Effect of copper nanoparticles and copper ions on the architecture of rainbow trout olfactory mucosa

Olfactory epithelial cells are in direct contact with myriad environmental contaminants which may consequently disrupt their structure and function. Copper ions (Cu2+) and copper nanoparticles (CuNPs) are two types of olfactory neurotoxicants. However, their effects on the structure of olfactory epi...

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Autores principales:	Parastoo Razmara, Gregory G. Pyle
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Copper nanoparticles Olfactory mucosa Mucus Cell junctions Rainbow trout Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/6f58c7c39b7646fabe8488d8eb830f18
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spelling	oai:doaj.org-article:6f58c7c39b7646fabe8488d8eb830f182021-11-06T04:14:16ZEffect of copper nanoparticles and copper ions on the architecture of rainbow trout olfactory mucosa0147-651310.1016/j.ecoenv.2021.112876https://doaj.org/article/6f58c7c39b7646fabe8488d8eb830f182021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S014765132100988Xhttps://doaj.org/toc/0147-6513Olfactory epithelial cells are in direct contact with myriad environmental contaminants which may consequently disrupt their structure and function. Copper ions (Cu2+) and copper nanoparticles (CuNPs) are two types of olfactory neurotoxicants. However, their effects on the structure of olfactory epithelium are largely uninvestigated. The density of olfactory goblet cells in CuNP- and Cu2+ - exposed rainbow trout was assessed using light microscopy throughout time. In both copper (Cu) treatments, the number of goblet cells increased initially over the 24 h exposure and then recovered to normal throughout the 96 h exposure. These data suggested the 96 h exposure to Cu contaminants interfered with protective barrier provided by goblet cells. Nonetheless, lamellar and epithelial thickness of olfactory rosette did not change in the Cu-exposed fish. The gene transcript profile of olfactory mucosa studied by RNA-seq indicated Cu2+ and CuNPs differentially targeted the molecular composition of cell junctions. In the Cu2+ treatment, reduced mRNA abundances of tight junctions, adherens junction, desmosomes and hemidesmosomes, suggest that Cu2+-exposed olfactory mucosal cells had weak junctional complexes. In the CuNP treatment, on the other hand, the transcript abundances of cell junction compositions, except adherens junction, were upregulated. Transcripts associated with gap junctional channels were increased in both Cu treatments. The elevated transcript levels of gap junctions in both Cu treatments suggested that the demand for intercellular communication was increased in the Cu-exposed olfactory mucosa. Overall, our findings suggested that Cu2+ induced greater adverse effects on the molecular composition of olfactory cell junctions relative to CuNPs. Impairment of junctional complexes may disrupt the structural integrity of olfactory mucosa.Parastoo RazmaraGregory G. PyleElsevierarticleCopper nanoparticlesOlfactory mucosaMucusCell junctionsRainbow troutEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 227, Iss , Pp 112876- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Copper nanoparticles Olfactory mucosa Mucus Cell junctions Rainbow trout Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Copper nanoparticles Olfactory mucosa Mucus Cell junctions Rainbow trout Environmental pollution TD172-193.5 Environmental sciences GE1-350 Parastoo Razmara Gregory G. Pyle Effect of copper nanoparticles and copper ions on the architecture of rainbow trout olfactory mucosa
description	Olfactory epithelial cells are in direct contact with myriad environmental contaminants which may consequently disrupt their structure and function. Copper ions (Cu2+) and copper nanoparticles (CuNPs) are two types of olfactory neurotoxicants. However, their effects on the structure of olfactory epithelium are largely uninvestigated. The density of olfactory goblet cells in CuNP- and Cu2+ - exposed rainbow trout was assessed using light microscopy throughout time. In both copper (Cu) treatments, the number of goblet cells increased initially over the 24 h exposure and then recovered to normal throughout the 96 h exposure. These data suggested the 96 h exposure to Cu contaminants interfered with protective barrier provided by goblet cells. Nonetheless, lamellar and epithelial thickness of olfactory rosette did not change in the Cu-exposed fish. The gene transcript profile of olfactory mucosa studied by RNA-seq indicated Cu2+ and CuNPs differentially targeted the molecular composition of cell junctions. In the Cu2+ treatment, reduced mRNA abundances of tight junctions, adherens junction, desmosomes and hemidesmosomes, suggest that Cu2+-exposed olfactory mucosal cells had weak junctional complexes. In the CuNP treatment, on the other hand, the transcript abundances of cell junction compositions, except adherens junction, were upregulated. Transcripts associated with gap junctional channels were increased in both Cu treatments. The elevated transcript levels of gap junctions in both Cu treatments suggested that the demand for intercellular communication was increased in the Cu-exposed olfactory mucosa. Overall, our findings suggested that Cu2+ induced greater adverse effects on the molecular composition of olfactory cell junctions relative to CuNPs. Impairment of junctional complexes may disrupt the structural integrity of olfactory mucosa.
format	article
author	Parastoo Razmara Gregory G. Pyle
author_facet	Parastoo Razmara Gregory G. Pyle
author_sort	Parastoo Razmara
title	Effect of copper nanoparticles and copper ions on the architecture of rainbow trout olfactory mucosa
title_short	Effect of copper nanoparticles and copper ions on the architecture of rainbow trout olfactory mucosa
title_full	Effect of copper nanoparticles and copper ions on the architecture of rainbow trout olfactory mucosa
title_fullStr	Effect of copper nanoparticles and copper ions on the architecture of rainbow trout olfactory mucosa
title_full_unstemmed	Effect of copper nanoparticles and copper ions on the architecture of rainbow trout olfactory mucosa
title_sort	effect of copper nanoparticles and copper ions on the architecture of rainbow trout olfactory mucosa
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/6f58c7c39b7646fabe8488d8eb830f18
work_keys_str_mv	AT parastoorazmara effectofcoppernanoparticlesandcopperionsonthearchitectureofrainbowtroutolfactorymucosa AT gregorygpyle effectofcoppernanoparticlesandcopperionsonthearchitectureofrainbowtroutolfactorymucosa
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Effect of copper nanoparticles and copper ions on the architecture of rainbow trout olfactory mucosa

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