Whole Organ Blood and Lymphatic Vessels Imaging (WOBLI)

Abstract Thin section histology is limited in providing 3D structural information, particularly of the intricate morphology of the vasculature. Availability of high spatial resolution imaging for thick samples, would overcome the restriction dictated by low light penetration. Our study aimed at opti...

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Autores principales:	Roni Oren, Liat Fellus-Alyagor, Yoseph Addadi, Filip Bochner, Hila Gutman, Shani Blumenreich, Hagit Dafni, Nava Dekel, Michal Neeman, Shlomi Lazar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6f54c7ca5d874fb798a1a7df2d40a6fe
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spelling	oai:doaj.org-article:6f54c7ca5d874fb798a1a7df2d40a6fe2021-12-02T15:08:53ZWhole Organ Blood and Lymphatic Vessels Imaging (WOBLI)10.1038/s41598-018-19663-w2045-2322https://doaj.org/article/6f54c7ca5d874fb798a1a7df2d40a6fe2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19663-whttps://doaj.org/toc/2045-2322Abstract Thin section histology is limited in providing 3D structural information, particularly of the intricate morphology of the vasculature. Availability of high spatial resolution imaging for thick samples, would overcome the restriction dictated by low light penetration. Our study aimed at optimizing the procedure for efficient and affordable tissue clearing, along with an appropriate immunofluorescence labeling that will be applicable for high resolution imaging of blood and lymphatic vessels. The new procedure, termed whole organ blood and lymphatic vessels imaging (WOBLI), is based on two previously reported methods, CLARITY and ScaleA2. We used this procedure for the analysis of isolated whole ovary, uterus, lung and liver. These organs were subjected to passive clearing, following fixation, immunolabeling and embedding in hydrogel. Cleared specimens were immersed in ScaleA2 solution until transparency was achieved and imaged using light sheet microscopy. We demonstrate that WOBLI allows detailed analysis and generation of structural information of the lymphatic and blood vasculature from thick slices and more importantly, from whole organs. We conclude that WOBLI offers the advantages of morphology and fluorescence preservation with efficient clearing. Furthermore, WOBLI provides a robust, cost-effective method for generation of transparent specimens, allowing high resolution, 3D-imaging of blood and lymphatic vessels networks.Roni OrenLiat Fellus-AlyagorYoseph AddadiFilip BochnerHila GutmanShani BlumenreichHagit DafniNava DekelMichal NeemanShlomi LazarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Roni Oren Liat Fellus-Alyagor Yoseph Addadi Filip Bochner Hila Gutman Shani Blumenreich Hagit Dafni Nava Dekel Michal Neeman Shlomi Lazar Whole Organ Blood and Lymphatic Vessels Imaging (WOBLI)
description	Abstract Thin section histology is limited in providing 3D structural information, particularly of the intricate morphology of the vasculature. Availability of high spatial resolution imaging for thick samples, would overcome the restriction dictated by low light penetration. Our study aimed at optimizing the procedure for efficient and affordable tissue clearing, along with an appropriate immunofluorescence labeling that will be applicable for high resolution imaging of blood and lymphatic vessels. The new procedure, termed whole organ blood and lymphatic vessels imaging (WOBLI), is based on two previously reported methods, CLARITY and ScaleA2. We used this procedure for the analysis of isolated whole ovary, uterus, lung and liver. These organs were subjected to passive clearing, following fixation, immunolabeling and embedding in hydrogel. Cleared specimens were immersed in ScaleA2 solution until transparency was achieved and imaged using light sheet microscopy. We demonstrate that WOBLI allows detailed analysis and generation of structural information of the lymphatic and blood vasculature from thick slices and more importantly, from whole organs. We conclude that WOBLI offers the advantages of morphology and fluorescence preservation with efficient clearing. Furthermore, WOBLI provides a robust, cost-effective method for generation of transparent specimens, allowing high resolution, 3D-imaging of blood and lymphatic vessels networks.
format	article
author	Roni Oren Liat Fellus-Alyagor Yoseph Addadi Filip Bochner Hila Gutman Shani Blumenreich Hagit Dafni Nava Dekel Michal Neeman Shlomi Lazar
author_facet	Roni Oren Liat Fellus-Alyagor Yoseph Addadi Filip Bochner Hila Gutman Shani Blumenreich Hagit Dafni Nava Dekel Michal Neeman Shlomi Lazar
author_sort	Roni Oren
title	Whole Organ Blood and Lymphatic Vessels Imaging (WOBLI)
title_short	Whole Organ Blood and Lymphatic Vessels Imaging (WOBLI)
title_full	Whole Organ Blood and Lymphatic Vessels Imaging (WOBLI)
title_fullStr	Whole Organ Blood and Lymphatic Vessels Imaging (WOBLI)
title_full_unstemmed	Whole Organ Blood and Lymphatic Vessels Imaging (WOBLI)
title_sort	whole organ blood and lymphatic vessels imaging (wobli)
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6f54c7ca5d874fb798a1a7df2d40a6fe
work_keys_str_mv	AT ronioren wholeorganbloodandlymphaticvesselsimagingwobli AT liatfellusalyagor wholeorganbloodandlymphaticvesselsimagingwobli AT yosephaddadi wholeorganbloodandlymphaticvesselsimagingwobli AT filipbochner wholeorganbloodandlymphaticvesselsimagingwobli AT hilagutman wholeorganbloodandlymphaticvesselsimagingwobli AT shaniblumenreich wholeorganbloodandlymphaticvesselsimagingwobli AT hagitdafni wholeorganbloodandlymphaticvesselsimagingwobli AT navadekel wholeorganbloodandlymphaticvesselsimagingwobli AT michalneeman wholeorganbloodandlymphaticvesselsimagingwobli AT shlomilazar wholeorganbloodandlymphaticvesselsimagingwobli
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Whole Organ Blood and Lymphatic Vessels Imaging (WOBLI)

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