The Inactivation of Arabidopsis <i>UBC22</i> Results in Abnormal Chromosome Segregation in Female Meiosis, but Not in Male Meiosis

Protein ubiquitination is important for the regulation of meiosis in eukaryotes, including plants. However, little is known about the involvement of E2 ubiquitin-conjugating enzymes in plant meiosis. Arabidopsis UBC22 is a unique E2 enzyme, able to catalyze the formation of ubiquitin dimers through...

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Autores principales:	Ling Cao, Sheng Wang, Lihua Zhao, Yuan Qin, Hong Wang, Yan Cheng
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Arabidopsis chromosome segregation DMC1 female meiosis K11-linked megaspore mother cell Botany QK1-989
Acceso en línea:	https://doaj.org/article/6f69691d022f40268c1f4d7990873825
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spelling	oai:doaj.org-article:6f69691d022f40268c1f4d79908738252021-11-25T18:46:26ZThe Inactivation of Arabidopsis <i>UBC22</i> Results in Abnormal Chromosome Segregation in Female Meiosis, but Not in Male Meiosis10.3390/plants101124182223-7747https://doaj.org/article/6f69691d022f40268c1f4d79908738252021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2418https://doaj.org/toc/2223-7747Protein ubiquitination is important for the regulation of meiosis in eukaryotes, including plants. However, little is known about the involvement of E2 ubiquitin-conjugating enzymes in plant meiosis. Arabidopsis UBC22 is a unique E2 enzyme, able to catalyze the formation of ubiquitin dimers through lysine 11 (K11). Previous work has shown that <i>ubc22</i> mutants are defective in megasporogenesis, with most ovules having no or abnormally functioning megaspores; furthermore, some mutant plants show distinct phenotypes in vegetative growth. In this study, we showed that chromosome segregation and callose deposition were abnormal in mutant female meiosis while male meiosis was not affected. The meiotic recombinase DMC1, required for homologous chromosome recombination, showed a dispersed distribution in mutant female meiocytes compared to the presence of strong foci in WT female meiocytes. Based on an analysis of F1 plants produced from crosses using a mutant as the female parent, about 24% of female mutant gametes had an abnormal content of DNA, resulting in frequent aneuploids among the mutant plants. These results show that UBC22 is critical for normal chromosome segregation in female meiosis but not for male meiosis, and they provide important leads for studying the role of UBC22 and K11-linked ubiquitination.Ling CaoSheng WangLihua ZhaoYuan QinHong WangYan ChengMDPI AGarticleArabidopsischromosome segregationDMC1female meiosisK11-linkedmegaspore mother cellBotanyQK1-989ENPlants, Vol 10, Iss 2418, p 2418 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arabidopsis chromosome segregation DMC1 female meiosis K11-linked megaspore mother cell Botany QK1-989
spellingShingle	Arabidopsis chromosome segregation DMC1 female meiosis K11-linked megaspore mother cell Botany QK1-989 Ling Cao Sheng Wang Lihua Zhao Yuan Qin Hong Wang Yan Cheng The Inactivation of Arabidopsis <i>UBC22</i> Results in Abnormal Chromosome Segregation in Female Meiosis, but Not in Male Meiosis
description	Protein ubiquitination is important for the regulation of meiosis in eukaryotes, including plants. However, little is known about the involvement of E2 ubiquitin-conjugating enzymes in plant meiosis. Arabidopsis UBC22 is a unique E2 enzyme, able to catalyze the formation of ubiquitin dimers through lysine 11 (K11). Previous work has shown that <i>ubc22</i> mutants are defective in megasporogenesis, with most ovules having no or abnormally functioning megaspores; furthermore, some mutant plants show distinct phenotypes in vegetative growth. In this study, we showed that chromosome segregation and callose deposition were abnormal in mutant female meiosis while male meiosis was not affected. The meiotic recombinase DMC1, required for homologous chromosome recombination, showed a dispersed distribution in mutant female meiocytes compared to the presence of strong foci in WT female meiocytes. Based on an analysis of F1 plants produced from crosses using a mutant as the female parent, about 24% of female mutant gametes had an abnormal content of DNA, resulting in frequent aneuploids among the mutant plants. These results show that UBC22 is critical for normal chromosome segregation in female meiosis but not for male meiosis, and they provide important leads for studying the role of UBC22 and K11-linked ubiquitination.
format	article
author	Ling Cao Sheng Wang Lihua Zhao Yuan Qin Hong Wang Yan Cheng
author_facet	Ling Cao Sheng Wang Lihua Zhao Yuan Qin Hong Wang Yan Cheng
author_sort	Ling Cao
title	The Inactivation of Arabidopsis <i>UBC22</i> Results in Abnormal Chromosome Segregation in Female Meiosis, but Not in Male Meiosis
title_short	The Inactivation of Arabidopsis <i>UBC22</i> Results in Abnormal Chromosome Segregation in Female Meiosis, but Not in Male Meiosis
title_full	The Inactivation of Arabidopsis <i>UBC22</i> Results in Abnormal Chromosome Segregation in Female Meiosis, but Not in Male Meiosis
title_fullStr	The Inactivation of Arabidopsis <i>UBC22</i> Results in Abnormal Chromosome Segregation in Female Meiosis, but Not in Male Meiosis
title_full_unstemmed	The Inactivation of Arabidopsis <i>UBC22</i> Results in Abnormal Chromosome Segregation in Female Meiosis, but Not in Male Meiosis
title_sort	inactivation of arabidopsis <i>ubc22</i> results in abnormal chromosome segregation in female meiosis, but not in male meiosis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6f69691d022f40268c1f4d7990873825
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The Inactivation of Arabidopsis <i>UBC22</i> Results in Abnormal Chromosome Segregation in Female Meiosis, but Not in Male Meiosis

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