Detachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces

Abstract Bacterial adhesion to surfaces occurs ubiquitously and is initially reversible, though becoming more irreversible within minutes after first contact with a surface. We here demonstrate for eight bacterial strains comprising four species, that bacteria adhere irreversibly to surfaces through...

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Autores principales:	Jelmer Sjollema, Henny C. van der Mei, Connie L. Hall, Brandon W. Peterson, Joop de Vries, Lei Song, Ed D. de Jong, Henk J. Busscher, Jan J. T. M. Swartjes
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6f3cd3100ddd4542af21227c208066f0
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spelling	oai:doaj.org-article:6f3cd3100ddd4542af21227c208066f02021-12-02T11:53:10ZDetachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces10.1038/s41598-017-04703-82045-2322https://doaj.org/article/6f3cd3100ddd4542af21227c208066f02017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04703-8https://doaj.org/toc/2045-2322Abstract Bacterial adhesion to surfaces occurs ubiquitously and is initially reversible, though becoming more irreversible within minutes after first contact with a surface. We here demonstrate for eight bacterial strains comprising four species, that bacteria adhere irreversibly to surfaces through multiple, reversibly-binding tethers that detach and successively re-attach, but not collectively detach to cause detachment of an entire bacterium. Arguments build on combining analyses of confined Brownian-motion of bacteria adhering to glass and their AFM force-distance curves and include the following observations: (1) force-distance curves showed detachment events indicative of multiple binding tethers, (2) vibration amplitudes of adhering bacteria parallel to a surface decreased with increasing adhesion-forces acting perpendicular to the surface, (3) nanoscopic displacements of bacteria with relatively long autocorrelation times up to several seconds, in absence of microscopic displacement, (4) increases in Mean-Squared-Displacement over prolonged time periods according to tα with 0 < α ≪ 1, indicative of confined displacement. Analysis of simulated position-maps of adhering particles using a new, in silico model confirmed that adhesion to surfaces is irreversible through detachment and successive re-attachment of reversibly-binding tethers. This makes bacterial adhesion mechanistically comparable with the irreversible adsorption of high-molecular-weight proteins to surfaces, mediated by multiple, reversibly-binding molecular segments.Jelmer SjollemaHenny C. van der MeiConnie L. HallBrandon W. PetersonJoop de VriesLei SongEd D. de JongHenk J. BusscherJan J. T. M. SwartjesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jelmer Sjollema Henny C. van der Mei Connie L. Hall Brandon W. Peterson Joop de Vries Lei Song Ed D. de Jong Henk J. Busscher Jan J. T. M. Swartjes Detachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces
description	Abstract Bacterial adhesion to surfaces occurs ubiquitously and is initially reversible, though becoming more irreversible within minutes after first contact with a surface. We here demonstrate for eight bacterial strains comprising four species, that bacteria adhere irreversibly to surfaces through multiple, reversibly-binding tethers that detach and successively re-attach, but not collectively detach to cause detachment of an entire bacterium. Arguments build on combining analyses of confined Brownian-motion of bacteria adhering to glass and their AFM force-distance curves and include the following observations: (1) force-distance curves showed detachment events indicative of multiple binding tethers, (2) vibration amplitudes of adhering bacteria parallel to a surface decreased with increasing adhesion-forces acting perpendicular to the surface, (3) nanoscopic displacements of bacteria with relatively long autocorrelation times up to several seconds, in absence of microscopic displacement, (4) increases in Mean-Squared-Displacement over prolonged time periods according to tα with 0 < α ≪ 1, indicative of confined displacement. Analysis of simulated position-maps of adhering particles using a new, in silico model confirmed that adhesion to surfaces is irreversible through detachment and successive re-attachment of reversibly-binding tethers. This makes bacterial adhesion mechanistically comparable with the irreversible adsorption of high-molecular-weight proteins to surfaces, mediated by multiple, reversibly-binding molecular segments.
format	article
author	Jelmer Sjollema Henny C. van der Mei Connie L. Hall Brandon W. Peterson Joop de Vries Lei Song Ed D. de Jong Henk J. Busscher Jan J. T. M. Swartjes
author_facet	Jelmer Sjollema Henny C. van der Mei Connie L. Hall Brandon W. Peterson Joop de Vries Lei Song Ed D. de Jong Henk J. Busscher Jan J. T. M. Swartjes
author_sort	Jelmer Sjollema
title	Detachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces
title_short	Detachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces
title_full	Detachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces
title_fullStr	Detachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces
title_full_unstemmed	Detachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces
title_sort	detachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6f3cd3100ddd4542af21227c208066f0
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Detachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces

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