Genotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine

Abstract Swine are a critical amplifying host involved in human Japanese encephalitis (JE) outbreaks. Cross-genotypic immunogenicity and sterile protection are important for the current genotype III (GIII) virus-derived vaccines in swine, especially now that emerging genotype I (GI) JE virus (JEV) h...

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Autores principales:	Yi-Chin Fan, Jo-Mei Chen, Jen-Wei Lin, Yi-Ying Chen, Guan-Hong Wu, Kuan-Hsuan Su, Ming-Tang Chiou, Shang-Rung Wu, Ji-Hang Yin, Jiunn-Wang Liao, Gwong-Jen J. Chang, Shyan-Song Chiou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6f56990fa5254ab6b18314219e3ec97b
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spelling	oai:doaj.org-article:6f56990fa5254ab6b18314219e3ec97b2021-12-02T16:08:14ZGenotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine10.1038/s41598-018-25596-12045-2322https://doaj.org/article/6f56990fa5254ab6b18314219e3ec97b2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25596-1https://doaj.org/toc/2045-2322Abstract Swine are a critical amplifying host involved in human Japanese encephalitis (JE) outbreaks. Cross-genotypic immunogenicity and sterile protection are important for the current genotype III (GIII) virus-derived vaccines in swine, especially now that emerging genotype I (GI) JE virus (JEV) has replaced GIII virus as the dominant strain. Herein, we aimed to develop a system to generate GI JEV virus-like particles (VLPs) and evaluate the immunogenicity and protection of the GI vaccine candidate in mice and specific pathogen-free swine. A CHO-heparan sulfate-deficient (CHO-HS(-)) cell clone, named 51-10 clone, stably expressing GI-JEV VLP was selected and continually secreted GI VLPs without signs of cell fusion. 51-10 VLPs formed a homogeneously empty-particle morphology and exhibited similar antigenic activity as GI virus. GI VLP-immunized mice showed balanced cross-neutralizing antibody titers against GI to GIV viruses (50% focus-reduction micro-neutralization assay titers 71 to 240) as well as potent protection against GI or GIII virus infection. GI VLP-immunized swine challenged with GI or GIII viruses showed no fever, viremia, or viral RNA in tonsils, lymph nodes, and brains as compared with phosphate buffered saline-immunized swine. We thus conclude GI VLPs can provide sterile protection against GI and GIII viruses in swine.Yi-Chin FanJo-Mei ChenJen-Wei LinYi-Ying ChenGuan-Hong WuKuan-Hsuan SuMing-Tang ChiouShang-Rung WuJi-Hang YinJiunn-Wang LiaoGwong-Jen J. ChangShyan-Song ChiouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi-Chin Fan Jo-Mei Chen Jen-Wei Lin Yi-Ying Chen Guan-Hong Wu Kuan-Hsuan Su Ming-Tang Chiou Shang-Rung Wu Ji-Hang Yin Jiunn-Wang Liao Gwong-Jen J. Chang Shyan-Song Chiou Genotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine
description	Abstract Swine are a critical amplifying host involved in human Japanese encephalitis (JE) outbreaks. Cross-genotypic immunogenicity and sterile protection are important for the current genotype III (GIII) virus-derived vaccines in swine, especially now that emerging genotype I (GI) JE virus (JEV) has replaced GIII virus as the dominant strain. Herein, we aimed to develop a system to generate GI JEV virus-like particles (VLPs) and evaluate the immunogenicity and protection of the GI vaccine candidate in mice and specific pathogen-free swine. A CHO-heparan sulfate-deficient (CHO-HS(-)) cell clone, named 51-10 clone, stably expressing GI-JEV VLP was selected and continually secreted GI VLPs without signs of cell fusion. 51-10 VLPs formed a homogeneously empty-particle morphology and exhibited similar antigenic activity as GI virus. GI VLP-immunized mice showed balanced cross-neutralizing antibody titers against GI to GIV viruses (50% focus-reduction micro-neutralization assay titers 71 to 240) as well as potent protection against GI or GIII virus infection. GI VLP-immunized swine challenged with GI or GIII viruses showed no fever, viremia, or viral RNA in tonsils, lymph nodes, and brains as compared with phosphate buffered saline-immunized swine. We thus conclude GI VLPs can provide sterile protection against GI and GIII viruses in swine.
format	article
author	Yi-Chin Fan Jo-Mei Chen Jen-Wei Lin Yi-Ying Chen Guan-Hong Wu Kuan-Hsuan Su Ming-Tang Chiou Shang-Rung Wu Ji-Hang Yin Jiunn-Wang Liao Gwong-Jen J. Chang Shyan-Song Chiou
author_facet	Yi-Chin Fan Jo-Mei Chen Jen-Wei Lin Yi-Ying Chen Guan-Hong Wu Kuan-Hsuan Su Ming-Tang Chiou Shang-Rung Wu Ji-Hang Yin Jiunn-Wang Liao Gwong-Jen J. Chang Shyan-Song Chiou
author_sort	Yi-Chin Fan
title	Genotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine
title_short	Genotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine
title_full	Genotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine
title_fullStr	Genotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine
title_full_unstemmed	Genotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine
title_sort	genotype i of japanese encephalitis virus virus-like particles elicit sterilizing immunity against genotype i and iii viral challenge in swine
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6f56990fa5254ab6b18314219e3ec97b
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Genotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine

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