Β-Sitosterol of Red Dragon Fruit (Hylocereus Polyrhizus) and Its Response to Macrophage And Nitric Oxide

Hylocerius polyrhizus has relatively big potency as natural antioxidant. The compound considered as antioxidant also has immunomodulatory activity. This study showed isolation for identifying the active compounds in H. polyrhizus peels that are able to increase immune system of human body. The metha...

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Autores principales:	Sri wahdaningsih, Subagus Wahyuono, Sugeng Riyanto, Retno Murwanti
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	h. polyrhizus, β-sitosterol, macrophage, nitrit oxide Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/6f0007b4d98c4b648b811c9343a93e9f
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spelling	oai:doaj.org-article:6f0007b4d98c4b648b811c9343a93e9f2021-11-15T04:09:03ZΒ-Sitosterol of Red Dragon Fruit (Hylocereus Polyrhizus) and Its Response to Macrophage And Nitric Oxide2338-94272338-948610.22146/ijp.2331https://doaj.org/article/6f0007b4d98c4b648b811c9343a93e9f2021-09-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/2331https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Hylocerius polyrhizus has relatively big potency as natural antioxidant. The compound considered as antioxidant also has immunomodulatory activity. This study showed isolation for identifying the active compounds in H. polyrhizus peels that are able to increase immune system of human body. The methanol extracts were partitioned and fractionated. The active compounds of petroleum ether fraction were partitioned and purified using Preparative Thin Layer Chromatography (PTLC). The identification of active compound structures was done with spectroscopy: UV, FT-IR, 13CNMR, 1HNMR, DEPT and HSQC. The immunomodulatory activity was also tested. Based on the spectroscopic data, the identified isolate was β-sitosterol. The statistical analysis of macrophage cell activity and nitric oxide showed that isolates at the highest concentrations of 100 µg/mL were able to activate macrophage cells and enhance the production of nitric oxide.Sri wahdaningsihSubagus WahyuonoSugeng RiyantoRetno MurwantiUniversitas Gadjah Madaarticleh. polyrhizus, β-sitosterol, macrophage, nitrit oxidePharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 399-407 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	h. polyrhizus, β-sitosterol, macrophage, nitrit oxide Pharmacy and materia medica RS1-441
spellingShingle	h. polyrhizus, β-sitosterol, macrophage, nitrit oxide Pharmacy and materia medica RS1-441 Sri wahdaningsih Subagus Wahyuono Sugeng Riyanto Retno Murwanti Β-Sitosterol of Red Dragon Fruit (Hylocereus Polyrhizus) and Its Response to Macrophage And Nitric Oxide
description	Hylocerius polyrhizus has relatively big potency as natural antioxidant. The compound considered as antioxidant also has immunomodulatory activity. This study showed isolation for identifying the active compounds in H. polyrhizus peels that are able to increase immune system of human body. The methanol extracts were partitioned and fractionated. The active compounds of petroleum ether fraction were partitioned and purified using Preparative Thin Layer Chromatography (PTLC). The identification of active compound structures was done with spectroscopy: UV, FT-IR, 13CNMR, 1HNMR, DEPT and HSQC. The immunomodulatory activity was also tested. Based on the spectroscopic data, the identified isolate was β-sitosterol. The statistical analysis of macrophage cell activity and nitric oxide showed that isolates at the highest concentrations of 100 µg/mL were able to activate macrophage cells and enhance the production of nitric oxide.
format	article
author	Sri wahdaningsih Subagus Wahyuono Sugeng Riyanto Retno Murwanti
author_facet	Sri wahdaningsih Subagus Wahyuono Sugeng Riyanto Retno Murwanti
author_sort	Sri wahdaningsih
title	Β-Sitosterol of Red Dragon Fruit (Hylocereus Polyrhizus) and Its Response to Macrophage And Nitric Oxide
title_short	Β-Sitosterol of Red Dragon Fruit (Hylocereus Polyrhizus) and Its Response to Macrophage And Nitric Oxide
title_full	Β-Sitosterol of Red Dragon Fruit (Hylocereus Polyrhizus) and Its Response to Macrophage And Nitric Oxide
title_fullStr	Β-Sitosterol of Red Dragon Fruit (Hylocereus Polyrhizus) and Its Response to Macrophage And Nitric Oxide
title_full_unstemmed	Β-Sitosterol of Red Dragon Fruit (Hylocereus Polyrhizus) and Its Response to Macrophage And Nitric Oxide
title_sort	β-sitosterol of red dragon fruit (hylocereus polyrhizus) and its response to macrophage and nitric oxide
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/6f0007b4d98c4b648b811c9343a93e9f
work_keys_str_mv	AT sriwahdaningsih bsitosterolofreddragonfruithylocereuspolyrhizusanditsresponsetomacrophageandnitricoxide AT subaguswahyuono bsitosterolofreddragonfruithylocereuspolyrhizusanditsresponsetomacrophageandnitricoxide AT sugengriyanto bsitosterolofreddragonfruithylocereuspolyrhizusanditsresponsetomacrophageandnitricoxide AT retnomurwanti bsitosterolofreddragonfruithylocereuspolyrhizusanditsresponsetomacrophageandnitricoxide
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Β-Sitosterol of Red Dragon Fruit (Hylocereus Polyrhizus) and Its Response to Macrophage And Nitric Oxide

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