Extraction of high quality DNA from seized Moroccan cannabis resin (Hashish).

The extraction and purification of nucleic acids is the first step in most molecular biology analysis techniques. The objective of this work is to obtain highly purified nucleic acids derived from Cannabis sativa resin seizure in order to conduct a DNA typing method for the individualization of cann...

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Autores principales:	Moulay Abdelaziz El Alaoui, Marouane Melloul, Sanaâ Alaoui Amine, Hamid Stambouli, Aziz El Bouri, Abdelmajid Soulaymani, Elmostafa El Fahime
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6f17d8534b2244a3b9a6b7bc97fd4f1c
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spelling	oai:doaj.org-article:6f17d8534b2244a3b9a6b7bc97fd4f1c2021-11-18T08:52:34ZExtraction of high quality DNA from seized Moroccan cannabis resin (Hashish).1932-620310.1371/journal.pone.0074714https://doaj.org/article/6f17d8534b2244a3b9a6b7bc97fd4f1c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24124454/?tool=EBIhttps://doaj.org/toc/1932-6203The extraction and purification of nucleic acids is the first step in most molecular biology analysis techniques. The objective of this work is to obtain highly purified nucleic acids derived from Cannabis sativa resin seizure in order to conduct a DNA typing method for the individualization of cannabis resin samples. To obtain highly purified nucleic acids from cannabis resin (Hashish) free from contaminants that cause inhibition of PCR reaction, we have tested two protocols: the CTAB protocol of Wagner and a CTAB protocol described by Somma (2004) adapted for difficult matrix. We obtained high quality genomic DNA from 8 cannabis resin seizures using the adapted protocol. DNA extracted by the Wagner CTAB protocol failed to give polymerase chain reaction (PCR) amplification of tetrahydrocannabinolic acid (THCA) synthase coding gene. However, the extracted DNA by the second protocol permits amplification of THCA synthase coding gene using different sets of primers as assessed by PCR. We describe here for the first time the possibility of DNA extraction from (Hashish) resin derived from Cannabis sativa. This allows the use of DNA molecular tests under special forensic circumstances.Moulay Abdelaziz El AlaouiMarouane MelloulSanaâ Alaoui AmineHamid StambouliAziz El BouriAbdelmajid SoulaymaniElmostafa El FahimePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e74714 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Moulay Abdelaziz El Alaoui Marouane Melloul Sanaâ Alaoui Amine Hamid Stambouli Aziz El Bouri Abdelmajid Soulaymani Elmostafa El Fahime Extraction of high quality DNA from seized Moroccan cannabis resin (Hashish).
description	The extraction and purification of nucleic acids is the first step in most molecular biology analysis techniques. The objective of this work is to obtain highly purified nucleic acids derived from Cannabis sativa resin seizure in order to conduct a DNA typing method for the individualization of cannabis resin samples. To obtain highly purified nucleic acids from cannabis resin (Hashish) free from contaminants that cause inhibition of PCR reaction, we have tested two protocols: the CTAB protocol of Wagner and a CTAB protocol described by Somma (2004) adapted for difficult matrix. We obtained high quality genomic DNA from 8 cannabis resin seizures using the adapted protocol. DNA extracted by the Wagner CTAB protocol failed to give polymerase chain reaction (PCR) amplification of tetrahydrocannabinolic acid (THCA) synthase coding gene. However, the extracted DNA by the second protocol permits amplification of THCA synthase coding gene using different sets of primers as assessed by PCR. We describe here for the first time the possibility of DNA extraction from (Hashish) resin derived from Cannabis sativa. This allows the use of DNA molecular tests under special forensic circumstances.
format	article
author	Moulay Abdelaziz El Alaoui Marouane Melloul Sanaâ Alaoui Amine Hamid Stambouli Aziz El Bouri Abdelmajid Soulaymani Elmostafa El Fahime
author_facet	Moulay Abdelaziz El Alaoui Marouane Melloul Sanaâ Alaoui Amine Hamid Stambouli Aziz El Bouri Abdelmajid Soulaymani Elmostafa El Fahime
author_sort	Moulay Abdelaziz El Alaoui
title	Extraction of high quality DNA from seized Moroccan cannabis resin (Hashish).
title_short	Extraction of high quality DNA from seized Moroccan cannabis resin (Hashish).
title_full	Extraction of high quality DNA from seized Moroccan cannabis resin (Hashish).
title_fullStr	Extraction of high quality DNA from seized Moroccan cannabis resin (Hashish).
title_full_unstemmed	Extraction of high quality DNA from seized Moroccan cannabis resin (Hashish).
title_sort	extraction of high quality dna from seized moroccan cannabis resin (hashish).
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6f17d8534b2244a3b9a6b7bc97fd4f1c
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Extraction of high quality DNA from seized Moroccan cannabis resin (Hashish).

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