Rapid dopaminergic modulation of the fish hypothalamic transcriptome and proteome.

<h4>Background</h4>Dopamine (DA) is a major neurotransmitter playing an important role in the regulation of vertebrate reproduction. We developed a novel method for the comparison of transcriptomic and proteomic data obtained from in vivo experiments designed to study the neuroendocrine...

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Autores principales:	Jason T Popesku, Christopher J Martyniuk, Nancy D Denslow, Vance L Trudeau
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ef49a9389c747eb91acb50cde979afe
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spelling	oai:doaj.org-article:6ef49a9389c747eb91acb50cde979afe2021-11-18T06:35:51ZRapid dopaminergic modulation of the fish hypothalamic transcriptome and proteome.1932-620310.1371/journal.pone.0012338https://doaj.org/article/6ef49a9389c747eb91acb50cde979afe2010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20808832/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Dopamine (DA) is a major neurotransmitter playing an important role in the regulation of vertebrate reproduction. We developed a novel method for the comparison of transcriptomic and proteomic data obtained from in vivo experiments designed to study the neuroendocrine actions of DA.<h4>Methods and findings</h4>Female goldfish were injected (i.p.) with DA agonists (D1-specific; SKF 38393, or D2-specific; LY 171555) and sacrificed after 5 h. Serum LH levels were reduced by 57% and 75% by SKF 38393 and LY 171555, respectively, indicating that the treatments produced physiologically relevant responses in vivo. Bioinformatic strategies and a ray-finned fish database were established for microarray and iTRAQ proteomic analysis of the hypothalamus, revealing a total of 3088 mRNAs and 42 proteins as being differentially regulated by the treatments. Twenty one proteins and mRNAs corresponding to these proteins appeared on both lists. Many of the mRNAs and proteins affected by the treatments were grouped into the Gene Ontology categorizations of protein complex, signal transduction, response to stimulus, and regulation of cellular processes. There was a 57% and 14% directional agreement between the differentially-regulated mRNAs and proteins for SKF 38393 and LY 171555, respectively.<h4>Conclusions</h4>The results demonstrate the applicability of advanced high-throughput genomic and proteomic analyses in an amendable well-studied teleost model species whose genome has yet to be sequenced. We demonstrate that DA rapidly regulates multiple hypothalamic pathways and processes that are also known to be involved in pathologies of the central nervous system.Jason T PopeskuChristopher J MartyniukNancy D DenslowVance L TrudeauPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 8, p e12338 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jason T Popesku Christopher J Martyniuk Nancy D Denslow Vance L Trudeau Rapid dopaminergic modulation of the fish hypothalamic transcriptome and proteome.
description	<h4>Background</h4>Dopamine (DA) is a major neurotransmitter playing an important role in the regulation of vertebrate reproduction. We developed a novel method for the comparison of transcriptomic and proteomic data obtained from in vivo experiments designed to study the neuroendocrine actions of DA.<h4>Methods and findings</h4>Female goldfish were injected (i.p.) with DA agonists (D1-specific; SKF 38393, or D2-specific; LY 171555) and sacrificed after 5 h. Serum LH levels were reduced by 57% and 75% by SKF 38393 and LY 171555, respectively, indicating that the treatments produced physiologically relevant responses in vivo. Bioinformatic strategies and a ray-finned fish database were established for microarray and iTRAQ proteomic analysis of the hypothalamus, revealing a total of 3088 mRNAs and 42 proteins as being differentially regulated by the treatments. Twenty one proteins and mRNAs corresponding to these proteins appeared on both lists. Many of the mRNAs and proteins affected by the treatments were grouped into the Gene Ontology categorizations of protein complex, signal transduction, response to stimulus, and regulation of cellular processes. There was a 57% and 14% directional agreement between the differentially-regulated mRNAs and proteins for SKF 38393 and LY 171555, respectively.<h4>Conclusions</h4>The results demonstrate the applicability of advanced high-throughput genomic and proteomic analyses in an amendable well-studied teleost model species whose genome has yet to be sequenced. We demonstrate that DA rapidly regulates multiple hypothalamic pathways and processes that are also known to be involved in pathologies of the central nervous system.
format	article
author	Jason T Popesku Christopher J Martyniuk Nancy D Denslow Vance L Trudeau
author_facet	Jason T Popesku Christopher J Martyniuk Nancy D Denslow Vance L Trudeau
author_sort	Jason T Popesku
title	Rapid dopaminergic modulation of the fish hypothalamic transcriptome and proteome.
title_short	Rapid dopaminergic modulation of the fish hypothalamic transcriptome and proteome.
title_full	Rapid dopaminergic modulation of the fish hypothalamic transcriptome and proteome.
title_fullStr	Rapid dopaminergic modulation of the fish hypothalamic transcriptome and proteome.
title_full_unstemmed	Rapid dopaminergic modulation of the fish hypothalamic transcriptome and proteome.
title_sort	rapid dopaminergic modulation of the fish hypothalamic transcriptome and proteome.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/6ef49a9389c747eb91acb50cde979afe
work_keys_str_mv	AT jasontpopesku rapiddopaminergicmodulationofthefishhypothalamictranscriptomeandproteome AT christopherjmartyniuk rapiddopaminergicmodulationofthefishhypothalamictranscriptomeandproteome AT nancyddenslow rapiddopaminergicmodulationofthefishhypothalamictranscriptomeandproteome AT vanceltrudeau rapiddopaminergicmodulationofthefishhypothalamictranscriptomeandproteome
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Rapid dopaminergic modulation of the fish hypothalamic transcriptome and proteome.

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