Enterotoxin and Emetic Toxin Genes Profiles and Genetic Diversity of Bacillus cereus Isolated from Food, Environmental and Clinical Samples in Serbia

Bacillus cereus, usually ingested by food, can cause two types of disease due to the presence of toxins: vomiting and diarrhea syndrome. Systemic infections can also occur. The aim was to detect genes for enterotoxins (hblA, entFM) and emetic toxin (cer) and to investigate the genetic heterogeneity...

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Autores principales:	Dejana Savić, Elizabeta Ristanović, Biljana Miljković Selimović, Sonja Radaković, Dragana Jošić, Zorica Lepšanović
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2020
Materias:	bacillus cereus enterotoxin genes emetic toxin gene pcr rapd typing Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/6f203b61f72f4f61a49a290c8c209959
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spelling	oai:doaj.org-article:6f203b61f72f4f61a49a290c8c2099592021-11-17T21:27:53ZEnterotoxin and Emetic Toxin Genes Profiles and Genetic Diversity of Bacillus cereus Isolated from Food, Environmental and Clinical Samples in Serbia1820-744810.2478/acve-2020-0013https://doaj.org/article/6f203b61f72f4f61a49a290c8c2099592020-06-01T00:00:00Zhttps://doi.org/10.2478/acve-2020-0013https://doaj.org/toc/1820-7448Bacillus cereus, usually ingested by food, can cause two types of disease due to the presence of toxins: vomiting and diarrhea syndrome. Systemic infections can also occur. The aim was to detect genes for enterotoxins (hblA, entFM) and emetic toxin (cer) and to investigate the genetic heterogeneity of B. cereus isolates from food, environment and human stool. Identification of B. cereus was performed by means of selective medium, classical biochemical test and polymerase chain reaction (PCR). Toxin genes were detected by PCR. Typing was performed by random amplified polymorphic DNA (RAPD). EntFM gene was present in all stool and food samples and in 28/30 environmental isolates. HblA gene was present in 29/30 stool, 23/30 food and 24/30 environmental isolates. Cer gene was present in 30/30 stool, 28/30 food and 25/30 environmental isolates. The RAPD results show high heterogeneity among the isolates from each group. In the cumulative dendrogram, representative isolates from all three groups formed two clusters with a difference of 53%. The detection of toxin genes in all B. cereus isolates indicated these bacteria as potentially pathogenic and a serious threat for human health. The presence of isolates from all three groups in the same cluster suggests the existence of similar strains in the environment, food and patients, which is in line with the circulation of strains in nature through the food chain.Dejana SavićElizabeta RistanovićBiljana Miljković SelimovićSonja RadakovićDragana JošićZorica LepšanovićSciendoarticlebacillus cereusenterotoxin genesemetic toxin genepcrrapd typingVeterinary medicineSF600-1100ENActa Veterinaria, Vol 70, Iss 2, Pp 182-193 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacillus cereus enterotoxin genes emetic toxin gene pcr rapd typing Veterinary medicine SF600-1100
spellingShingle	bacillus cereus enterotoxin genes emetic toxin gene pcr rapd typing Veterinary medicine SF600-1100 Dejana Savić Elizabeta Ristanović Biljana Miljković Selimović Sonja Radaković Dragana Jošić Zorica Lepšanović Enterotoxin and Emetic Toxin Genes Profiles and Genetic Diversity of Bacillus cereus Isolated from Food, Environmental and Clinical Samples in Serbia
description	Bacillus cereus, usually ingested by food, can cause two types of disease due to the presence of toxins: vomiting and diarrhea syndrome. Systemic infections can also occur. The aim was to detect genes for enterotoxins (hblA, entFM) and emetic toxin (cer) and to investigate the genetic heterogeneity of B. cereus isolates from food, environment and human stool. Identification of B. cereus was performed by means of selective medium, classical biochemical test and polymerase chain reaction (PCR). Toxin genes were detected by PCR. Typing was performed by random amplified polymorphic DNA (RAPD). EntFM gene was present in all stool and food samples and in 28/30 environmental isolates. HblA gene was present in 29/30 stool, 23/30 food and 24/30 environmental isolates. Cer gene was present in 30/30 stool, 28/30 food and 25/30 environmental isolates. The RAPD results show high heterogeneity among the isolates from each group. In the cumulative dendrogram, representative isolates from all three groups formed two clusters with a difference of 53%. The detection of toxin genes in all B. cereus isolates indicated these bacteria as potentially pathogenic and a serious threat for human health. The presence of isolates from all three groups in the same cluster suggests the existence of similar strains in the environment, food and patients, which is in line with the circulation of strains in nature through the food chain.
format	article
author	Dejana Savić Elizabeta Ristanović Biljana Miljković Selimović Sonja Radaković Dragana Jošić Zorica Lepšanović
author_facet	Dejana Savić Elizabeta Ristanović Biljana Miljković Selimović Sonja Radaković Dragana Jošić Zorica Lepšanović
author_sort	Dejana Savić
title	Enterotoxin and Emetic Toxin Genes Profiles and Genetic Diversity of Bacillus cereus Isolated from Food, Environmental and Clinical Samples in Serbia
title_short	Enterotoxin and Emetic Toxin Genes Profiles and Genetic Diversity of Bacillus cereus Isolated from Food, Environmental and Clinical Samples in Serbia
title_full	Enterotoxin and Emetic Toxin Genes Profiles and Genetic Diversity of Bacillus cereus Isolated from Food, Environmental and Clinical Samples in Serbia
title_fullStr	Enterotoxin and Emetic Toxin Genes Profiles and Genetic Diversity of Bacillus cereus Isolated from Food, Environmental and Clinical Samples in Serbia
title_full_unstemmed	Enterotoxin and Emetic Toxin Genes Profiles and Genetic Diversity of Bacillus cereus Isolated from Food, Environmental and Clinical Samples in Serbia
title_sort	enterotoxin and emetic toxin genes profiles and genetic diversity of bacillus cereus isolated from food, environmental and clinical samples in serbia
publisher	Sciendo
publishDate	2020
url	https://doaj.org/article/6f203b61f72f4f61a49a290c8c209959
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Enterotoxin and Emetic Toxin Genes Profiles and Genetic Diversity of Bacillus cereus Isolated from Food, Environmental and Clinical Samples in Serbia

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