Leishmania donovani triose phosphate isomerase: a potential vaccine target against visceral leishmaniasis.

Visceral leishmaniasis (VL) is one of the most important parasitic diseases with approximately 350 million people at risk. Due to the non availability of an ideal drug, development of a safe, effective, and affordable vaccine could be a solution for control and prevention of this disease. In this st...

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Autores principales:	Pramod K Kushawaha, Reema Gupta, Chandra Dev Pati Tripathi, Prashant Khare, Anil Kumar Jaiswal, Shyam Sundar, Anuradha Dube
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6f425a77b9b44a21983cfdfd7bb199ef
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spelling	oai:doaj.org-article:6f425a77b9b44a21983cfdfd7bb199ef2021-11-18T08:14:17ZLeishmania donovani triose phosphate isomerase: a potential vaccine target against visceral leishmaniasis.1932-620310.1371/journal.pone.0045766https://doaj.org/article/6f425a77b9b44a21983cfdfd7bb199ef2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23049855/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Visceral leishmaniasis (VL) is one of the most important parasitic diseases with approximately 350 million people at risk. Due to the non availability of an ideal drug, development of a safe, effective, and affordable vaccine could be a solution for control and prevention of this disease. In this study, a potential Th1 stimulatory protein- Triose phosphate isomerase (TPI), a glycolytic enzyme, identified through proteomics from a fraction of Leishmania donovani soluble antigen ranging from 89.9-97.1 kDa, was assessed for its potential as a suitable vaccine candidate. The protein- L. donovani TPI (LdTPI) was cloned, expressed and purified which exhibited the homology of 99% with L. infantum TPI. The rLdTPI was further evaluated for its immunogenicity by lymphoproliferative response (LTT), nitric oxide (NO) production and estimation of cytokines in cured Leishmania patients/hamster. It elicited strong LTT response in cured patients as well as NO production in cured hamsters and stimulated remarkable Th1-type cellular responses including IFN-ã and IL-12 with extremely lower level of IL-10 in Leishmania-infected cured/exposed patients PBMCs in vitro. Vaccination with LdTPI-DNA construct protected naive golden hamsters from virulent L. donovani challenge unambiguously (∼90%). The vaccinated hamsters demonstrated a surge in IFN-ã, TNF-á and IL-12 levels but extreme down-regulation of IL-10 and IL-4 along with profound delayed type hypersensitivity and increased levels of Leishmania-specific IgG2 antibody. Thus, the results are suggestive of the protein having the potential of a strong candidate vaccine.Pramod K KushawahaReema GuptaChandra Dev Pati TripathiPrashant KhareAnil Kumar JaiswalShyam SundarAnuradha DubePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e45766 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pramod K Kushawaha Reema Gupta Chandra Dev Pati Tripathi Prashant Khare Anil Kumar Jaiswal Shyam Sundar Anuradha Dube Leishmania donovani triose phosphate isomerase: a potential vaccine target against visceral leishmaniasis.
description	Visceral leishmaniasis (VL) is one of the most important parasitic diseases with approximately 350 million people at risk. Due to the non availability of an ideal drug, development of a safe, effective, and affordable vaccine could be a solution for control and prevention of this disease. In this study, a potential Th1 stimulatory protein- Triose phosphate isomerase (TPI), a glycolytic enzyme, identified through proteomics from a fraction of Leishmania donovani soluble antigen ranging from 89.9-97.1 kDa, was assessed for its potential as a suitable vaccine candidate. The protein- L. donovani TPI (LdTPI) was cloned, expressed and purified which exhibited the homology of 99% with L. infantum TPI. The rLdTPI was further evaluated for its immunogenicity by lymphoproliferative response (LTT), nitric oxide (NO) production and estimation of cytokines in cured Leishmania patients/hamster. It elicited strong LTT response in cured patients as well as NO production in cured hamsters and stimulated remarkable Th1-type cellular responses including IFN-ã and IL-12 with extremely lower level of IL-10 in Leishmania-infected cured/exposed patients PBMCs in vitro. Vaccination with LdTPI-DNA construct protected naive golden hamsters from virulent L. donovani challenge unambiguously (∼90%). The vaccinated hamsters demonstrated a surge in IFN-ã, TNF-á and IL-12 levels but extreme down-regulation of IL-10 and IL-4 along with profound delayed type hypersensitivity and increased levels of Leishmania-specific IgG2 antibody. Thus, the results are suggestive of the protein having the potential of a strong candidate vaccine.
format	article
author	Pramod K Kushawaha Reema Gupta Chandra Dev Pati Tripathi Prashant Khare Anil Kumar Jaiswal Shyam Sundar Anuradha Dube
author_facet	Pramod K Kushawaha Reema Gupta Chandra Dev Pati Tripathi Prashant Khare Anil Kumar Jaiswal Shyam Sundar Anuradha Dube
author_sort	Pramod K Kushawaha
title	Leishmania donovani triose phosphate isomerase: a potential vaccine target against visceral leishmaniasis.
title_short	Leishmania donovani triose phosphate isomerase: a potential vaccine target against visceral leishmaniasis.
title_full	Leishmania donovani triose phosphate isomerase: a potential vaccine target against visceral leishmaniasis.
title_fullStr	Leishmania donovani triose phosphate isomerase: a potential vaccine target against visceral leishmaniasis.
title_full_unstemmed	Leishmania donovani triose phosphate isomerase: a potential vaccine target against visceral leishmaniasis.
title_sort	leishmania donovani triose phosphate isomerase: a potential vaccine target against visceral leishmaniasis.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6f425a77b9b44a21983cfdfd7bb199ef
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Leishmania donovani triose phosphate isomerase: a potential vaccine target against visceral leishmaniasis.

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