Accelerative action of topical piperonylic acid on mice full thickness wound by modulating inflammation and collagen deposition

Epidermal growth factor (EGF) promotes cell growth, proliferation, and survival in numerous tissues. Piperonylic acid, a metabolite present in peppers (Piper nigrum L. and Piper longum L.), can bind to the epidermal growth factor receptor (EGFR) and induce an intracellular signaling cascade leading...

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Autores principales:	Karina Gomes Moreira, Thais Paulino do Prado, Natália Ferreira Mendes, Renan de Medeiros Bezerra, Carlos Poblete Jara, Maria Helena Melo Lima, Eliana Pereira de Araujo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6f1c9cad95ce4cabbc9a0d296a944dcb
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spelling	oai:doaj.org-article:6f1c9cad95ce4cabbc9a0d296a944dcb2021-11-04T06:19:42ZAccelerative action of topical piperonylic acid on mice full thickness wound by modulating inflammation and collagen deposition1932-6203https://doaj.org/article/6f1c9cad95ce4cabbc9a0d296a944dcb2021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8547657/?tool=EBIhttps://doaj.org/toc/1932-6203Epidermal growth factor (EGF) promotes cell growth, proliferation, and survival in numerous tissues. Piperonylic acid, a metabolite present in peppers (Piper nigrum L. and Piper longum L.), can bind to the epidermal growth factor receptor (EGFR) and induce an intracellular signaling cascade leading to the transcription of genes responsible for these actions, especially in keratinocytes. These cells are fundamental in maintaining cutaneous homeostasis and are the first to be damaged in the case of a wound. Thus, we hypothesized that piperonylic acid improves wound healing. C57BL6/J male mice were submitted to dorsal skin wounds caused by a 6 mm punch and treated topically with piperonylic acid or vehicle. The wounds were evaluated macro- and microscopically, and tissue samples were collected for immunofluorescence and real-time PCR analyses on days 6, 9 and 19 post-injury. Topical piperonylic acid improved wound healing from day 6 post-injury until closure. This phenomenon apparently occurred through EGFR activation. In addition, piperonylic acid modulated the gene expression of interleukin (Il)-6, il-1β, tumor necrosis factor (Tnf)-α, il-10, monocyte chemoattractant protein (Mcp)-1 and insulin-like growth factor (Igf)-1, which are important for the healing process. By day 19 post-injury, the new tissue showed greater deposition of type I collagen and a morphology closer to intact skin, with more dermal papillae and hair follicles. We conclude that piperonylic acid may be a viable option for the treatment of skin wounds.Karina Gomes MoreiraThais Paulino do PradoNatália Ferreira MendesRenan de Medeiros BezerraCarlos Poblete JaraMaria Helena Melo LimaEliana Pereira de AraujoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karina Gomes Moreira Thais Paulino do Prado Natália Ferreira Mendes Renan de Medeiros Bezerra Carlos Poblete Jara Maria Helena Melo Lima Eliana Pereira de Araujo Accelerative action of topical piperonylic acid on mice full thickness wound by modulating inflammation and collagen deposition
description	Epidermal growth factor (EGF) promotes cell growth, proliferation, and survival in numerous tissues. Piperonylic acid, a metabolite present in peppers (Piper nigrum L. and Piper longum L.), can bind to the epidermal growth factor receptor (EGFR) and induce an intracellular signaling cascade leading to the transcription of genes responsible for these actions, especially in keratinocytes. These cells are fundamental in maintaining cutaneous homeostasis and are the first to be damaged in the case of a wound. Thus, we hypothesized that piperonylic acid improves wound healing. C57BL6/J male mice were submitted to dorsal skin wounds caused by a 6 mm punch and treated topically with piperonylic acid or vehicle. The wounds were evaluated macro- and microscopically, and tissue samples were collected for immunofluorescence and real-time PCR analyses on days 6, 9 and 19 post-injury. Topical piperonylic acid improved wound healing from day 6 post-injury until closure. This phenomenon apparently occurred through EGFR activation. In addition, piperonylic acid modulated the gene expression of interleukin (Il)-6, il-1β, tumor necrosis factor (Tnf)-α, il-10, monocyte chemoattractant protein (Mcp)-1 and insulin-like growth factor (Igf)-1, which are important for the healing process. By day 19 post-injury, the new tissue showed greater deposition of type I collagen and a morphology closer to intact skin, with more dermal papillae and hair follicles. We conclude that piperonylic acid may be a viable option for the treatment of skin wounds.
format	article
author	Karina Gomes Moreira Thais Paulino do Prado Natália Ferreira Mendes Renan de Medeiros Bezerra Carlos Poblete Jara Maria Helena Melo Lima Eliana Pereira de Araujo
author_facet	Karina Gomes Moreira Thais Paulino do Prado Natália Ferreira Mendes Renan de Medeiros Bezerra Carlos Poblete Jara Maria Helena Melo Lima Eliana Pereira de Araujo
author_sort	Karina Gomes Moreira
title	Accelerative action of topical piperonylic acid on mice full thickness wound by modulating inflammation and collagen deposition
title_short	Accelerative action of topical piperonylic acid on mice full thickness wound by modulating inflammation and collagen deposition
title_full	Accelerative action of topical piperonylic acid on mice full thickness wound by modulating inflammation and collagen deposition
title_fullStr	Accelerative action of topical piperonylic acid on mice full thickness wound by modulating inflammation and collagen deposition
title_full_unstemmed	Accelerative action of topical piperonylic acid on mice full thickness wound by modulating inflammation and collagen deposition
title_sort	accelerative action of topical piperonylic acid on mice full thickness wound by modulating inflammation and collagen deposition
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6f1c9cad95ce4cabbc9a0d296a944dcb
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Accelerative action of topical piperonylic acid on mice full thickness wound by modulating inflammation and collagen deposition

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