High-Resolution Patterned Cellular Constructs by Droplet-Based 3D Printing

Abstract Bioprinting is an emerging technique for the fabrication of living tissues that allows cells to be arranged in predetermined three-dimensional (3D) architectures. However, to date, there are limited examples of bioprinted constructs containing multiple cell types patterned at high-resolutio...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alexander D. Graham, Sam N. Olof, Madeline J. Burke, James P. K. Armstrong, Ellina A. Mikhailova, James G. Nicholson, Stuart J. Box, Francis G. Szele, Adam W. Perriman, Hagan Bayley
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ef32776ef0345cfae25068ba97a4797
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6ef32776ef0345cfae25068ba97a4797
record_format	dspace
spelling	oai:doaj.org-article:6ef32776ef0345cfae25068ba97a47972021-12-02T16:06:32ZHigh-Resolution Patterned Cellular Constructs by Droplet-Based 3D Printing10.1038/s41598-017-06358-x2045-2322https://doaj.org/article/6ef32776ef0345cfae25068ba97a47972017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06358-xhttps://doaj.org/toc/2045-2322Abstract Bioprinting is an emerging technique for the fabrication of living tissues that allows cells to be arranged in predetermined three-dimensional (3D) architectures. However, to date, there are limited examples of bioprinted constructs containing multiple cell types patterned at high-resolution. Here we present a low-cost process that employs 3D printing of aqueous droplets containing mammalian cells to produce robust, patterned constructs in oil, which were reproducibly transferred to culture medium. Human embryonic kidney (HEK) cells and ovine mesenchymal stem cells (oMSCs) were printed at tissue-relevant densities (107 cells mL−1) and a high droplet resolution of 1 nL. High-resolution 3D geometries were printed with features of ≤200 μm; these included an arborised cell junction, a diagonal-plane junction and an osteochondral interface. The printed cells showed high viability (90% on average) and HEK cells within the printed structures were shown to proliferate under culture conditions. Significantly, a five-week tissue engineering study demonstrated that printed oMSCs could be differentiated down the chondrogenic lineage to generate cartilage-like structures containing type II collagen.Alexander D. GrahamSam N. OlofMadeline J. BurkeJames P. K. ArmstrongEllina A. MikhailovaJames G. NicholsonStuart J. BoxFrancis G. SzeleAdam W. PerrimanHagan BayleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexander D. Graham Sam N. Olof Madeline J. Burke James P. K. Armstrong Ellina A. Mikhailova James G. Nicholson Stuart J. Box Francis G. Szele Adam W. Perriman Hagan Bayley High-Resolution Patterned Cellular Constructs by Droplet-Based 3D Printing
description	Abstract Bioprinting is an emerging technique for the fabrication of living tissues that allows cells to be arranged in predetermined three-dimensional (3D) architectures. However, to date, there are limited examples of bioprinted constructs containing multiple cell types patterned at high-resolution. Here we present a low-cost process that employs 3D printing of aqueous droplets containing mammalian cells to produce robust, patterned constructs in oil, which were reproducibly transferred to culture medium. Human embryonic kidney (HEK) cells and ovine mesenchymal stem cells (oMSCs) were printed at tissue-relevant densities (107 cells mL−1) and a high droplet resolution of 1 nL. High-resolution 3D geometries were printed with features of ≤200 μm; these included an arborised cell junction, a diagonal-plane junction and an osteochondral interface. The printed cells showed high viability (90% on average) and HEK cells within the printed structures were shown to proliferate under culture conditions. Significantly, a five-week tissue engineering study demonstrated that printed oMSCs could be differentiated down the chondrogenic lineage to generate cartilage-like structures containing type II collagen.
format	article
author	Alexander D. Graham Sam N. Olof Madeline J. Burke James P. K. Armstrong Ellina A. Mikhailova James G. Nicholson Stuart J. Box Francis G. Szele Adam W. Perriman Hagan Bayley
author_facet	Alexander D. Graham Sam N. Olof Madeline J. Burke James P. K. Armstrong Ellina A. Mikhailova James G. Nicholson Stuart J. Box Francis G. Szele Adam W. Perriman Hagan Bayley
author_sort	Alexander D. Graham
title	High-Resolution Patterned Cellular Constructs by Droplet-Based 3D Printing
title_short	High-Resolution Patterned Cellular Constructs by Droplet-Based 3D Printing
title_full	High-Resolution Patterned Cellular Constructs by Droplet-Based 3D Printing
title_fullStr	High-Resolution Patterned Cellular Constructs by Droplet-Based 3D Printing
title_full_unstemmed	High-Resolution Patterned Cellular Constructs by Droplet-Based 3D Printing
title_sort	high-resolution patterned cellular constructs by droplet-based 3d printing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6ef32776ef0345cfae25068ba97a4797
work_keys_str_mv	AT alexanderdgraham highresolutionpatternedcellularconstructsbydropletbased3dprinting AT samnolof highresolutionpatternedcellularconstructsbydropletbased3dprinting AT madelinejburke highresolutionpatternedcellularconstructsbydropletbased3dprinting AT jamespkarmstrong highresolutionpatternedcellularconstructsbydropletbased3dprinting AT ellinaamikhailova highresolutionpatternedcellularconstructsbydropletbased3dprinting AT jamesgnicholson highresolutionpatternedcellularconstructsbydropletbased3dprinting AT stuartjbox highresolutionpatternedcellularconstructsbydropletbased3dprinting AT francisgszele highresolutionpatternedcellularconstructsbydropletbased3dprinting AT adamwperriman highresolutionpatternedcellularconstructsbydropletbased3dprinting AT haganbayley highresolutionpatternedcellularconstructsbydropletbased3dprinting
_version_	1718384967157809152

High-Resolution Patterned Cellular Constructs by Droplet-Based 3D Printing

Ejemplares similares