Controlled Transdermal Iontophoresis of Insulin from Water-Soluble Polypyrrole Nanoparticles: An In Vitro Study

The iontophoresis delivery of insulin (INS) remains a serious challenge due to the low permeability of the drug through the skin. This work aims to investigate the potential of water-soluble polypyrrole nanoparticles (WS-PPyNPs) as a drug donor matrix for controlled transdermal iontophoresis of INS....

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Autores principales: Kamran Tari, Soroush Khamoushian, Tayyebeh Madrakian, Abbas Afkhami, Marek Jan Łos, Arash Ghoorchian, Mohammad Reza Samarghandi, Saeid Ghavami
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spelling oai:doaj.org-article:6f3561134af2493ca99f517be483678f2021-11-25T17:57:02ZControlled Transdermal Iontophoresis of Insulin from Water-Soluble Polypyrrole Nanoparticles: An In Vitro Study10.3390/ijms2222124791422-00671661-6596https://doaj.org/article/6f3561134af2493ca99f517be483678f2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12479https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The iontophoresis delivery of insulin (INS) remains a serious challenge due to the low permeability of the drug through the skin. This work aims to investigate the potential of water-soluble polypyrrole nanoparticles (WS-PPyNPs) as a drug donor matrix for controlled transdermal iontophoresis of INS. WS-PPyNPs have been prepared via a simple chemical polymerization in the presence of sodium dodecyl sulfate (SDS) as both dopant and the stabilizing agent. The synthesis of the soluble polymer was characterized using field emission scanning electron microscopy (FESEM), dynamic light scattering (DLS), fluorescence spectroscopy, and Fourier transform infrared (FT–IR) spectroscopy. The loading mechanism of INS onto the WS-PPyNPs is based on the fact that the drug molecules can be replaced with doped dodecyl sulfate. A two-compartment Franz-type diffusion cell was employed to study the effect of current density, formulation pH, INS concentration, and sodium chloride concentration on anodal iontophoresis (AIP) and cathodal iontophoresis (CIP) of INS across the rat skin. Both AIP and CIP delivery of INS using WS-PPyNPs were significantly increased compared to passive delivery. Furthermore, while the AIP experiment (60 min at 0.13 mA cm<sup>–2</sup>) show low cumulative drug permeation for INS (about 20.48 µg cm<sup>−2</sup>); the CIP stimulation exhibited a cumulative drug permeation of 68.29 µg cm<sup>−2</sup>. This improvement is due to the separation of positively charged WS-PPyNPs and negatively charged INS that has occurred in the presence of cathodal stimulation. The obtained results confirm the potential applicability of WS-PPyNPs as an effective approach in the development of controlled transdermal iontophoresis of INS.Kamran TariSoroush KhamoushianTayyebeh MadrakianAbbas AfkhamiMarek Jan ŁosArash GhoorchianMohammad Reza SamarghandiSaeid GhavamiMDPI AGarticletransdermal iontophoresisinsulinwater soluble polypyrroleanodal iontophoresiscathodal iontophoresisBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12479, p 12479 (2021)
institution DOAJ
collection DOAJ
language EN
topic transdermal iontophoresis
insulin
water soluble polypyrrole
anodal iontophoresis
cathodal iontophoresis
Biology (General)
QH301-705.5
Chemistry
QD1-999
spellingShingle transdermal iontophoresis
insulin
water soluble polypyrrole
anodal iontophoresis
cathodal iontophoresis
Biology (General)
QH301-705.5
Chemistry
QD1-999
Kamran Tari
Soroush Khamoushian
Tayyebeh Madrakian
Abbas Afkhami
Marek Jan Łos
Arash Ghoorchian
Mohammad Reza Samarghandi
Saeid Ghavami
Controlled Transdermal Iontophoresis of Insulin from Water-Soluble Polypyrrole Nanoparticles: An In Vitro Study
description The iontophoresis delivery of insulin (INS) remains a serious challenge due to the low permeability of the drug through the skin. This work aims to investigate the potential of water-soluble polypyrrole nanoparticles (WS-PPyNPs) as a drug donor matrix for controlled transdermal iontophoresis of INS. WS-PPyNPs have been prepared via a simple chemical polymerization in the presence of sodium dodecyl sulfate (SDS) as both dopant and the stabilizing agent. The synthesis of the soluble polymer was characterized using field emission scanning electron microscopy (FESEM), dynamic light scattering (DLS), fluorescence spectroscopy, and Fourier transform infrared (FT–IR) spectroscopy. The loading mechanism of INS onto the WS-PPyNPs is based on the fact that the drug molecules can be replaced with doped dodecyl sulfate. A two-compartment Franz-type diffusion cell was employed to study the effect of current density, formulation pH, INS concentration, and sodium chloride concentration on anodal iontophoresis (AIP) and cathodal iontophoresis (CIP) of INS across the rat skin. Both AIP and CIP delivery of INS using WS-PPyNPs were significantly increased compared to passive delivery. Furthermore, while the AIP experiment (60 min at 0.13 mA cm<sup>–2</sup>) show low cumulative drug permeation for INS (about 20.48 µg cm<sup>−2</sup>); the CIP stimulation exhibited a cumulative drug permeation of 68.29 µg cm<sup>−2</sup>. This improvement is due to the separation of positively charged WS-PPyNPs and negatively charged INS that has occurred in the presence of cathodal stimulation. The obtained results confirm the potential applicability of WS-PPyNPs as an effective approach in the development of controlled transdermal iontophoresis of INS.
format article
author Kamran Tari
Soroush Khamoushian
Tayyebeh Madrakian
Abbas Afkhami
Marek Jan Łos
Arash Ghoorchian
Mohammad Reza Samarghandi
Saeid Ghavami
author_facet Kamran Tari
Soroush Khamoushian
Tayyebeh Madrakian
Abbas Afkhami
Marek Jan Łos
Arash Ghoorchian
Mohammad Reza Samarghandi
Saeid Ghavami
author_sort Kamran Tari
title Controlled Transdermal Iontophoresis of Insulin from Water-Soluble Polypyrrole Nanoparticles: An In Vitro Study
title_short Controlled Transdermal Iontophoresis of Insulin from Water-Soluble Polypyrrole Nanoparticles: An In Vitro Study
title_full Controlled Transdermal Iontophoresis of Insulin from Water-Soluble Polypyrrole Nanoparticles: An In Vitro Study
title_fullStr Controlled Transdermal Iontophoresis of Insulin from Water-Soluble Polypyrrole Nanoparticles: An In Vitro Study
title_full_unstemmed Controlled Transdermal Iontophoresis of Insulin from Water-Soluble Polypyrrole Nanoparticles: An In Vitro Study
title_sort controlled transdermal iontophoresis of insulin from water-soluble polypyrrole nanoparticles: an in vitro study
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/6f3561134af2493ca99f517be483678f
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