Adherent cell remodeling on micropatterns is modulated by Piezo1 channels

Abstract Adherent cells utilize local environmental cues to make decisions on their growth and movement. We have previously shown that HEK293 cells grown on the fibronectin stripe patterns were elongated. Here we show that Piezo1 function is involved in cell spreading. Piezo1 expressing HEK cells pl...

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Autores principales:	Deekshitha Jetta, Mohammad Reza Bahrani Fard, Frederick Sachs, Katie Munechika, Susan Z. Hua
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6f4a2c6f55c84bee8e66b8d6b47449e3
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spelling	oai:doaj.org-article:6f4a2c6f55c84bee8e66b8d6b47449e32021-12-02T13:34:57ZAdherent cell remodeling on micropatterns is modulated by Piezo1 channels10.1038/s41598-021-84427-y2045-2322https://doaj.org/article/6f4a2c6f55c84bee8e66b8d6b47449e32021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84427-yhttps://doaj.org/toc/2045-2322Abstract Adherent cells utilize local environmental cues to make decisions on their growth and movement. We have previously shown that HEK293 cells grown on the fibronectin stripe patterns were elongated. Here we show that Piezo1 function is involved in cell spreading. Piezo1 expressing HEK cells plated on fibronectin stripes elongated, while a knockout of Piezo1 eliminated elongation. Inhibiting Piezo1 conductance using GsMTx4 or Gd3+ blocked cell spreading, but the cells grew thin tail-like extensions along the patterns. Images of GFP-tagged Piezo1 showed plaques of Piezo1 moving to the extrusion edges, co-localized with focal adhesions. Surprisingly, in non-spreading cells Piezo1 was located primarily on the nuclear envelope. Inhibiting the Rho-ROCK pathway also reversibly inhibited cell extension indicating that myosin contractility is involved. The growth of thin extrusion tails did not occur in Piezo1 knockout cells suggesting that Piezo1 may have functions besides acting as a cation channel.Deekshitha JettaMohammad Reza Bahrani FardFrederick SachsKatie MunechikaSusan Z. HuaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Deekshitha Jetta Mohammad Reza Bahrani Fard Frederick Sachs Katie Munechika Susan Z. Hua Adherent cell remodeling on micropatterns is modulated by Piezo1 channels
description	Abstract Adherent cells utilize local environmental cues to make decisions on their growth and movement. We have previously shown that HEK293 cells grown on the fibronectin stripe patterns were elongated. Here we show that Piezo1 function is involved in cell spreading. Piezo1 expressing HEK cells plated on fibronectin stripes elongated, while a knockout of Piezo1 eliminated elongation. Inhibiting Piezo1 conductance using GsMTx4 or Gd3+ blocked cell spreading, but the cells grew thin tail-like extensions along the patterns. Images of GFP-tagged Piezo1 showed plaques of Piezo1 moving to the extrusion edges, co-localized with focal adhesions. Surprisingly, in non-spreading cells Piezo1 was located primarily on the nuclear envelope. Inhibiting the Rho-ROCK pathway also reversibly inhibited cell extension indicating that myosin contractility is involved. The growth of thin extrusion tails did not occur in Piezo1 knockout cells suggesting that Piezo1 may have functions besides acting as a cation channel.
format	article
author	Deekshitha Jetta Mohammad Reza Bahrani Fard Frederick Sachs Katie Munechika Susan Z. Hua
author_facet	Deekshitha Jetta Mohammad Reza Bahrani Fard Frederick Sachs Katie Munechika Susan Z. Hua
author_sort	Deekshitha Jetta
title	Adherent cell remodeling on micropatterns is modulated by Piezo1 channels
title_short	Adherent cell remodeling on micropatterns is modulated by Piezo1 channels
title_full	Adherent cell remodeling on micropatterns is modulated by Piezo1 channels
title_fullStr	Adherent cell remodeling on micropatterns is modulated by Piezo1 channels
title_full_unstemmed	Adherent cell remodeling on micropatterns is modulated by Piezo1 channels
title_sort	adherent cell remodeling on micropatterns is modulated by piezo1 channels
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6f4a2c6f55c84bee8e66b8d6b47449e3
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_version_	1718392742650839040

Adherent cell remodeling on micropatterns is modulated by Piezo1 channels

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