Selenium Nanoparticle Protection of Fibroblast Stress: Activation of ATF4 and Bcl-xL Expression

Stanley Chung, Amit K Roy, Thomas J Webster Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USACorrespondence: Thomas J Webster 360 Huntington Avenue, 313 Snell Engineering Center, Boston, MA 02215, USATel +1-617-373-6585Email th.webster@neu.eduBackground: In recent ye...

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Autores principales:	Chung S, Roy AK, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	selenium nanoparticles oxidative stress reductive stress anti-apoptosis stress response Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6f6861c3e0a24d36a3e623723bd97098
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spelling	oai:doaj.org-article:6f6861c3e0a24d36a3e623723bd970982021-12-02T09:04:20ZSelenium Nanoparticle Protection of Fibroblast Stress: Activation of ATF4 and Bcl-xL Expression1178-2013https://doaj.org/article/6f6861c3e0a24d36a3e623723bd970982019-12-01T00:00:00Zhttps://www.dovepress.com/selenium-nanoparticle-protection-of-fibroblast-stress-activation-of-at-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Stanley Chung, Amit K Roy, Thomas J Webster Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USACorrespondence: Thomas J Webster 360 Huntington Avenue, 313 Snell Engineering Center, Boston, MA 02215, USATel +1-617-373-6585Email th.webster@neu.eduBackground: In recent years, selenium nanostructures have been researched due to their antibacterial properties, low toxicity to mammalian cells, and high biological efficacy. However, the clinical implementation of the use of selenium has received mixed results, and there is much work needed to improve the understanding of the biological mechanisms involved in the observed cellular responses.Materials and methods: In this work, an investigation into the mechanistic pathways of selenium nanoparticles (SeNPs) in biological systems was conducted by studying the changes in gene expression of ATF4, Bcl-xL, BAD2, HSP70, and SOD2 in non-cancerous human dermal fibroblasts (HDF) under oxidative stress, nutrient deprivation stress, and no treatment (control) conditions.Results: This study revealed that SeNP incubation led to reduced internal reactive oxygen species (ROS) generation for all conditions tested, thus, providing a protective environment for HDF. At the stress conditions, the expression of ATF4 and Bcl-xL increased for cells treated with SeNP incubation, leading to attenuation of the cells under stress. These results also hint at reductive stress causing a detrimental impact to cell proliferation under routine cell passaging conditions.Conclusion: In summary, this study highlights some possible mechanistic pathways implicated in the action of SeNPs that warrant further investigation (specifically, reducing stress conditions for HDF) and continues to support the promise of SeNPs in a wide range of medical applications.Keywords: selenium nanoparticles, oxidative stress, reductive stress, anti-apoptosis, stress responseChung SRoy AKWebster TJDove Medical Pressarticleselenium nanoparticlesoxidative stressreductive stressanti-apoptosisstress responseMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 9995-10007 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	selenium nanoparticles oxidative stress reductive stress anti-apoptosis stress response Medicine (General) R5-920
spellingShingle	selenium nanoparticles oxidative stress reductive stress anti-apoptosis stress response Medicine (General) R5-920 Chung S Roy AK Webster TJ Selenium Nanoparticle Protection of Fibroblast Stress: Activation of ATF4 and Bcl-xL Expression
description	Stanley Chung, Amit K Roy, Thomas J Webster Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USACorrespondence: Thomas J Webster 360 Huntington Avenue, 313 Snell Engineering Center, Boston, MA 02215, USATel +1-617-373-6585Email th.webster@neu.eduBackground: In recent years, selenium nanostructures have been researched due to their antibacterial properties, low toxicity to mammalian cells, and high biological efficacy. However, the clinical implementation of the use of selenium has received mixed results, and there is much work needed to improve the understanding of the biological mechanisms involved in the observed cellular responses.Materials and methods: In this work, an investigation into the mechanistic pathways of selenium nanoparticles (SeNPs) in biological systems was conducted by studying the changes in gene expression of ATF4, Bcl-xL, BAD2, HSP70, and SOD2 in non-cancerous human dermal fibroblasts (HDF) under oxidative stress, nutrient deprivation stress, and no treatment (control) conditions.Results: This study revealed that SeNP incubation led to reduced internal reactive oxygen species (ROS) generation for all conditions tested, thus, providing a protective environment for HDF. At the stress conditions, the expression of ATF4 and Bcl-xL increased for cells treated with SeNP incubation, leading to attenuation of the cells under stress. These results also hint at reductive stress causing a detrimental impact to cell proliferation under routine cell passaging conditions.Conclusion: In summary, this study highlights some possible mechanistic pathways implicated in the action of SeNPs that warrant further investigation (specifically, reducing stress conditions for HDF) and continues to support the promise of SeNPs in a wide range of medical applications.Keywords: selenium nanoparticles, oxidative stress, reductive stress, anti-apoptosis, stress response
format	article
author	Chung S Roy AK Webster TJ
author_facet	Chung S Roy AK Webster TJ
author_sort	Chung S
title	Selenium Nanoparticle Protection of Fibroblast Stress: Activation of ATF4 and Bcl-xL Expression
title_short	Selenium Nanoparticle Protection of Fibroblast Stress: Activation of ATF4 and Bcl-xL Expression
title_full	Selenium Nanoparticle Protection of Fibroblast Stress: Activation of ATF4 and Bcl-xL Expression
title_fullStr	Selenium Nanoparticle Protection of Fibroblast Stress: Activation of ATF4 and Bcl-xL Expression
title_full_unstemmed	Selenium Nanoparticle Protection of Fibroblast Stress: Activation of ATF4 and Bcl-xL Expression
title_sort	selenium nanoparticle protection of fibroblast stress: activation of atf4 and bcl-xl expression
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/6f6861c3e0a24d36a3e623723bd97098
work_keys_str_mv	AT chungs seleniumnanoparticleprotectionoffibroblaststressactivationofatf4andbclxlexpression AT royak seleniumnanoparticleprotectionoffibroblaststressactivationofatf4andbclxlexpression AT webstertj seleniumnanoparticleprotectionoffibroblaststressactivationofatf4andbclxlexpression
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Selenium Nanoparticle Protection of Fibroblast Stress: Activation of ATF4 and Bcl-xL Expression

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