A LbL-Assembled Bioactive Coating Modified Nanofibrous Membrane for Rapid Tendon-Bone Healing in ACL Reconstruction

Fei Han,1 Peng Zhang,2 Tianwu Chen,2 Chao Lin,1 Xuejun Wen,1 Peng Zhao1 1Institute for Translational Medicine, Institute for Biomedical Engineering and Nanoscience, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200092, People’s Republic of China; 2Department of Sp...

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Autores principales:	Han F, Zhang P, Chen T, Lin C, Wen X, Zhao P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	anterior cruciate ligament reconstruction tendon-bone healing layer-by-layer self-assembly nanofibrous membrane stromal derived factor-1α bone morphogenetic protein-2. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6f1b205670054afd935fb83a159706d9
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spelling	oai:doaj.org-article:6f1b205670054afd935fb83a159706d92021-12-02T09:39:41ZA LbL-Assembled Bioactive Coating Modified Nanofibrous Membrane for Rapid Tendon-Bone Healing in ACL Reconstruction1178-2013https://doaj.org/article/6f1b205670054afd935fb83a159706d92019-11-01T00:00:00Zhttps://www.dovepress.com/a-lbl-assembled-bioactive-coating-modified-nanofibrous-membrane-for-ra-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fei Han,1 Peng Zhang,2 Tianwu Chen,2 Chao Lin,1 Xuejun Wen,1 Peng Zhao1 1Institute for Translational Medicine, Institute for Biomedical Engineering and Nanoscience, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200092, People’s Republic of China; 2Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, People’s Republic of ChinaCorrespondence: Peng Zhao; Peng ZhangInstitute for Translational Medicine, Institute for Biomedical Engineering and Nanoscience, Shanghai East Hospital, Tongji University School of Medicine, Tongji University, Room 222, Shixun Building, Shanghai 200092, People’s Republic of ChinaTel +86 21 65988029Fax +86 21 65983708-0Email zp@tongji.edu.cn; zhangpengym@163.comIntroduction: In anterior cruciate ligament (ACL) reconstruction, hamstring tendon autograft is a well-accepted surgical choice as an alternative ACL graft. But the main disadvantage of autograft is its inefficient healing with host bone-tunnel which will leading to surgery failure.Methods: A biomimetic nanofibrous membrane for tendon-bone integration is fabricated in this work, which is composed of polycaprolactone (PCL) electrospinning membrane and chitosan/hyaluronic acid (CS/HA) multilayers film.Results: By using layer-by-layer (LbL) self-assembly this functional CS/HA multilayer films are deposited on the surface of PCL nanofiber to enable the local delivery of stromal cell-derived factor-1 α (SDF-1α) and bone morphogenetic protein-2 (BMP-2) in tendon-bone interface. This membrane can promote cell proliferation and recruitment, as well as inducing the osteogenic differentiation and recruitment of BMSCs.Conclusion: Further in vivo studies demonstrate that to wrap the tendon autograft using the membrane may afford superior tendon-bone integration and inhibit scar tissue formation in a rabbit ACL reconstruction model. More importantly, the biomechanical properties of the tendon-bone interface have been improved. This study shows that this biomimetic nanofibrous membrane is effective for improving tendon-bone healing after ACL reconstruction surgery.Keywords: anterior cruciate ligament reconstruction, tendon-bone healing, layer-by-layer self-assembly, nanofibrous membrane, stromal derived factor-1αHan FZhang PChen TLin CWen XZhao PDove Medical Pressarticleanterior cruciate ligament reconstructiontendon-bone healinglayer-by-layer self-assemblynanofibrous membranestromal derived factor-1αbone morphogenetic protein-2.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 9159-9172 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	anterior cruciate ligament reconstruction tendon-bone healing layer-by-layer self-assembly nanofibrous membrane stromal derived factor-1α bone morphogenetic protein-2. Medicine (General) R5-920
spellingShingle	anterior cruciate ligament reconstruction tendon-bone healing layer-by-layer self-assembly nanofibrous membrane stromal derived factor-1α bone morphogenetic protein-2. Medicine (General) R5-920 Han F Zhang P Chen T Lin C Wen X Zhao P A LbL-Assembled Bioactive Coating Modified Nanofibrous Membrane for Rapid Tendon-Bone Healing in ACL Reconstruction
description	Fei Han,1 Peng Zhang,2 Tianwu Chen,2 Chao Lin,1 Xuejun Wen,1 Peng Zhao1 1Institute for Translational Medicine, Institute for Biomedical Engineering and Nanoscience, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200092, People’s Republic of China; 2Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, People’s Republic of ChinaCorrespondence: Peng Zhao; Peng ZhangInstitute for Translational Medicine, Institute for Biomedical Engineering and Nanoscience, Shanghai East Hospital, Tongji University School of Medicine, Tongji University, Room 222, Shixun Building, Shanghai 200092, People’s Republic of ChinaTel +86 21 65988029Fax +86 21 65983708-0Email zp@tongji.edu.cn; zhangpengym@163.comIntroduction: In anterior cruciate ligament (ACL) reconstruction, hamstring tendon autograft is a well-accepted surgical choice as an alternative ACL graft. But the main disadvantage of autograft is its inefficient healing with host bone-tunnel which will leading to surgery failure.Methods: A biomimetic nanofibrous membrane for tendon-bone integration is fabricated in this work, which is composed of polycaprolactone (PCL) electrospinning membrane and chitosan/hyaluronic acid (CS/HA) multilayers film.Results: By using layer-by-layer (LbL) self-assembly this functional CS/HA multilayer films are deposited on the surface of PCL nanofiber to enable the local delivery of stromal cell-derived factor-1 α (SDF-1α) and bone morphogenetic protein-2 (BMP-2) in tendon-bone interface. This membrane can promote cell proliferation and recruitment, as well as inducing the osteogenic differentiation and recruitment of BMSCs.Conclusion: Further in vivo studies demonstrate that to wrap the tendon autograft using the membrane may afford superior tendon-bone integration and inhibit scar tissue formation in a rabbit ACL reconstruction model. More importantly, the biomechanical properties of the tendon-bone interface have been improved. This study shows that this biomimetic nanofibrous membrane is effective for improving tendon-bone healing after ACL reconstruction surgery.Keywords: anterior cruciate ligament reconstruction, tendon-bone healing, layer-by-layer self-assembly, nanofibrous membrane, stromal derived factor-1α
format	article
author	Han F Zhang P Chen T Lin C Wen X Zhao P
author_facet	Han F Zhang P Chen T Lin C Wen X Zhao P
author_sort	Han F
title	A LbL-Assembled Bioactive Coating Modified Nanofibrous Membrane for Rapid Tendon-Bone Healing in ACL Reconstruction
title_short	A LbL-Assembled Bioactive Coating Modified Nanofibrous Membrane for Rapid Tendon-Bone Healing in ACL Reconstruction
title_full	A LbL-Assembled Bioactive Coating Modified Nanofibrous Membrane for Rapid Tendon-Bone Healing in ACL Reconstruction
title_fullStr	A LbL-Assembled Bioactive Coating Modified Nanofibrous Membrane for Rapid Tendon-Bone Healing in ACL Reconstruction
title_full_unstemmed	A LbL-Assembled Bioactive Coating Modified Nanofibrous Membrane for Rapid Tendon-Bone Healing in ACL Reconstruction
title_sort	lbl-assembled bioactive coating modified nanofibrous membrane for rapid tendon-bone healing in acl reconstruction
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/6f1b205670054afd935fb83a159706d9
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A LbL-Assembled Bioactive Coating Modified Nanofibrous Membrane for Rapid Tendon-Bone Healing in ACL Reconstruction

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