Potential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool

Nowadays, illegal trafficking and smuggling of animals are among the greatest threats to many avian species. Most commonly smuggled birds belong to parrots, song birds, raptor species and owls. All of these species are protected by national and international legislations. In order to prevent and red...

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Autores principales:	Davitkov Dajana, Vucicevic Milos, Glavinic Uros, Skadric Ivan, Nesic Vladimir, Stevanovic Jevrosima, Stanimirovic Zoran
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2021
Materias:	birds capillary electrophoresis chd1 gene species determination sex determination wildlife trafficking Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/6f67bf2c7a034ec58d0619b0105d2d72
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spelling	oai:doaj.org-article:6f67bf2c7a034ec58d0619b0105d2d722021-11-17T21:27:54ZPotential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool1820-744810.2478/acve-2021-0013https://doaj.org/article/6f67bf2c7a034ec58d0619b0105d2d722021-06-01T00:00:00Zhttps://doi.org/10.2478/acve-2021-0013https://doaj.org/toc/1820-7448Nowadays, illegal trafficking and smuggling of animals are among the greatest threats to many avian species. Most commonly smuggled birds belong to parrots, song birds, raptor species and owls. All of these species are protected by national and international legislations. In order to prevent and reduce wildlife trafficking, DNA methods have become an important forensic tool in species and sex identification. In this study, CHD1R/CHD1F primer pair was used to amplify a part of the CHD1 gene from 65 birds that belong to 43 species. For 36 species this is the first time that the length of CHD1 amplicons was measured for the purpose of species determination. The results were visualized using capillary electrophoresis and enabled simultaneous determination of sex and species. Based on the number of amplicons (two in females, one in males) sex was successfully determined in all species, even in cases where gel electrophoresis failed to give results. Moreover, the species was successfully determined in most bird species based on the species-specific sizes of CHD1 amplicon. The method used in this study is of great importance for veterinary forensic medicine and the prevention of wildlife smuggling. Still, further work is necessary to confirm the effectiveness of the method in all bird species.Davitkov DajanaVucicevic MilosGlavinic UrosSkadric IvanNesic VladimirStevanovic JevrosimaStanimirovic ZoranSciendoarticlebirdscapillary electrophoresischd1 genespecies determinationsex determinationwildlife traffickingVeterinary medicineSF600-1100ENActa Veterinaria, Vol 71, Iss 2, Pp 147-157 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	birds capillary electrophoresis chd1 gene species determination sex determination wildlife trafficking Veterinary medicine SF600-1100
spellingShingle	birds capillary electrophoresis chd1 gene species determination sex determination wildlife trafficking Veterinary medicine SF600-1100 Davitkov Dajana Vucicevic Milos Glavinic Uros Skadric Ivan Nesic Vladimir Stevanovic Jevrosima Stanimirovic Zoran Potential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool
description	Nowadays, illegal trafficking and smuggling of animals are among the greatest threats to many avian species. Most commonly smuggled birds belong to parrots, song birds, raptor species and owls. All of these species are protected by national and international legislations. In order to prevent and reduce wildlife trafficking, DNA methods have become an important forensic tool in species and sex identification. In this study, CHD1R/CHD1F primer pair was used to amplify a part of the CHD1 gene from 65 birds that belong to 43 species. For 36 species this is the first time that the length of CHD1 amplicons was measured for the purpose of species determination. The results were visualized using capillary electrophoresis and enabled simultaneous determination of sex and species. Based on the number of amplicons (two in females, one in males) sex was successfully determined in all species, even in cases where gel electrophoresis failed to give results. Moreover, the species was successfully determined in most bird species based on the species-specific sizes of CHD1 amplicon. The method used in this study is of great importance for veterinary forensic medicine and the prevention of wildlife smuggling. Still, further work is necessary to confirm the effectiveness of the method in all bird species.
format	article
author	Davitkov Dajana Vucicevic Milos Glavinic Uros Skadric Ivan Nesic Vladimir Stevanovic Jevrosima Stanimirovic Zoran
author_facet	Davitkov Dajana Vucicevic Milos Glavinic Uros Skadric Ivan Nesic Vladimir Stevanovic Jevrosima Stanimirovic Zoran
author_sort	Davitkov Dajana
title	Potential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool
title_short	Potential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool
title_full	Potential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool
title_fullStr	Potential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool
title_full_unstemmed	Potential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool
title_sort	potential of inter- and intra-species variability of chd1 gene in birds as a forensic tool
publisher	Sciendo
publishDate	2021
url	https://doaj.org/article/6f67bf2c7a034ec58d0619b0105d2d72
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Potential of Inter- and Intra-Species Variability of CHD1 Gene in Birds as a Forensic Tool

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