A draft genome provides hypotheses on drought tolerance in a keystone plant species in Western North America threatened by climate change
Abstract Climate change presents distinct ecological and physiological challenges to plants as extreme climate events become more common. Understanding how species have adapted to drought, especially ecologically important nonmodel organisms, will be crucial to elucidate potential biological pathway...
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Wiley
2021
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oai:doaj.org-article:6ee0eac331c24693b6eb412b98c484ab2021-11-08T17:10:41ZA draft genome provides hypotheses on drought tolerance in a keystone plant species in Western North America threatened by climate change2045-775810.1002/ece3.8245https://doaj.org/article/6ee0eac331c24693b6eb412b98c484ab2021-11-01T00:00:00Zhttps://doi.org/10.1002/ece3.8245https://doaj.org/toc/2045-7758Abstract Climate change presents distinct ecological and physiological challenges to plants as extreme climate events become more common. Understanding how species have adapted to drought, especially ecologically important nonmodel organisms, will be crucial to elucidate potential biological pathways for drought adaptation and inform conservation strategies. To aid in genome‐to‐phenome research, a draft genome was assembled for a diploid individual of Artemisia tridentata subsp. tridentata, a threatened keystone shrub in western North America. While this taxon has few genetic resources available and genetic/genomics work has proven difficult due to genetic heterozygosity in the past, a draft genome was successfully assembled. Aquaporin (AQP) genes and their promoter sequences were mined from the draft genome to predict mechanisms regulating gene expression and generate hypotheses on key genes underpinning drought response. Fifty‐one AQP genes were fully assembled within the draft genome. Promoter and phylogenetic analyses revealed putative duplicates of A. tridentata subsp. tridentata AQPs which have experienced differentiation in promoter elements, potentially supporting novel biological pathways. Comparison with nondrought‐tolerant congener supports enrichments of AQP genes in this taxon during adaptation to drought stress. Differentiation of promoter elements revealed that paralogues of some genes have evolved to function in different pathways, highlighting these genes as potential candidates for future research and providing critical hypotheses for future genome‐to‐phenome work.Anthony E. MeltonJames BeckStephanie J. GallaJerry JenkinsLori HandleyMin KimJane GrimwoodJeremy SchmutzBryce A. RichardsonMarcelo SerpeStephen NovakSven BuerkiWileyarticleadaptationaquaporinsdrought stressgenome mininggenome‐to‐phenomesagebrushEcologyQH540-549.5ENEcology and Evolution, Vol 11, Iss 21, Pp 15417-15429 (2021) |
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DOAJ |
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adaptation aquaporins drought stress genome mining genome‐to‐phenome sagebrush Ecology QH540-549.5 |
| spellingShingle |
adaptation aquaporins drought stress genome mining genome‐to‐phenome sagebrush Ecology QH540-549.5 Anthony E. Melton James Beck Stephanie J. Galla Jerry Jenkins Lori Handley Min Kim Jane Grimwood Jeremy Schmutz Bryce A. Richardson Marcelo Serpe Stephen Novak Sven Buerki A draft genome provides hypotheses on drought tolerance in a keystone plant species in Western North America threatened by climate change |
| description |
Abstract Climate change presents distinct ecological and physiological challenges to plants as extreme climate events become more common. Understanding how species have adapted to drought, especially ecologically important nonmodel organisms, will be crucial to elucidate potential biological pathways for drought adaptation and inform conservation strategies. To aid in genome‐to‐phenome research, a draft genome was assembled for a diploid individual of Artemisia tridentata subsp. tridentata, a threatened keystone shrub in western North America. While this taxon has few genetic resources available and genetic/genomics work has proven difficult due to genetic heterozygosity in the past, a draft genome was successfully assembled. Aquaporin (AQP) genes and their promoter sequences were mined from the draft genome to predict mechanisms regulating gene expression and generate hypotheses on key genes underpinning drought response. Fifty‐one AQP genes were fully assembled within the draft genome. Promoter and phylogenetic analyses revealed putative duplicates of A. tridentata subsp. tridentata AQPs which have experienced differentiation in promoter elements, potentially supporting novel biological pathways. Comparison with nondrought‐tolerant congener supports enrichments of AQP genes in this taxon during adaptation to drought stress. Differentiation of promoter elements revealed that paralogues of some genes have evolved to function in different pathways, highlighting these genes as potential candidates for future research and providing critical hypotheses for future genome‐to‐phenome work. |
| format |
article |
| author |
Anthony E. Melton James Beck Stephanie J. Galla Jerry Jenkins Lori Handley Min Kim Jane Grimwood Jeremy Schmutz Bryce A. Richardson Marcelo Serpe Stephen Novak Sven Buerki |
| author_facet |
Anthony E. Melton James Beck Stephanie J. Galla Jerry Jenkins Lori Handley Min Kim Jane Grimwood Jeremy Schmutz Bryce A. Richardson Marcelo Serpe Stephen Novak Sven Buerki |
| author_sort |
Anthony E. Melton |
| title |
A draft genome provides hypotheses on drought tolerance in a keystone plant species in Western North America threatened by climate change |
| title_short |
A draft genome provides hypotheses on drought tolerance in a keystone plant species in Western North America threatened by climate change |
| title_full |
A draft genome provides hypotheses on drought tolerance in a keystone plant species in Western North America threatened by climate change |
| title_fullStr |
A draft genome provides hypotheses on drought tolerance in a keystone plant species in Western North America threatened by climate change |
| title_full_unstemmed |
A draft genome provides hypotheses on drought tolerance in a keystone plant species in Western North America threatened by climate change |
| title_sort |
draft genome provides hypotheses on drought tolerance in a keystone plant species in western north america threatened by climate change |
| publisher |
Wiley |
| publishDate |
2021 |
| url |
https://doaj.org/article/6ee0eac331c24693b6eb412b98c484ab |
| work_keys_str_mv |
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1718441483710758912 |