Distribution and characterization of progenitor cells within the human filum terminale.

<h4>Background</h4>Filum terminale (FT) is a structure that is intimately associated with conus medullaris, the most caudal part of the spinal cord. It is well documented that certain regions of the adult human central nervous system contains undifferentiated, progenitor cells or multipo...

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Autores principales:	Lisa Arvidsson, Michael Fagerlund, Nasren Jaff, Amina Ossoinak, Katarina Jansson, Anders Hägerstrand, Clas B Johansson, Lou Brundin, Mikael Svensson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6f05ee60afbb49cf9fc6d3859011a654
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spelling	oai:doaj.org-article:6f05ee60afbb49cf9fc6d3859011a6542021-11-18T07:34:29ZDistribution and characterization of progenitor cells within the human filum terminale.1932-620310.1371/journal.pone.0027393https://doaj.org/article/6f05ee60afbb49cf9fc6d3859011a6542011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22096566/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Filum terminale (FT) is a structure that is intimately associated with conus medullaris, the most caudal part of the spinal cord. It is well documented that certain regions of the adult human central nervous system contains undifferentiated, progenitor cells or multipotent precursors. The primary objective of this study was to describe the distribution and progenitor features of this cell population in humans, and to confirm their ability to differentiate within the neuroectodermal lineage.<h4>Methodology/principal findings</h4>We demonstrate that neural stem/progenitor cells are present in FT obtained from patients treated for tethered cord. When human or rat FT-derived cells were cultured in defined medium, they proliferated and formed neurospheres in 13 out of 21 individuals. Cells expressing Sox2 and Musashi-1 were found to outline the central canal, and also to be distributed in islets throughout the whole FT. Following plating, the cells developed antigen profiles characteristic of astrocytes (GFAP) and neurons (β-III-tubulin). Addition of PDGF-BB directed the cells towards a neuronal fate. Moreover, the cells obtained from young donors shows higher capacity for proliferation and are easier to expand than cells derived from older donors.<h4>Conclusion/significance</h4>The identification of bona fide neural progenitor cells in FT suggests a possible role for progenitor cells in this extension of conus medullaris and may provide an additional source of such cells for possible therapeutic purposes. Filum terminale, human, progenitor cells, neuron, astrocytes, spinal cord.Lisa ArvidssonMichael FagerlundNasren JaffAmina OssoinakKatarina JanssonAnders HägerstrandClas B JohanssonLou BrundinMikael SvenssonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27393 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lisa Arvidsson Michael Fagerlund Nasren Jaff Amina Ossoinak Katarina Jansson Anders Hägerstrand Clas B Johansson Lou Brundin Mikael Svensson Distribution and characterization of progenitor cells within the human filum terminale.
description	<h4>Background</h4>Filum terminale (FT) is a structure that is intimately associated with conus medullaris, the most caudal part of the spinal cord. It is well documented that certain regions of the adult human central nervous system contains undifferentiated, progenitor cells or multipotent precursors. The primary objective of this study was to describe the distribution and progenitor features of this cell population in humans, and to confirm their ability to differentiate within the neuroectodermal lineage.<h4>Methodology/principal findings</h4>We demonstrate that neural stem/progenitor cells are present in FT obtained from patients treated for tethered cord. When human or rat FT-derived cells were cultured in defined medium, they proliferated and formed neurospheres in 13 out of 21 individuals. Cells expressing Sox2 and Musashi-1 were found to outline the central canal, and also to be distributed in islets throughout the whole FT. Following plating, the cells developed antigen profiles characteristic of astrocytes (GFAP) and neurons (β-III-tubulin). Addition of PDGF-BB directed the cells towards a neuronal fate. Moreover, the cells obtained from young donors shows higher capacity for proliferation and are easier to expand than cells derived from older donors.<h4>Conclusion/significance</h4>The identification of bona fide neural progenitor cells in FT suggests a possible role for progenitor cells in this extension of conus medullaris and may provide an additional source of such cells for possible therapeutic purposes. Filum terminale, human, progenitor cells, neuron, astrocytes, spinal cord.
format	article
author	Lisa Arvidsson Michael Fagerlund Nasren Jaff Amina Ossoinak Katarina Jansson Anders Hägerstrand Clas B Johansson Lou Brundin Mikael Svensson
author_facet	Lisa Arvidsson Michael Fagerlund Nasren Jaff Amina Ossoinak Katarina Jansson Anders Hägerstrand Clas B Johansson Lou Brundin Mikael Svensson
author_sort	Lisa Arvidsson
title	Distribution and characterization of progenitor cells within the human filum terminale.
title_short	Distribution and characterization of progenitor cells within the human filum terminale.
title_full	Distribution and characterization of progenitor cells within the human filum terminale.
title_fullStr	Distribution and characterization of progenitor cells within the human filum terminale.
title_full_unstemmed	Distribution and characterization of progenitor cells within the human filum terminale.
title_sort	distribution and characterization of progenitor cells within the human filum terminale.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/6f05ee60afbb49cf9fc6d3859011a654
work_keys_str_mv	AT lisaarvidsson distributionandcharacterizationofprogenitorcellswithinthehumanfilumterminale AT michaelfagerlund distributionandcharacterizationofprogenitorcellswithinthehumanfilumterminale AT nasrenjaff distributionandcharacterizationofprogenitorcellswithinthehumanfilumterminale AT aminaossoinak distributionandcharacterizationofprogenitorcellswithinthehumanfilumterminale AT katarinajansson distributionandcharacterizationofprogenitorcellswithinthehumanfilumterminale AT andershagerstrand distributionandcharacterizationofprogenitorcellswithinthehumanfilumterminale AT clasbjohansson distributionandcharacterizationofprogenitorcellswithinthehumanfilumterminale AT loubrundin distributionandcharacterizationofprogenitorcellswithinthehumanfilumterminale AT mikaelsvensson distributionandcharacterizationofprogenitorcellswithinthehumanfilumterminale
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Distribution and characterization of progenitor cells within the human filum terminale.

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