis of the skin microbiome revealed a high relative abundance of Cutibacterium acnes but low abundance of Staphylococcus aureus and Corynebacterium aurimucosum...

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Autores principales:	Ryan Yuki Huang, Chuen Neng Lee, Shabbir Moochhala
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	acne vulgaris antibody C. acnes C. aurimucosum lateral flow immunoassays S. aureus Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/6edef923501d49c3abf096a86af29ab2
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spelling	oai:doaj.org-article:6edef923501d49c3abf096a86af29ab22021-11-10T08:14:12ZCirculating Antibodies to Skin Bacteria Detected by Serological Lateral Flow Immunoassays Differentially Correlated With Bacterial Abundance1664-302X10.3389/fmicb.2021.709562https://doaj.org/article/6edef923501d49c3abf096a86af29ab22021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.709562/fullhttps://doaj.org/toc/1664-302XThe serological lateral flow immunoassay (LFIA) was used to detect circulating antibodies to skin bacteria. Next-generation sequencing analysis of the skin microbiome revealed a high relative abundance of Cutibacterium acnes but low abundance of Staphylococcus aureus and Corynebacterium aurimucosum on human facial samples. Yet, results from both LFIA and antibody titer quantification in 96-well microplates illustrated antibody titers that were not correspondent, and instead negatively correlated, to their respective abundance with human blood containing higher concentrations of antibodies to both S. aureus and C. aurimucosum than C. acnes. Acne vulgaris develops several unique microbial and cellular features, but its correlation with circulating antibodies to bacteria in the pilosebaceous unit remains unknown. Results here revealed that antibodies to C. acnes and S. aureus were approximately 3-fold higher and 1.5-fold lower, respectively, in acne patients than in healthy subjects. Although the results can be further validated by larger sample sizes, the proof-of-concept study demonstrates a newfound discrepancy between the abundance of skin bacteria and amounts of their corresponding antibodies. And in light of acne-correlated amplified titers of specific anticommensal antibodies, we highlight that profiling these antibodies in the pilosebaceous unit by LFIAs may provide a unique signature for monitoring acne vulgaris.Ryan Yuki HuangChuen Neng LeeShabbir MoochhalaFrontiers Media S.A.articleacne vulgarisantibodyC. acnesC. aurimucosumlateral flow immunoassaysS. aureusMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acne vulgaris antibody C. acnes C. aurimucosum lateral flow immunoassays S. aureus Microbiology QR1-502
spellingShingle	acne vulgaris antibody C. acnes C. aurimucosum lateral flow immunoassays S. aureus Microbiology QR1-502 Ryan Yuki Huang Chuen Neng Lee Shabbir Moochhala Circulating Antibodies to Skin Bacteria Detected by Serological Lateral Flow Immunoassays Differentially Correlated With Bacterial Abundance
description	The serological lateral flow immunoassay (LFIA) was used to detect circulating antibodies to skin bacteria. Next-generation sequencing analysis of the skin microbiome revealed a high relative abundance of Cutibacterium acnes but low abundance of Staphylococcus aureus and Corynebacterium aurimucosum on human facial samples. Yet, results from both LFIA and antibody titer quantification in 96-well microplates illustrated antibody titers that were not correspondent, and instead negatively correlated, to their respective abundance with human blood containing higher concentrations of antibodies to both S. aureus and C. aurimucosum than C. acnes. Acne vulgaris develops several unique microbial and cellular features, but its correlation with circulating antibodies to bacteria in the pilosebaceous unit remains unknown. Results here revealed that antibodies to C. acnes and S. aureus were approximately 3-fold higher and 1.5-fold lower, respectively, in acne patients than in healthy subjects. Although the results can be further validated by larger sample sizes, the proof-of-concept study demonstrates a newfound discrepancy between the abundance of skin bacteria and amounts of their corresponding antibodies. And in light of acne-correlated amplified titers of specific anticommensal antibodies, we highlight that profiling these antibodies in the pilosebaceous unit by LFIAs may provide a unique signature for monitoring acne vulgaris.
format	article
author	Ryan Yuki Huang Chuen Neng Lee Shabbir Moochhala
author_facet	Ryan Yuki Huang Chuen Neng Lee Shabbir Moochhala
author_sort	Ryan Yuki Huang
title	Circulating Antibodies to Skin Bacteria Detected by Serological Lateral Flow Immunoassays Differentially Correlated With Bacterial Abundance
title_short	Circulating Antibodies to Skin Bacteria Detected by Serological Lateral Flow Immunoassays Differentially Correlated With Bacterial Abundance
title_full	Circulating Antibodies to Skin Bacteria Detected by Serological Lateral Flow Immunoassays Differentially Correlated With Bacterial Abundance
title_fullStr	Circulating Antibodies to Skin Bacteria Detected by Serological Lateral Flow Immunoassays Differentially Correlated With Bacterial Abundance
title_full_unstemmed	Circulating Antibodies to Skin Bacteria Detected by Serological Lateral Flow Immunoassays Differentially Correlated With Bacterial Abundance
title_sort	circulating antibodies to skin bacteria detected by serological lateral flow immunoassays differentially correlated with bacterial abundance
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6edef923501d49c3abf096a86af29ab2
work_keys_str_mv	AT ryanyukihuang circulatingantibodiestoskinbacteriadetectedbyserologicallateralflowimmunoassaysdifferentiallycorrelatedwithbacterialabundance AT chuennenglee circulatingantibodiestoskinbacteriadetectedbyserologicallateralflowimmunoassaysdifferentiallycorrelatedwithbacterialabundance AT shabbirmoochhala circulatingantibodiestoskinbacteriadetectedbyserologicallateralflowimmunoassaysdifferentiallycorrelatedwithbacterialabundance
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Circulating Antibodies to Skin Bacteria Detected by Serological Lateral Flow Immunoassays Differentially Correlated With Bacterial Abundance

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