Fractionated photothermal therapy in a murine tumor model: comparison with single dose

Marina Simón,1 Kamilla Norregaard,1 Jesper Tranekjær Jørgensen,1 Lene Broeng Oddershede,2 Andreas Kjaer11Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging, Department of Biomedical Sciences, Rigshospitalet and Universi...

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Autores principales:	Simón M, Norregaard K, Jørgensen JT, Oddershede LB, Kjaer A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	hyperthermia cancer nanoparticle photothermal therapy fractionated therapy positron emission tomography Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6f5e87bc350a45e5ae1a0cdc32fb43d0
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spelling	oai:doaj.org-article:6f5e87bc350a45e5ae1a0cdc32fb43d02021-12-02T09:24:57ZFractionated photothermal therapy in a murine tumor model: comparison with single dose1178-2013https://doaj.org/article/6f5e87bc350a45e5ae1a0cdc32fb43d02019-07-01T00:00:00Zhttps://www.dovepress.com/fractionated-photothermal-therapy-in-a-murine-tumor-model-comparison-w-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Marina Simón,1 Kamilla Norregaard,1 Jesper Tranekjær Jørgensen,1 Lene Broeng Oddershede,2 Andreas Kjaer11Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging, Department of Biomedical Sciences, Rigshospitalet and University of Copenhagen, Copenhagen, Denmark; 2Niels Bohr Institute, University of Copenhagen, Copenhagen, DenmarkPurpose: Photothermal therapy (PTT) exploits the light-absorbing properties of nanomaterials such as silica-gold nanoshells (NS) to inflict tumor death through local hyperthermia. However, in in vivo studies of PTT, the heat distribution is often found to be heterogeneous throughout the tumor volume, which leaves parts of the tumor untreated and impairs the overall treatment outcome. As this challenges PTT as a one-dose therapy, this study here investigates if giving the treatment repeatedly, ie, fractionated PTT, increases the efficacy in mice bearing subcutaneous tumors.Methods: The NS heating properties were first optimized in vitro and in vivo. Two fractionated PTT protocols, consisting of two and four laser treatments, respectively, were developed and applied in a murine subcutaneous colorectal tumor model. The efficacy of the two fractionated protocols was evaluated both by longitudinal monitoring of tumor growth and, at an early time point, by positron emission tomography (PET) imaging of 18F-labeled glucose analog 18F-FDG.Results: Overall, there were no significant differences in tumor growth and survival between groups of mice receiving single-dose PTT and fractionated PTT in our study. Nonetheless, some animals did experience inhibited tumor growth or even complete tumor disappearance due to fractionated PTT, and these animals also showed a significant decrease in tumor uptake of 18F-FDG after therapy.Conclusion: This study only found an effect of giving PTT to tumors in fractions compared to a single-dose approach in a few animals. However, many factors can affect the outcome of PTT, and reliable tools for optimization of treatment protocol are needed. Despite the modest treatment effect, our results indicate that 18F-FDG PET/CT imaging can be useful to guide the number of treatment sessions necessary.Keywords: hyperthermia, cancer, nanoparticle, photothermal therapy, fractionated therapy, positron emission tomographySimón MNorregaard KJørgensen JTOddershede LBKjaer ADove Medical Pressarticlehyperthermiacancernanoparticlephotothermal therapyfractionated therapypositron emission tomographyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 5369-5379 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	hyperthermia cancer nanoparticle photothermal therapy fractionated therapy positron emission tomography Medicine (General) R5-920
spellingShingle	hyperthermia cancer nanoparticle photothermal therapy fractionated therapy positron emission tomography Medicine (General) R5-920 Simón M Norregaard K Jørgensen JT Oddershede LB Kjaer A Fractionated photothermal therapy in a murine tumor model: comparison with single dose
description	Marina Simón,1 Kamilla Norregaard,1 Jesper Tranekjær Jørgensen,1 Lene Broeng Oddershede,2 Andreas Kjaer11Department of Clinical Physiology, Nuclear Medicine & PET and Cluster for Molecular Imaging, Department of Biomedical Sciences, Rigshospitalet and University of Copenhagen, Copenhagen, Denmark; 2Niels Bohr Institute, University of Copenhagen, Copenhagen, DenmarkPurpose: Photothermal therapy (PTT) exploits the light-absorbing properties of nanomaterials such as silica-gold nanoshells (NS) to inflict tumor death through local hyperthermia. However, in in vivo studies of PTT, the heat distribution is often found to be heterogeneous throughout the tumor volume, which leaves parts of the tumor untreated and impairs the overall treatment outcome. As this challenges PTT as a one-dose therapy, this study here investigates if giving the treatment repeatedly, ie, fractionated PTT, increases the efficacy in mice bearing subcutaneous tumors.Methods: The NS heating properties were first optimized in vitro and in vivo. Two fractionated PTT protocols, consisting of two and four laser treatments, respectively, were developed and applied in a murine subcutaneous colorectal tumor model. The efficacy of the two fractionated protocols was evaluated both by longitudinal monitoring of tumor growth and, at an early time point, by positron emission tomography (PET) imaging of 18F-labeled glucose analog 18F-FDG.Results: Overall, there were no significant differences in tumor growth and survival between groups of mice receiving single-dose PTT and fractionated PTT in our study. Nonetheless, some animals did experience inhibited tumor growth or even complete tumor disappearance due to fractionated PTT, and these animals also showed a significant decrease in tumor uptake of 18F-FDG after therapy.Conclusion: This study only found an effect of giving PTT to tumors in fractions compared to a single-dose approach in a few animals. However, many factors can affect the outcome of PTT, and reliable tools for optimization of treatment protocol are needed. Despite the modest treatment effect, our results indicate that 18F-FDG PET/CT imaging can be useful to guide the number of treatment sessions necessary.Keywords: hyperthermia, cancer, nanoparticle, photothermal therapy, fractionated therapy, positron emission tomography
format	article
author	Simón M Norregaard K Jørgensen JT Oddershede LB Kjaer A
author_facet	Simón M Norregaard K Jørgensen JT Oddershede LB Kjaer A
author_sort	Simón M
title	Fractionated photothermal therapy in a murine tumor model: comparison with single dose
title_short	Fractionated photothermal therapy in a murine tumor model: comparison with single dose
title_full	Fractionated photothermal therapy in a murine tumor model: comparison with single dose
title_fullStr	Fractionated photothermal therapy in a murine tumor model: comparison with single dose
title_full_unstemmed	Fractionated photothermal therapy in a murine tumor model: comparison with single dose
title_sort	fractionated photothermal therapy in a murine tumor model: comparison with single dose
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/6f5e87bc350a45e5ae1a0cdc32fb43d0
work_keys_str_mv	AT simonm fractionatedphotothermaltherapyinamurinetumormodelcomparisonwithsingledose AT norregaardk fractionatedphotothermaltherapyinamurinetumormodelcomparisonwithsingledose AT jørgensenjt fractionatedphotothermaltherapyinamurinetumormodelcomparisonwithsingledose AT oddershedelb fractionatedphotothermaltherapyinamurinetumormodelcomparisonwithsingledose AT kjaera fractionatedphotothermaltherapyinamurinetumormodelcomparisonwithsingledose
_version_	1718398126944944128

Fractionated photothermal therapy in a murine tumor model: comparison with single dose

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