Comparative susceptibility of different biological forms of Anopheles stephensi to Plasmodium berghei ANKA strain.

<h4>Background</h4>There are varying degrees of compatibility between malaria parasite-mosquito species, and understanding this compatibility may be crucial for developing effective transmission-blocking vaccines. This study investigates the compatibility of different biological forms of...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Hamid R Basseri, Habib Mohamadzadeh Hajipirloo, Mulood Mohammadi Bavani, Miranda M A Whitten
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2013
Materias:
R
Q
Acceso en línea:https://doaj.org/article/6f253c2607e4438eb97f7ade0341ed28
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:6f253c2607e4438eb97f7ade0341ed28
record_format dspace
spelling oai:doaj.org-article:6f253c2607e4438eb97f7ade0341ed282021-11-18T08:54:11ZComparative susceptibility of different biological forms of Anopheles stephensi to Plasmodium berghei ANKA strain.1932-620310.1371/journal.pone.0075413https://doaj.org/article/6f253c2607e4438eb97f7ade0341ed282013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24086525/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>There are varying degrees of compatibility between malaria parasite-mosquito species, and understanding this compatibility may be crucial for developing effective transmission-blocking vaccines. This study investigates the compatibility of different biological forms of a malaria vector, Anopheles stephensi, to Plasmodium berghei ANKA strain.<h4>Methods</h4>Several biologically different and allopatric forms of A. stephensi were studied. Three forms were isolated from different regions of southern Iran: the variety mysorensis, the intermediate form and the native type form, and an additional type form originated from India (Beech strain).The mosquitoes were experimentally infected with P. berghei to compare their susceptibility to parasitism. Anti-mosquito midgut antiserum was then raised in BALB/cs mice immunized against gut antigens from the most susceptible form of A. stephensi (Beech strain), and the efficacy of the antiserum was assessed in transmission-blocking assays conducted on the least susceptible mosquito biological form.<h4>Results</h4>The susceptibility of different biological forms of A. stephensi mosquito to P. berghei was specifically inter-type varied. The Beech strain and the intermediate form were both highly susceptible to infection, with higher oocyst and sporozoite infection rates than intermediate and mysorensis forms. The oocyst infection, and particularly sporozite infection, was lowest in the mysorensis strain. Antiserum raised against midgut proteins of the Indian Beech type form blocked infection in this mosquito population, but it was ineffective at blocking both oocyst and sporozoite development in the permissive but geographically distant intermediate form mosquitoes. This suggests that a strong degree of incompatibility exists between the mosquito strains in terms of midgut protein(s) acting as putative ookinete receptors.<h4>Conclusions</h4>The incompatibility in the midgut protein profiles between two biological forms of A. stephensi demonstrates a well-differentiated population structure according to geographical origin. Therefore, the design of potential transmission-blocking strategies should incorporate a more thorough understanding of intra-species variations in host-parasite interactions.Hamid R BasseriHabib Mohamadzadeh HajipirlooMulood Mohammadi BavaniMiranda M A WhittenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e75413 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Hamid R Basseri
Habib Mohamadzadeh Hajipirloo
Mulood Mohammadi Bavani
Miranda M A Whitten
Comparative susceptibility of different biological forms of Anopheles stephensi to Plasmodium berghei ANKA strain.
description <h4>Background</h4>There are varying degrees of compatibility between malaria parasite-mosquito species, and understanding this compatibility may be crucial for developing effective transmission-blocking vaccines. This study investigates the compatibility of different biological forms of a malaria vector, Anopheles stephensi, to Plasmodium berghei ANKA strain.<h4>Methods</h4>Several biologically different and allopatric forms of A. stephensi were studied. Three forms were isolated from different regions of southern Iran: the variety mysorensis, the intermediate form and the native type form, and an additional type form originated from India (Beech strain).The mosquitoes were experimentally infected with P. berghei to compare their susceptibility to parasitism. Anti-mosquito midgut antiserum was then raised in BALB/cs mice immunized against gut antigens from the most susceptible form of A. stephensi (Beech strain), and the efficacy of the antiserum was assessed in transmission-blocking assays conducted on the least susceptible mosquito biological form.<h4>Results</h4>The susceptibility of different biological forms of A. stephensi mosquito to P. berghei was specifically inter-type varied. The Beech strain and the intermediate form were both highly susceptible to infection, with higher oocyst and sporozoite infection rates than intermediate and mysorensis forms. The oocyst infection, and particularly sporozite infection, was lowest in the mysorensis strain. Antiserum raised against midgut proteins of the Indian Beech type form blocked infection in this mosquito population, but it was ineffective at blocking both oocyst and sporozoite development in the permissive but geographically distant intermediate form mosquitoes. This suggests that a strong degree of incompatibility exists between the mosquito strains in terms of midgut protein(s) acting as putative ookinete receptors.<h4>Conclusions</h4>The incompatibility in the midgut protein profiles between two biological forms of A. stephensi demonstrates a well-differentiated population structure according to geographical origin. Therefore, the design of potential transmission-blocking strategies should incorporate a more thorough understanding of intra-species variations in host-parasite interactions.
format article
author Hamid R Basseri
Habib Mohamadzadeh Hajipirloo
Mulood Mohammadi Bavani
Miranda M A Whitten
author_facet Hamid R Basseri
Habib Mohamadzadeh Hajipirloo
Mulood Mohammadi Bavani
Miranda M A Whitten
author_sort Hamid R Basseri
title Comparative susceptibility of different biological forms of Anopheles stephensi to Plasmodium berghei ANKA strain.
title_short Comparative susceptibility of different biological forms of Anopheles stephensi to Plasmodium berghei ANKA strain.
title_full Comparative susceptibility of different biological forms of Anopheles stephensi to Plasmodium berghei ANKA strain.
title_fullStr Comparative susceptibility of different biological forms of Anopheles stephensi to Plasmodium berghei ANKA strain.
title_full_unstemmed Comparative susceptibility of different biological forms of Anopheles stephensi to Plasmodium berghei ANKA strain.
title_sort comparative susceptibility of different biological forms of anopheles stephensi to plasmodium berghei anka strain.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/6f253c2607e4438eb97f7ade0341ed28
work_keys_str_mv AT hamidrbasseri comparativesusceptibilityofdifferentbiologicalformsofanophelesstephensitoplasmodiumbergheiankastrain
AT habibmohamadzadehhajipirloo comparativesusceptibilityofdifferentbiologicalformsofanophelesstephensitoplasmodiumbergheiankastrain
AT muloodmohammadibavani comparativesusceptibilityofdifferentbiologicalformsofanophelesstephensitoplasmodiumbergheiankastrain
AT mirandamawhitten comparativesusceptibilityofdifferentbiologicalformsofanophelesstephensitoplasmodiumbergheiankastrain
_version_ 1718421207214194688