Large Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine

Fluorogenic RNA aptamers such as Chili display strong fluorescence enhancement upon aptamer–ligand complex formation. Here, the authors provide insights into the mechanism of fluorescence activation of Chili by solving the crystal structures of Chili with its bound positively charged ligands DMHBO+...

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Autores principales:	Mateusz Mieczkowski, Christian Steinmetzger, Irene Bessi, Ann-Kathrin Lenz, Alexander Schmiedel, Marco Holzapfel, Christoph Lambert, Vladimir Pena, Claudia Höbartner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6f5b09733cae41098a026686d5776e05
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spelling	oai:doaj.org-article:6f5b09733cae41098a026686d5776e052021-12-02T14:58:18ZLarge Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine10.1038/s41467-021-23932-02041-1723https://doaj.org/article/6f5b09733cae41098a026686d5776e052021-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-23932-0https://doaj.org/toc/2041-1723Fluorogenic RNA aptamers such as Chili display strong fluorescence enhancement upon aptamer–ligand complex formation. Here, the authors provide insights into the mechanism of fluorescence activation of Chili by solving the crystal structures of Chili with its bound positively charged ligands DMHBO+ and DMHBI+, and they reveal that Chili uses an excited state proton transfer mechanism based on time-resolved optical spectroscopy measurements.Mateusz MieczkowskiChristian SteinmetzgerIrene BessiAnn-Kathrin LenzAlexander SchmiedelMarco HolzapfelChristoph LambertVladimir PenaClaudia HöbartnerNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Mateusz Mieczkowski Christian Steinmetzger Irene Bessi Ann-Kathrin Lenz Alexander Schmiedel Marco Holzapfel Christoph Lambert Vladimir Pena Claudia Höbartner Large Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine
description	Fluorogenic RNA aptamers such as Chili display strong fluorescence enhancement upon aptamer–ligand complex formation. Here, the authors provide insights into the mechanism of fluorescence activation of Chili by solving the crystal structures of Chili with its bound positively charged ligands DMHBO+ and DMHBI+, and they reveal that Chili uses an excited state proton transfer mechanism based on time-resolved optical spectroscopy measurements.
format	article
author	Mateusz Mieczkowski Christian Steinmetzger Irene Bessi Ann-Kathrin Lenz Alexander Schmiedel Marco Holzapfel Christoph Lambert Vladimir Pena Claudia Höbartner
author_facet	Mateusz Mieczkowski Christian Steinmetzger Irene Bessi Ann-Kathrin Lenz Alexander Schmiedel Marco Holzapfel Christoph Lambert Vladimir Pena Claudia Höbartner
author_sort	Mateusz Mieczkowski
title	Large Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine
title_short	Large Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine
title_full	Large Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine
title_fullStr	Large Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine
title_full_unstemmed	Large Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine
title_sort	large stokes shift fluorescence activation in an rna aptamer by intermolecular proton transfer to guanine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6f5b09733cae41098a026686d5776e05
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Large Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine

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