Selective Separation and Analysis of Catecholamines in Urine Based on Magnetic Solid Phase Extraction by Mercaptophenylboronic Acid Functionalized Fe3O4-NH2@Au Magnetic Nanoparticles Coupled with HPLC

A novel magnetic solid phase extraction based on mercaptophenylboronic acid (MPBA)-functionalized Fe3O4-NH2@Au nanomaterial (Fe3O4-NH2@Au-MPBA) was developed for...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Qing Han, Xiaoxiao Wu, Yi Cao, Hua Zhang, Yuqin Zhao, Xuejun Kang, Huaiyuan Zhu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au magnetic solid phase extraction catecholamines HPLC electrochemical detection urine Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/6f11767389754118b1e48a92a9847263
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6f11767389754118b1e48a92a9847263
record_format	dspace
spelling	oai:doaj.org-article:6f11767389754118b1e48a92a98472632021-11-25T18:59:16ZSelective Separation and Analysis of Catecholamines in Urine Based on Magnetic Solid Phase Extraction by Mercaptophenylboronic Acid Functionalized Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au Magnetic Nanoparticles Coupled with HPLC10.3390/separations81101962297-8739https://doaj.org/article/6f11767389754118b1e48a92a98472632021-10-01T00:00:00Zhttps://www.mdpi.com/2297-8739/8/11/196https://doaj.org/toc/2297-8739A novel magnetic solid phase extraction based on mercaptophenylboronic acid (MPBA)-functionalized Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au nanomaterial (Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au-MPBA) was developed for selective separation and enrichment of catecholamines (including dopamine, norepinephrine and adrenaline). Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au-MPBA nanoparticles were achieved by self-assembly-anchoring MPBA molecules on the surface of Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au nanocomposites, which were synthesized via a facial ultrasonic auxiliary in situ reduction process. The interaction between cis-diol from catecholamines and boronic acid was reversible and could be flexibly controlled by adjusting pH value. The catecholamines could be quickly adsorbed by Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au-MPBA in weak alkaline solution (pH 8.0–9.0) and subsequently released in acid solution (pH 1.0–2.0). The process of adsorption and dissociation was very fast. Furthermore, the three catecholamines could be detected in urine from children by high performance liquid chromatography (HPLC) with electrochemical detector. Under optimal conditions, norepinephrine (NE), epinephrine (EP) and dopamine (DA) were separated very well from internal standard and exhibited a good linearity in the range of 2.5–500.0 ng mL<sup>−1</sup>, with correlation coefficients of r<sup>2</sup> > 0.9907. Limits of detection (LOD) (signal to noise = 3) were 0.39, 0.27 and 0.60 ng mL<sup>−1</sup> for NE, EP and DA, respectively. Recoveries for the spiked catecholamines were in the range of 85.4–105.2% with the relative standard deviation (RSD) < 11.5%.Qing HanXiaoxiao WuYi CaoHua ZhangYuqin ZhaoXuejun KangHuaiyuan ZhuMDPI AGarticleFe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Aumagnetic solid phase extractioncatecholaminesHPLCelectrochemical detectionurinePhysicsQC1-999ChemistryQD1-999ENSeparations, Vol 8, Iss 196, p 196 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au magnetic solid phase extraction catecholamines HPLC electrochemical detection urine Physics QC1-999 Chemistry QD1-999
spellingShingle	Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au magnetic solid phase extraction catecholamines HPLC electrochemical detection urine Physics QC1-999 Chemistry QD1-999 Qing Han Xiaoxiao Wu Yi Cao Hua Zhang Yuqin Zhao Xuejun Kang Huaiyuan Zhu Selective Separation and Analysis of Catecholamines in Urine Based on Magnetic Solid Phase Extraction by Mercaptophenylboronic Acid Functionalized Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au Magnetic Nanoparticles Coupled with HPLC
description	A novel magnetic solid phase extraction based on mercaptophenylboronic acid (MPBA)-functionalized Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au nanomaterial (Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au-MPBA) was developed for selective separation and enrichment of catecholamines (including dopamine, norepinephrine and adrenaline). Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au-MPBA nanoparticles were achieved by self-assembly-anchoring MPBA molecules on the surface of Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au nanocomposites, which were synthesized via a facial ultrasonic auxiliary in situ reduction process. The interaction between cis-diol from catecholamines and boronic acid was reversible and could be flexibly controlled by adjusting pH value. The catecholamines could be quickly adsorbed by Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au-MPBA in weak alkaline solution (pH 8.0–9.0) and subsequently released in acid solution (pH 1.0–2.0). The process of adsorption and dissociation was very fast. Furthermore, the three catecholamines could be detected in urine from children by high performance liquid chromatography (HPLC) with electrochemical detector. Under optimal conditions, norepinephrine (NE), epinephrine (EP) and dopamine (DA) were separated very well from internal standard and exhibited a good linearity in the range of 2.5–500.0 ng mL<sup>−1</sup>, with correlation coefficients of r<sup>2</sup> > 0.9907. Limits of detection (LOD) (signal to noise = 3) were 0.39, 0.27 and 0.60 ng mL<sup>−1</sup> for NE, EP and DA, respectively. Recoveries for the spiked catecholamines were in the range of 85.4–105.2% with the relative standard deviation (RSD) < 11.5%.
format	article
author	Qing Han Xiaoxiao Wu Yi Cao Hua Zhang Yuqin Zhao Xuejun Kang Huaiyuan Zhu
author_facet	Qing Han Xiaoxiao Wu Yi Cao Hua Zhang Yuqin Zhao Xuejun Kang Huaiyuan Zhu
author_sort	Qing Han
title	Selective Separation and Analysis of Catecholamines in Urine Based on Magnetic Solid Phase Extraction by Mercaptophenylboronic Acid Functionalized Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au Magnetic Nanoparticles Coupled with HPLC
title_short	Selective Separation and Analysis of Catecholamines in Urine Based on Magnetic Solid Phase Extraction by Mercaptophenylboronic Acid Functionalized Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au Magnetic Nanoparticles Coupled with HPLC
title_full	Selective Separation and Analysis of Catecholamines in Urine Based on Magnetic Solid Phase Extraction by Mercaptophenylboronic Acid Functionalized Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au Magnetic Nanoparticles Coupled with HPLC
title_fullStr	Selective Separation and Analysis of Catecholamines in Urine Based on Magnetic Solid Phase Extraction by Mercaptophenylboronic Acid Functionalized Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au Magnetic Nanoparticles Coupled with HPLC
title_full_unstemmed	Selective Separation and Analysis of Catecholamines in Urine Based on Magnetic Solid Phase Extraction by Mercaptophenylboronic Acid Functionalized Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au Magnetic Nanoparticles Coupled with HPLC
title_sort	selective separation and analysis of catecholamines in urine based on magnetic solid phase extraction by mercaptophenylboronic acid functionalized fe<sub>3</sub>o<sub>4</sub>-nh<sub>2</sub>@au magnetic nanoparticles coupled with hplc
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6f11767389754118b1e48a92a9847263
work_keys_str_mv	AT qinghan selectiveseparationandanalysisofcatecholaminesinurinebasedonmagneticsolidphaseextractionbymercaptophenylboronicacidfunctionalizedfesub3subosub4subnhsub2subaumagneticnanoparticlescoupledwithhplc AT xiaoxiaowu selectiveseparationandanalysisofcatecholaminesinurinebasedonmagneticsolidphaseextractionbymercaptophenylboronicacidfunctionalizedfesub3subosub4subnhsub2subaumagneticnanoparticlescoupledwithhplc AT yicao selectiveseparationandanalysisofcatecholaminesinurinebasedonmagneticsolidphaseextractionbymercaptophenylboronicacidfunctionalizedfesub3subosub4subnhsub2subaumagneticnanoparticlescoupledwithhplc AT huazhang selectiveseparationandanalysisofcatecholaminesinurinebasedonmagneticsolidphaseextractionbymercaptophenylboronicacidfunctionalizedfesub3subosub4subnhsub2subaumagneticnanoparticlescoupledwithhplc AT yuqinzhao selectiveseparationandanalysisofcatecholaminesinurinebasedonmagneticsolidphaseextractionbymercaptophenylboronicacidfunctionalizedfesub3subosub4subnhsub2subaumagneticnanoparticlescoupledwithhplc AT xuejunkang selectiveseparationandanalysisofcatecholaminesinurinebasedonmagneticsolidphaseextractionbymercaptophenylboronicacidfunctionalizedfesub3subosub4subnhsub2subaumagneticnanoparticlescoupledwithhplc AT huaiyuanzhu selectiveseparationandanalysisofcatecholaminesinurinebasedonmagneticsolidphaseextractionbymercaptophenylboronicacidfunctionalizedfesub3subosub4subnhsub2subaumagneticnanoparticlescoupledwithhplc
_version_	1718410467448193024

Selective Separation and Analysis of Catecholamines in Urine Based on Magnetic Solid Phase Extraction by Mercaptophenylboronic Acid Functionalized Fe<sub>3</sub>O<sub>4</sub>-NH<sub>2</sub>@Au Magnetic Nanoparticles Coupled with HPLC

Ejemplares similares