Estimation of the density of neural, glial, and endothelial lineage cells in the adult mouse dentate gyrus

The dentate gyrus subregion of the mammalian hippocampus is an adult neural stem cell niche and site of lifelong neurogenesis. Hypotheses regarding the role of adult-born neuron synaptic integration in hippocampal circuit function are framed by robust estimations of adult-born versus pre/perinatally...

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Autores principales:	Joshua D Rieskamp, Patricia Sarchet, Bryon M Smith, Elizabeth D Kirby
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2022
Materias:	adult neurogenesis; dentate gyrus; endothelia; glia; hippocampus; neural stem cell; secretome; stereology Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/6f418ba0229d466fa6fa04784accc2f2
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spelling	oai:doaj.org-article:6f418ba0229d466fa6fa04784accc2f22021-11-19T12:16:44ZEstimation of the density of neural, glial, and endothelial lineage cells in the adult mouse dentate gyrus1673-537410.4103/1673-5374.327354https://doaj.org/article/6f418ba0229d466fa6fa04784accc2f22022-01-01T00:00:00Zhttp://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=6;spage=1286;epage=1292;aulast=Rieskamphttps://doaj.org/toc/1673-5374The dentate gyrus subregion of the mammalian hippocampus is an adult neural stem cell niche and site of lifelong neurogenesis. Hypotheses regarding the role of adult-born neuron synaptic integration in hippocampal circuit function are framed by robust estimations of adult-born versus pre/perinatally-born neuron number. In contrast, the non-neurogenic functions of adult neural stem cells and their immediate progeny, such as secretion of bioactive growth factors and expression of extracellular matrix-modifying proteins, lack similar framing due to few estimates of their number versus other prominent secretory cells. Here, we apply immunohistochemical methods to estimate cell density of neural stem/progenitor cells versus other major classes of glial and endothelial cell types that are potentially secretory in the dentate gyrus of adult mice. Of the cell types quantified, we found that GFAP+SOX2+ stellate astrocytes were the most numerous, followed by CD31+ endothelia, GFAP–SOX2+ intermediate progenitors, Olig2+ oligodendrocytes, Iba1+ microglia, and GFAP+SOX2+ radial glia-like neural stem cells. We did not observe any significant sex differences in density of any cell population. Notably, neural stem/progenitor cells were present at a similar density as several cell types known to have potent functional roles via their secretome. These findings may be useful for refining hypotheses regarding the contributions of these cell types to regulating hippocampal function and their potential therapeutic uses. All experimental protocols were approved by the Ohio State University Institutional Animal Care and Use Committee (protocol# 2016A00000068) on July 14, 2016.Joshua D RieskampPatricia SarchetBryon M SmithElizabeth D KirbyWolters Kluwer Medknow Publicationsarticleadult neurogenesis; dentate gyrus; endothelia; glia; hippocampus; neural stem cell; secretome; stereologyNeurology. Diseases of the nervous systemRC346-429ENNeural Regeneration Research, Vol 17, Iss 6, Pp 1286-1292 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	adult neurogenesis; dentate gyrus; endothelia; glia; hippocampus; neural stem cell; secretome; stereology Neurology. Diseases of the nervous system RC346-429
spellingShingle	adult neurogenesis; dentate gyrus; endothelia; glia; hippocampus; neural stem cell; secretome; stereology Neurology. Diseases of the nervous system RC346-429 Joshua D Rieskamp Patricia Sarchet Bryon M Smith Elizabeth D Kirby Estimation of the density of neural, glial, and endothelial lineage cells in the adult mouse dentate gyrus
description	The dentate gyrus subregion of the mammalian hippocampus is an adult neural stem cell niche and site of lifelong neurogenesis. Hypotheses regarding the role of adult-born neuron synaptic integration in hippocampal circuit function are framed by robust estimations of adult-born versus pre/perinatally-born neuron number. In contrast, the non-neurogenic functions of adult neural stem cells and their immediate progeny, such as secretion of bioactive growth factors and expression of extracellular matrix-modifying proteins, lack similar framing due to few estimates of their number versus other prominent secretory cells. Here, we apply immunohistochemical methods to estimate cell density of neural stem/progenitor cells versus other major classes of glial and endothelial cell types that are potentially secretory in the dentate gyrus of adult mice. Of the cell types quantified, we found that GFAP+SOX2+ stellate astrocytes were the most numerous, followed by CD31+ endothelia, GFAP–SOX2+ intermediate progenitors, Olig2+ oligodendrocytes, Iba1+ microglia, and GFAP+SOX2+ radial glia-like neural stem cells. We did not observe any significant sex differences in density of any cell population. Notably, neural stem/progenitor cells were present at a similar density as several cell types known to have potent functional roles via their secretome. These findings may be useful for refining hypotheses regarding the contributions of these cell types to regulating hippocampal function and their potential therapeutic uses. All experimental protocols were approved by the Ohio State University Institutional Animal Care and Use Committee (protocol# 2016A00000068) on July 14, 2016.
format	article
author	Joshua D Rieskamp Patricia Sarchet Bryon M Smith Elizabeth D Kirby
author_facet	Joshua D Rieskamp Patricia Sarchet Bryon M Smith Elizabeth D Kirby
author_sort	Joshua D Rieskamp
title	Estimation of the density of neural, glial, and endothelial lineage cells in the adult mouse dentate gyrus
title_short	Estimation of the density of neural, glial, and endothelial lineage cells in the adult mouse dentate gyrus
title_full	Estimation of the density of neural, glial, and endothelial lineage cells in the adult mouse dentate gyrus
title_fullStr	Estimation of the density of neural, glial, and endothelial lineage cells in the adult mouse dentate gyrus
title_full_unstemmed	Estimation of the density of neural, glial, and endothelial lineage cells in the adult mouse dentate gyrus
title_sort	estimation of the density of neural, glial, and endothelial lineage cells in the adult mouse dentate gyrus
publisher	Wolters Kluwer Medknow Publications
publishDate	2022
url	https://doaj.org/article/6f418ba0229d466fa6fa04784accc2f2
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Estimation of the density of neural, glial, and endothelial lineage cells in the adult mouse dentate gyrus

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