Multiple pathways suppress non-allelic homologous recombination during meiosis in Saccharomyces cerevisiae.

Recombination during meiosis in the form of crossover events promotes the segregation of homologous chromosomes by providing the only physical linkage between these chromosomes. Recombination occurs not only between allelic sites but also between non-allelic (ectopic) sites. Ectopic recombination is...

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Autores principales:	Miki Shinohara, Akira Shinohara
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6f6cfaa92b2441b2973048f3994ea1eb
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spelling	oai:doaj.org-article:6f6cfaa92b2441b2973048f3994ea1eb2021-11-18T07:47:19ZMultiple pathways suppress non-allelic homologous recombination during meiosis in Saccharomyces cerevisiae.1932-620310.1371/journal.pone.0063144https://doaj.org/article/6f6cfaa92b2441b2973048f3994ea1eb2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23646187/?tool=EBIhttps://doaj.org/toc/1932-6203Recombination during meiosis in the form of crossover events promotes the segregation of homologous chromosomes by providing the only physical linkage between these chromosomes. Recombination occurs not only between allelic sites but also between non-allelic (ectopic) sites. Ectopic recombination is often suppressed to prevent non-productive linkages. In this study, we examined the effects of various mutations in genes involved in meiotic recombination on ectopic recombination during meiosis. RAD24, a DNA damage checkpoint clamp-loader gene, suppressed ectopic recombination in wild type in the same pathway as RAD51. In the absence of RAD24, a meiosis-specific recA homolog, DMC1, suppressed the recombination. Homology search and strand exchange in ectopic recombination occurred when either the RAD51 or the DMC1 recA homolog was absent, but was promoted by RAD52. Unexpectedly, the zip1 mutant, which is defective in chromosome synapsis, showed a decrease, rather than an increase, in ectopic recombination. Our results provide evidence for two types of ectopic recombination: one that occurs in wild-type cells and a second that occurs predominantly when the checkpoint pathway is inactivated.Miki ShinoharaAkira ShinoharaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e63144 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miki Shinohara Akira Shinohara Multiple pathways suppress non-allelic homologous recombination during meiosis in Saccharomyces cerevisiae.
description	Recombination during meiosis in the form of crossover events promotes the segregation of homologous chromosomes by providing the only physical linkage between these chromosomes. Recombination occurs not only between allelic sites but also between non-allelic (ectopic) sites. Ectopic recombination is often suppressed to prevent non-productive linkages. In this study, we examined the effects of various mutations in genes involved in meiotic recombination on ectopic recombination during meiosis. RAD24, a DNA damage checkpoint clamp-loader gene, suppressed ectopic recombination in wild type in the same pathway as RAD51. In the absence of RAD24, a meiosis-specific recA homolog, DMC1, suppressed the recombination. Homology search and strand exchange in ectopic recombination occurred when either the RAD51 or the DMC1 recA homolog was absent, but was promoted by RAD52. Unexpectedly, the zip1 mutant, which is defective in chromosome synapsis, showed a decrease, rather than an increase, in ectopic recombination. Our results provide evidence for two types of ectopic recombination: one that occurs in wild-type cells and a second that occurs predominantly when the checkpoint pathway is inactivated.
format	article
author	Miki Shinohara Akira Shinohara
author_facet	Miki Shinohara Akira Shinohara
author_sort	Miki Shinohara
title	Multiple pathways suppress non-allelic homologous recombination during meiosis in Saccharomyces cerevisiae.
title_short	Multiple pathways suppress non-allelic homologous recombination during meiosis in Saccharomyces cerevisiae.
title_full	Multiple pathways suppress non-allelic homologous recombination during meiosis in Saccharomyces cerevisiae.
title_fullStr	Multiple pathways suppress non-allelic homologous recombination during meiosis in Saccharomyces cerevisiae.
title_full_unstemmed	Multiple pathways suppress non-allelic homologous recombination during meiosis in Saccharomyces cerevisiae.
title_sort	multiple pathways suppress non-allelic homologous recombination during meiosis in saccharomyces cerevisiae.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6f6cfaa92b2441b2973048f3994ea1eb
work_keys_str_mv	AT mikishinohara multiplepathwayssuppressnonallelichomologousrecombinationduringmeiosisinsaccharomycescerevisiae AT akirashinohara multiplepathwayssuppressnonallelichomologousrecombinationduringmeiosisinsaccharomycescerevisiae
_version_	1718422979165028352

Multiple pathways suppress non-allelic homologous recombination during meiosis in Saccharomyces cerevisiae.

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