Replication independent formation of extrachromosomal circular DNA in mammalian cell-free system.

Extrachromosomal circular DNA (eccDNA) is a pool of circular double stranded DNA molecules found in all eukaryotic cells and composed of repeated chromosomal sequences. It was proposed to be involved in genomic instability, aging and alternative telomere lengthening. Our study presents novel mammali...

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Autores principales:	Zoya Cohen, Sara Lavi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6f4f0ebc7ee44eaf9423cfea177f545c
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spelling	oai:doaj.org-article:6f4f0ebc7ee44eaf9423cfea177f545c2021-11-25T06:21:47ZReplication independent formation of extrachromosomal circular DNA in mammalian cell-free system.1932-620310.1371/journal.pone.0006126https://doaj.org/article/6f4f0ebc7ee44eaf9423cfea177f545c2009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19568438/?tool=EBIhttps://doaj.org/toc/1932-6203Extrachromosomal circular DNA (eccDNA) is a pool of circular double stranded DNA molecules found in all eukaryotic cells and composed of repeated chromosomal sequences. It was proposed to be involved in genomic instability, aging and alternative telomere lengthening. Our study presents novel mammalian cell-free system for eccDNA generation. Using purified protein extract we show that eccDNA formation does not involve de-novo DNA synthesis suggesting that eccDNA is generated through excision of chromosomal sequences. This process is carried out by sequence-independent enzymes as human protein extract can produce mouse-specific eccDNA from high molecular weight mouse DNA, and vice versa. EccDNA production does not depend on ATP, requires residual amounts of Mg(2+) and is enhanced by double strand DNA breaks.Zoya CohenSara LaviPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 7, p e6126 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zoya Cohen Sara Lavi Replication independent formation of extrachromosomal circular DNA in mammalian cell-free system.
description	Extrachromosomal circular DNA (eccDNA) is a pool of circular double stranded DNA molecules found in all eukaryotic cells and composed of repeated chromosomal sequences. It was proposed to be involved in genomic instability, aging and alternative telomere lengthening. Our study presents novel mammalian cell-free system for eccDNA generation. Using purified protein extract we show that eccDNA formation does not involve de-novo DNA synthesis suggesting that eccDNA is generated through excision of chromosomal sequences. This process is carried out by sequence-independent enzymes as human protein extract can produce mouse-specific eccDNA from high molecular weight mouse DNA, and vice versa. EccDNA production does not depend on ATP, requires residual amounts of Mg(2+) and is enhanced by double strand DNA breaks.
format	article
author	Zoya Cohen Sara Lavi
author_facet	Zoya Cohen Sara Lavi
author_sort	Zoya Cohen
title	Replication independent formation of extrachromosomal circular DNA in mammalian cell-free system.
title_short	Replication independent formation of extrachromosomal circular DNA in mammalian cell-free system.
title_full	Replication independent formation of extrachromosomal circular DNA in mammalian cell-free system.
title_fullStr	Replication independent formation of extrachromosomal circular DNA in mammalian cell-free system.
title_full_unstemmed	Replication independent formation of extrachromosomal circular DNA in mammalian cell-free system.
title_sort	replication independent formation of extrachromosomal circular dna in mammalian cell-free system.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/6f4f0ebc7ee44eaf9423cfea177f545c
work_keys_str_mv	AT zoyacohen replicationindependentformationofextrachromosomalcirculardnainmammaliancellfreesystem AT saralavi replicationindependentformationofextrachromosomalcirculardnainmammaliancellfreesystem
_version_	1718413806073282560

Replication independent formation of extrachromosomal circular DNA in mammalian cell-free system.

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