Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles

Kai-Wen Huang1, Shieh-Yueh Yang2,3, Yu-Wei Hong3, Jen-Jie Chieh3, Che-Chuan Yang3, Herng-Er Horng3, Chau-Chung Wu4, Chin-Yih Hong5, Hong-Chang Yang61Department of Surgery and Hepatitis Research Center, National Taiwan University Hospital, National Taiwan University, Taipei, 2MagQu Co, Ltd, Sindian D...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Huang KW, Yang SY, Hong YW, Chieh JJ, Yang CC, Horng HE, Wu CC, Hong CY, Yang HC
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2012
Materias:
Acceso en línea:https://doaj.org/article/6f132fbf43be43ae9cf76f958278defa
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:6f132fbf43be43ae9cf76f958278defa
record_format dspace
spelling oai:doaj.org-article:6f132fbf43be43ae9cf76f958278defa2021-12-02T02:42:15ZFeasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles1176-91141178-2013https://doaj.org/article/6f132fbf43be43ae9cf76f958278defa2012-04-01T00:00:00Zhttp://www.dovepress.com/feasibility-studies-for-assaying-alpha-fetoprotein-using-antibody-acti-a9710https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Kai-Wen Huang1, Shieh-Yueh Yang2,3, Yu-Wei Hong3, Jen-Jie Chieh3, Che-Chuan Yang3, Herng-Er Horng3, Chau-Chung Wu4, Chin-Yih Hong5, Hong-Chang Yang61Department of Surgery and Hepatitis Research Center, National Taiwan University Hospital, National Taiwan University, Taipei, 2MagQu Co, Ltd, Sindian Dist, New Taipei City, 3Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei, 4Departments of Internal Medicine and Primary Care Medicine, College of Medicine, National Taiwan University, Taipei, 5Graduate Institute of Bio-medical Engineering, National Chung Hsing University, Taichung, 6Department of Physics, National Taiwan University, Taipei, TaiwanAbstract: Some previous reports have already shown the characterizations of immunomagnetic reduction (IMR). The assay technology involves the utilities of biofunctionalized magnetic nanoparticles to label target biomolecules. However, the detection threshold and interference tests for IMR have not been investigated in detail. In this study, alpha-fetoprotein (AFP) was used as a target biomolecule. The signals for AFP solutions of various concentrations, or with interfering materials, were detected via IMR. These samples were also used for characterizing the detection threshold and interference with enzyme-linked immunosorbent assay (ELISA). The results of assaying AFP level with IMR and ELISA were compared. The detection threshold for assaying AFP with IMR was found to be 3 ng/mL, which is 15 times lower than that of ELISA, and definitely suppresses false negative. For the interfering materials noted commonly in serum such as hemoglobin, bilirubin, triglyceride, and vascular endothelial growth factor, there was no detectable interfering effect when assaying AFP with IMR. Several serum samples from normal people and liver-tumor-bearing patients were used for the detections of AFP concentration via IMR. These results reveal the feasibilities of assaying AFP in blood using IMR, as well as achieving high-sensitive and high-specific assay for AFP.Keywords: immunomagnetic reduction, ELISA, biofunctionalized magnetic nanoparticlesHuang KWYang SYHong YWChieh JJYang CCHorng HEWu CCHong CYYang HCDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1991-1996 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Huang KW
Yang SY
Hong YW
Chieh JJ
Yang CC
Horng HE
Wu CC
Hong CY
Yang HC
Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles
description Kai-Wen Huang1, Shieh-Yueh Yang2,3, Yu-Wei Hong3, Jen-Jie Chieh3, Che-Chuan Yang3, Herng-Er Horng3, Chau-Chung Wu4, Chin-Yih Hong5, Hong-Chang Yang61Department of Surgery and Hepatitis Research Center, National Taiwan University Hospital, National Taiwan University, Taipei, 2MagQu Co, Ltd, Sindian Dist, New Taipei City, 3Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei, 4Departments of Internal Medicine and Primary Care Medicine, College of Medicine, National Taiwan University, Taipei, 5Graduate Institute of Bio-medical Engineering, National Chung Hsing University, Taichung, 6Department of Physics, National Taiwan University, Taipei, TaiwanAbstract: Some previous reports have already shown the characterizations of immunomagnetic reduction (IMR). The assay technology involves the utilities of biofunctionalized magnetic nanoparticles to label target biomolecules. However, the detection threshold and interference tests for IMR have not been investigated in detail. In this study, alpha-fetoprotein (AFP) was used as a target biomolecule. The signals for AFP solutions of various concentrations, or with interfering materials, were detected via IMR. These samples were also used for characterizing the detection threshold and interference with enzyme-linked immunosorbent assay (ELISA). The results of assaying AFP level with IMR and ELISA were compared. The detection threshold for assaying AFP with IMR was found to be 3 ng/mL, which is 15 times lower than that of ELISA, and definitely suppresses false negative. For the interfering materials noted commonly in serum such as hemoglobin, bilirubin, triglyceride, and vascular endothelial growth factor, there was no detectable interfering effect when assaying AFP with IMR. Several serum samples from normal people and liver-tumor-bearing patients were used for the detections of AFP concentration via IMR. These results reveal the feasibilities of assaying AFP in blood using IMR, as well as achieving high-sensitive and high-specific assay for AFP.Keywords: immunomagnetic reduction, ELISA, biofunctionalized magnetic nanoparticles
format article
author Huang KW
Yang SY
Hong YW
Chieh JJ
Yang CC
Horng HE
Wu CC
Hong CY
Yang HC
author_facet Huang KW
Yang SY
Hong YW
Chieh JJ
Yang CC
Horng HE
Wu CC
Hong CY
Yang HC
author_sort Huang KW
title Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles
title_short Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles
title_full Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles
title_fullStr Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles
title_full_unstemmed Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles
title_sort feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/6f132fbf43be43ae9cf76f958278defa
work_keys_str_mv AT huangkw feasibilitystudiesforassayingalphafetoproteinusingantibodyactivatedmagneticnanoparticles
AT yangsy feasibilitystudiesforassayingalphafetoproteinusingantibodyactivatedmagneticnanoparticles
AT hongyw feasibilitystudiesforassayingalphafetoproteinusingantibodyactivatedmagneticnanoparticles
AT chiehjj feasibilitystudiesforassayingalphafetoproteinusingantibodyactivatedmagneticnanoparticles
AT yangcc feasibilitystudiesforassayingalphafetoproteinusingantibodyactivatedmagneticnanoparticles
AT hornghe feasibilitystudiesforassayingalphafetoproteinusingantibodyactivatedmagneticnanoparticles
AT wucc feasibilitystudiesforassayingalphafetoproteinusingantibodyactivatedmagneticnanoparticles
AT hongcy feasibilitystudiesforassayingalphafetoproteinusingantibodyactivatedmagneticnanoparticles
AT yanghc feasibilitystudiesforassayingalphafetoproteinusingantibodyactivatedmagneticnanoparticles
_version_ 1718402225496129536