CD8+ lymphocytes control viral replication in SIVmac239-infected rhesus macaques without decreasing the lifespan of productively infected cells.

While CD8+ T cells are clearly important in controlling virus replication during HIV and SIV infections, the mechanisms underlying this antiviral effect remain poorly understood. In this study, we assessed the in vivo effect of CD8+ lymphocyte depletion on the lifespan of productively infected cells...

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Autores principales: Nichole R Klatt, Emi Shudo, Alex M Ortiz, Jessica C Engram, Mirko Paiardini, Benton Lawson, Michael D Miller, James Else, Ivona Pandrea, Jacob D Estes, Cristian Apetrei, Joern E Schmitz, Ruy M Ribeiro, Alan S Perelson, Guido Silvestri
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Publicado: Public Library of Science (PLoS) 2010
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spelling oai:doaj.org-article:6f185ff767e24750be4e4e2808b532712021-11-25T05:48:21ZCD8+ lymphocytes control viral replication in SIVmac239-infected rhesus macaques without decreasing the lifespan of productively infected cells.1553-73661553-737410.1371/journal.ppat.1000747https://doaj.org/article/6f185ff767e24750be4e4e2808b532712010-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20126441/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374While CD8+ T cells are clearly important in controlling virus replication during HIV and SIV infections, the mechanisms underlying this antiviral effect remain poorly understood. In this study, we assessed the in vivo effect of CD8+ lymphocyte depletion on the lifespan of productively infected cells during chronic SIVmac239 infection of rhesus macaques. We treated two groups of animals that were either CD8+ lymphocyte-depleted or controls with antiretroviral therapy, and used mathematical modeling to assess the lifespan of infected cells either in the presence or absence of CD8+ lymphocytes. We found that, in both early (day 57 post-SIV) and late (day 177 post-SIV) chronic SIV infection, depletion of CD8+ lymphocytes did not result in a measurable increase in the lifespan of either short- or long-lived productively infected cells in vivo. This result indicates that the presence of CD8+ lymphocytes does not result in a noticeably shorter lifespan of productively SIV-infected cells, and thus that direct cell killing is unlikely to be the main mechanism underlying the antiviral effect of CD8+ T cells in SIV-infected macaques with high virus replication.Nichole R KlattEmi ShudoAlex M OrtizJessica C EngramMirko PaiardiniBenton LawsonMichael D MillerJames ElseIvona PandreaJacob D EstesCristian ApetreiJoern E SchmitzRuy M RibeiroAlan S PerelsonGuido SilvestriPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 1, p e1000747 (2010)
institution DOAJ
collection DOAJ
language EN
topic Immunologic diseases. Allergy
RC581-607
Biology (General)
QH301-705.5
spellingShingle Immunologic diseases. Allergy
RC581-607
Biology (General)
QH301-705.5
Nichole R Klatt
Emi Shudo
Alex M Ortiz
Jessica C Engram
Mirko Paiardini
Benton Lawson
Michael D Miller
James Else
Ivona Pandrea
Jacob D Estes
Cristian Apetrei
Joern E Schmitz
Ruy M Ribeiro
Alan S Perelson
Guido Silvestri
CD8+ lymphocytes control viral replication in SIVmac239-infected rhesus macaques without decreasing the lifespan of productively infected cells.
description While CD8+ T cells are clearly important in controlling virus replication during HIV and SIV infections, the mechanisms underlying this antiviral effect remain poorly understood. In this study, we assessed the in vivo effect of CD8+ lymphocyte depletion on the lifespan of productively infected cells during chronic SIVmac239 infection of rhesus macaques. We treated two groups of animals that were either CD8+ lymphocyte-depleted or controls with antiretroviral therapy, and used mathematical modeling to assess the lifespan of infected cells either in the presence or absence of CD8+ lymphocytes. We found that, in both early (day 57 post-SIV) and late (day 177 post-SIV) chronic SIV infection, depletion of CD8+ lymphocytes did not result in a measurable increase in the lifespan of either short- or long-lived productively infected cells in vivo. This result indicates that the presence of CD8+ lymphocytes does not result in a noticeably shorter lifespan of productively SIV-infected cells, and thus that direct cell killing is unlikely to be the main mechanism underlying the antiviral effect of CD8+ T cells in SIV-infected macaques with high virus replication.
format article
author Nichole R Klatt
Emi Shudo
Alex M Ortiz
Jessica C Engram
Mirko Paiardini
Benton Lawson
Michael D Miller
James Else
Ivona Pandrea
Jacob D Estes
Cristian Apetrei
Joern E Schmitz
Ruy M Ribeiro
Alan S Perelson
Guido Silvestri
author_facet Nichole R Klatt
Emi Shudo
Alex M Ortiz
Jessica C Engram
Mirko Paiardini
Benton Lawson
Michael D Miller
James Else
Ivona Pandrea
Jacob D Estes
Cristian Apetrei
Joern E Schmitz
Ruy M Ribeiro
Alan S Perelson
Guido Silvestri
author_sort Nichole R Klatt
title CD8+ lymphocytes control viral replication in SIVmac239-infected rhesus macaques without decreasing the lifespan of productively infected cells.
title_short CD8+ lymphocytes control viral replication in SIVmac239-infected rhesus macaques without decreasing the lifespan of productively infected cells.
title_full CD8+ lymphocytes control viral replication in SIVmac239-infected rhesus macaques without decreasing the lifespan of productively infected cells.
title_fullStr CD8+ lymphocytes control viral replication in SIVmac239-infected rhesus macaques without decreasing the lifespan of productively infected cells.
title_full_unstemmed CD8+ lymphocytes control viral replication in SIVmac239-infected rhesus macaques without decreasing the lifespan of productively infected cells.
title_sort cd8+ lymphocytes control viral replication in sivmac239-infected rhesus macaques without decreasing the lifespan of productively infected cells.
publisher Public Library of Science (PLoS)
publishDate 2010
url https://doaj.org/article/6f185ff767e24750be4e4e2808b53271
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